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Nucleic Acids Res
2004 May 25;329:2957-65. doi: 10.1093/nar/gkh620.
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Coupled amplification and degradation of exogenous RNA injected in amphibian oocytes.
Montreau N
,
Vaur S
,
Dautry F
,
Andéol Y
.
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The early development of amphibians takes place in the absence of significant transcription and is controlled at the post-transcriptional level. We have reported that in vitro synthesized transcripts injected into axolotl fertilized eggs or oocytes were not continuously degraded as their abundance apparently fluctuated over time, with detected amounts sometimes higher than initial injected amounts. To further characterize this phenomenon, we have co-injected RNA chain terminators to prevent RNA synthesis. This led to the suppression of fluctuations and to a regular decrease in the amount of transcripts that appeared to be more stable in the presence of inhibitors. These observations indicate a coupling between RNA synthesis and an accelerated degradation. Throughout the time course, cRNA molecules could be detected, and their abundance increased in the early phase of the kinetics, supporting the implication of an RNA-dependent RNA polymerase in an asymmetric amplification process. Finally, when the fate of the injected transcripts was investigated in individual oocytes, we observed an absolute increase in abundance in some but not all oocytes, supporting the existence of a limiting step in the initiation of the RNA amplification stochastic process.
Andéol,
Post-transcriptional control of c-myc RNA during early development analyzed in vivo with a Xenopus-axolotl heterologous system.
1998, Pubmed,
Xenbase
Andéol,
Post-transcriptional control of c-myc RNA during early development analyzed in vivo with a Xenopus-axolotl heterologous system.
1998,
Pubmed
,
Xenbase
Andéol,
Early transcription in different animal species: implication for transition from maternal to zygotic control in development.
1994,
Pubmed
Andéol,
Evidence for a nuclear factor involved in c-myc RNA degradation during axolotl oocyte maturation.
1995,
Pubmed
,
Xenbase
Andéol,
Differential stability of Xenopus c-myc RNA during oogenesis in axolotl Involvement of the 3' untranslated region in vivo.
1995,
Pubmed
,
Xenbase
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980,
Pubmed
Barton,
Complete replication of poliovirus in vitro: preinitiation RNA replication complexes require soluble cellular factors for the synthesis of VPg-linked RNA.
1995,
Pubmed
Bashirullah,
Spatial and temporal control of RNA stability.
2001,
Pubmed
Bosher,
RNA interference: genetic wand and genetic watchdog.
2000,
Pubmed
Busse,
Molecular analysis of the Wnt-1 proto-oncogene in Ambystoma mexicanum (axolotl) embryos.
1993,
Pubmed
Church,
Genomic sequencing.
1984,
Pubmed
Cogoni,
Posttranscriptional gene silencing in Neurospora by a RecQ DNA helicase.
1999,
Pubmed
Day,
Post-transcriptional gene regulatory mechanisms in eukaryotes: an overview.
1998,
Pubmed
Fire,
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.
1998,
Pubmed
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
King,
Expression of the c-myc proto-oncogene during development of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Lefresne,
Evidence for introduction of a variable G1 phase at the midblastula transition during early development in axolotl.
1998,
Pubmed
Makeyev,
Cellular RNA-dependent RNA polymerase involved in posttranscriptional gene silencing has two distinct activity modes.
2002,
Pubmed
Montreau,
Injection of exogenous RNA in amphibian oocytes leads to RNA level fluctuations which are sensitive to cordycepin, an RNA chain elongation terminator.
2003,
Pubmed
,
Xenbase
Neel,
In vivo cooperation between introns during pre-mRNA processing.
1993,
Pubmed
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Schiebel,
RNA-directed RNA polymerase from tomato leaves. II. Catalytic in vitro properties.
1993,
Pubmed
Schiebel,
RNA-directed RNA polymerase from tomato leaves. I. Purification and physical properties.
1993,
Pubmed
Signoret,
Evidence of the first genetic activity required in axolotl development.
1980,
Pubmed
Sijen,
On the role of RNA amplification in dsRNA-triggered gene silencing.
2001,
Pubmed
Smardon,
EGO-1 is related to RNA-directed RNA polymerase and functions in germ-line development and RNA interference in C. elegans.
2000,
Pubmed
Stein,
RNAi: mammalian oocytes do it without RNA-dependent RNA polymerase.
2003,
Pubmed
Tijsterman,
The genetics of RNA silencing.
2002,
Pubmed
Tuschl,
Targeted mRNA degradation by double-stranded RNA in vitro.
1999,
Pubmed
Vaucheret,
Post-transcriptional gene silencing in plants.
2001,
Pubmed
Volloch,
Synthesis of globin RNA in enucleated differentiating murine erythroleukemia cells.
1987,
Pubmed
Volloch,
Cytoplasmic synthesis of globin RNA in differentiated murine erythroleukemia cells: possible involvement of RNA-dependent RNA polymerase.
1986,
Pubmed
Yaniv,
Defining cis-acting elements and trans-acting factors in RNA localization.
2001,
Pubmed
,
Xenbase
