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XB-ART-32510
Proc Natl Acad Sci U S A 1976 May 01;735:1641-5.
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The development of the action potential mechanism of amphibian neurons isolated in culture.

Spitzer NC, Lamborghini JE.


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Nerve and muscle cells differentiated morphologically, in cultures of dissociated cells prepared from amphibian neural plate and underlying mesoderm (Xenopus laevis, Nieuwkoop and Faber stage 15). Cultures were grown in a defined medium containing sterile Steinberg's salt solution and 0.1% bovine serum albumin, and maintained for periods up to 5 days.

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Species referenced: Xenopus laevis

References [+] :
Hagiwara, Differences in Na and Ca spikes as examined by application of tetrodotoxin, procaine, and manganese ions. 1966, Pubmed