Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Exp Cell Res
2007 May 01;3138:1639-51. doi: 10.1016/j.yexcr.2007.02.018.
Show Gene links
Show Anatomy links
Organization of cytokeratin cytoskeleton and germ plasm in the vegetal cortex of Xenopus laevis oocytes depends on coding and non-coding RNAs: three-dimensional and ultrastructural analysis.
???displayArticle.abstract???
Recent studies discovered a novel structural role of RNA in maintaining the integrity of the mitotic spindle and cellular cytoskeleton. In Xenopus laevis, non-coding Xlsirts and coding VegT RNAs play a structural role in anchoring localized RNAs, maintaining the organization of the cytokeratin cytoskeleton and germinal granules in the oocytevegetal cortex and in subsequent development of the germline in the embryo. We studied the ultrastructural effects of antisense oligonucleotide driven ablation of Xlsirts and VegT RNAs on the organization of the cytokeratin, germ plasm and other components of the vegetal cortex. We developed a novel method to immunolabel and visualize cytokeratin at the electron microscopy level, which allowed us to reconstruct the ultrastructural organization of the cytokeratin network relative to the components of the vegetal cortex in Xenopus oocytes. The removal of Xlsirts and VegT RNAs not only disrupts the cytokeratin cytoskeleton but also has a profound transcript-specific effect on the anchoring and distribution of germ plasm islands and their germinal granules and the arrangement of yolk platelets within the vegetal cortex. We suggest that the cytokeratin cytoskeleton plays a role in anchoring of germ plasm islands within the vegetal cortex and germinal granules within the germ plasm islands.
???displayArticle.pubmedLink???
17376434
???displayArticle.pmcLink???PMC2613015 ???displayArticle.link???Exp Cell Res ???displayArticle.grants???[+]
Alarcón,
RNA anchoring in the vegetal cortex of the Xenopus oocyte.
2001, Pubmed,
Xenbase
Alarcón,
RNA anchoring in the vegetal cortex of the Xenopus oocyte.
2001,
Pubmed
,
Xenbase
Bashirullah,
RNA localization in development.
1998,
Pubmed
,
Xenbase
Blower,
A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.
2005,
Pubmed
,
Xenbase
Clarke,
Cytokeratin intermediate filament organisation and dynamics in the vegetal cortex of living Xenopus laevis oocytes and eggs.
2003,
Pubmed
,
Xenbase
Elinson,
Isolated vegetal cortex from Xenopus oocytes selectively retains localized mRNAs.
1993,
Pubmed
,
Xenbase
Elinson,
Cytoskeleton in Xenopus oocytes and eggs.
1990,
Pubmed
,
Xenbase
Forristall,
Patterns of localization and cytoskeletal association of two vegetally localized RNAs, Vg1 and Xcat-2.
1995,
Pubmed
,
Xenbase
Gard,
The organization and animal-vegetal asymmetry of cytokeratin filaments in stage VI Xenopus oocytes is dependent upon F-actin and microtubules.
1997,
Pubmed
,
Xenbase
Gard,
Confocal microscopy and 3-D reconstruction of the cytoskeleton of Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Gard,
Intermediate filament organization during oogenesis and early development in the clawed frog, Xenopus laevis.
1998,
Pubmed
,
Xenbase
Heasman,
Vegetal localization of maternal mRNAs is disrupted by VegT depletion.
2001,
Pubmed
,
Xenbase
Hudson,
Xpat, a gene expressed specifically in germ plasm and primordial germ cells of Xenopus laevis.
1998,
Pubmed
,
Xenbase
Jansen,
mRNA localization: message on the move.
2001,
Pubmed
King,
Polarizing genetic information in the egg: RNA localization in the frog oocyte.
1999,
Pubmed
,
Xenbase
Kloc,
Formation, architecture and polarity of female germline cyst in Xenopus.
2004,
Pubmed
,
Xenbase
Kloc,
Contribution of METRO pathway localized molecules to the organization of the germ cell lineage.
1998,
Pubmed
,
Xenbase
Kloc,
Potential structural role of non-coding and coding RNAs in the organization of the cytoskeleton at the vegetal cortex of Xenopus oocytes.
2005,
Pubmed
,
Xenbase
Kloc,
Mechanisms of subcellular mRNA localization.
2002,
Pubmed
Kloc,
Mitochondrial ribosomal RNA in the germinal granules in Xenopus embryos revisited.
2001,
Pubmed
,
Xenbase
Kloc,
RNA localization and germ cell determination in Xenopus.
2001,
Pubmed
,
Xenbase
Kloc,
Delocalization of Vg1 mRNA from the vegetal cortex in Xenopus oocytes after destruction of Xlsirt RNA.
1994,
Pubmed
,
Xenbase
Klymkowsky,
Cytokeratin phosphorylation, cytokeratin filament severing and the solubilization of the maternal mRNA Vg1.
1991,
Pubmed
,
Xenbase
Klymkowsky,
MPF-induced breakdown of cytokeratin filament organization in the maturing Xenopus oocyte depends upon the translation of maternal mRNAs.
1989,
Pubmed
,
Xenbase
Larabell,
A new technique for isolation and visualization of the Xenopus egg cortex reveals a complex cytoskeleton.
1993,
Pubmed
,
Xenbase
Larabell,
Freeze-fracture analysis of structural reorganization during meiotic maturation in oocytes of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Palacios,
Getting the message across: the intracellular localization of mRNAs in higher eukaryotes.
2001,
Pubmed
Pondel,
Localized maternal mRNA related to transforming growth factor beta mRNA is concentrated in a cytokeratin-enriched fraction from Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Quaas,
Surface contraction waves (SCWs) in the Xenopus egg are required for the localization of the germ plasm and are dependent upon maternal stores of the kinesin-like protein Xklp1.
2002,
Pubmed
,
Xenbase
Robb,
A kinesin-like protein is required for germ plasm aggregation in Xenopus.
1996,
Pubmed
,
Xenbase
Sardet,
Structure and function of the egg cortex from oogenesis through fertilization.
2002,
Pubmed
,
Xenbase
Savage,
Dynamics of germ plasm localization and its inhibition by ultraviolet irradiation in early cleavage Xenopus embryos.
1993,
Pubmed
,
Xenbase
Stebbings,
Cytoskeleton-dependent transport and localization of mRNA.
2001,
Pubmed
Wessely,
The Xenopus homologue of Bicaudal-C is a localized maternal mRNA that can induce endoderm formation.
2000,
Pubmed
,
Xenbase
Xanthos,
Maternal VegT is the initiator of a molecular network specifying endoderm in Xenopus laevis.
2001,
Pubmed
,
Xenbase
Zhang,
Xenopus VegT RNA is localized to the vegetal cortex during oogenesis and encodes a novel T-box transcription factor involved in mesodermal patterning.
1996,
Pubmed
,
Xenbase
