Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The crystalline yolk-platelet proteins and their soluble plasma precursor in an amphibian, Xenopus laevis.
Redshaw MR
,
Follett BK
.
???displayArticle.abstract???
A single lipophosphoprotein complex, vitellogenin, was isolated and purified from the plasma of oestrogen-stimulated female toads by preparative ultracentrifugation and chromatography on TEAE-cellulose (triethylaminoethylcellulose). The protein contains 12% lipid, 1.5% phosphorus, 1.6% calcium and smaller amounts of carbohydrates and biliverdin. In amino acid composition it is identical with total yolk-platelet protein. The platelet protein, however, is fractionated on TEAE-cellulose into two components, a high-molecular-weight lipovitellin and a smaller phosvitin. Analyses of the soluble plasma vitellogenin suggest that it is a complex of two phosvitin molecules covalently bound to one lipovitellin dimer, and that it is the immediate precursor of the yolk proteins, into which it is converted by a molecular rearrangement. Uptake of vitellogenin from the plasma into the growing oocyte, and its subsequent crystallization as a yolk platelet, appear to be enhanced by gonadotrophic hormones.
Andrews,
The gel-filtration behaviour of proteins related to their molecular weights over a wide range.
1965, Pubmed
Andrews,
The gel-filtration behaviour of proteins related to their molecular weights over a wide range.
1965,
Pubmed
Baltus,
Isolation of deoxyribonucleic acid from the yolk platelets of Xenopus laevis oöcyte.
1968,
Pubmed
,
Xenbase
Cook,
Dissociation and subunit size of beta-lipovitellin.
1965,
Pubmed
DAVIS,
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
1964,
Pubmed
Follett,
The effects of oestrogen and gonadotrophins on lipid and protein metabolism in Xenopus laevis Daudin.
1968,
Pubmed
,
Xenbase
HEALD,
Isolation of phosvitin from the plasma of the laying hen.
1963,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
McINDOE,
A lipophosphoprotein complex in hen plasma associated with yolk production.
1959,
Pubmed
Munday,
Oestrogen-induced calcium-binding protein in Xenopus laevis.
1968,
Pubmed
,
Xenbase
ORNSTEIN,
DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.
1964,
Pubmed
Pan,
Vitellogenic blood protein synthesis by insect fat body.
1969,
Pubmed
PIEZ,
A modified procedure for the automatic analysis of amino acids.
1960,
Pubmed
SCHJEIDE,
Proteins induced in plasma by oestrogens.
1960,
Pubmed
SCHJEIDE,
A study of the yolk proteins of frog eggs by physical and chemical means.
1955,
Pubmed
TORIBARA,
The ultrafiltrable calcium of human serum. I. Ultrafiltration methods and normal values.
1957,
Pubmed
URIST,
The partition of calcium and protein in the blood of oviparous vertebrates during estrus.
1961,
Pubmed
Wallace,
Protein incorporation by isolated amphibian oocytes. I. Preliminary studies.
1970,
Pubmed
Wallace,
Studies on amphibian yolk. IX. Xenopus vitellogenin.
1970,
Pubmed
,
Xenbase
Wallace,
Resolution and isolation of avian and amphibian yolk-granule proteins using TEAE-cellulose.
1965,
Pubmed
Wallace,
Studies on amphibian yolk. 8. The estrogen-induced hepatic synthesis of a serum lipophosphoprotein and its selective uptake by the ovary and trasformation into yolk platelet proteins in Xenopus laevis.
1969,
Pubmed
,
Xenbase
WALLACE,
STUDIES ON AMPHIBIAN YOLK. IV. AN ANALYSIS OF THE MAIN-BODY COMPONENT OF YOLK PLATELETS.
1963,
Pubmed
WILLIAMS,
The grouping of serine phosphate residues in phosvitin and casein.
1959,
Pubmed
WINZLER,
Determination of serum glycoproteins.
1955,
Pubmed
