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The mRNA endonuclease PMR1 initiates mRNA decay by forming a selective complex with its translating substrate mRNA. Previous work showed that the ability of PMR1 to target to polysomes and activate decay depends on the phosphorylation of a tyrosine residue at position 650. The current study shows that c-Src is responsible for activating this mRNA decay pathway. c-Src was recovered with immunoprecipitated PMR1, and it phosphorylates PMR1 in vitro and in vivo. The interaction with c-Src involves two domains of PMR1: Y650 and a series of proline-rich SH3 peptides in the N terminus. In cells with little c-Src, PMR1 targeting to polysomes is induced by constitutively active c-Src but not by inactive forms of the kinase. Similarly, only active c-Src induces PMR1-mediated mRNA decay. Finally, we show that EGF rapidly induces c-Src phosphorylation of PMR1, providing a direct link between tyrosine kinase-mediated signal transduction and mRNA decay.
Binder,
Evidence that the pathway of transferrin receptor mRNA degradation involves an endonucleolytic cleavage within the 3' UTR and does not involve poly(A) tail shortening.
1994, Pubmed
Binder,
Evidence that the pathway of transferrin receptor mRNA degradation involves an endonucleolytic cleavage within the 3' UTR and does not involve poly(A) tail shortening.
1994,
Pubmed
Bremer,
An endonuclease activity similar to Xenopus PMR1 catalyzes the degradation of normal and nonsense-containing human beta-globin mRNA in erythroid cells.
2003,
Pubmed
,
Xenbase
Briata,
p38-dependent phosphorylation of the mRNA decay-promoting factor KSRP controls the stability of select myogenic transcripts.
2005,
Pubmed
Chen,
Stabilization of interleukin-2 mRNA by the c-Jun NH2-terminal kinase pathway.
1998,
Pubmed
Chen,
AU binding proteins recruit the exosome to degrade ARE-containing mRNAs.
2001,
Pubmed
Chernokalskaya,
A polysomal ribonuclease involved in the destabilization of albumin mRNA is a novel member of the peroxidase gene family.
1998,
Pubmed
,
Xenbase
Chou,
Tethering KSRP, a decay-promoting AU-rich element-binding protein, to mRNAs elicits mRNA decay.
2006,
Pubmed
Coller,
General translational repression by activators of mRNA decapping.
2005,
Pubmed
Cunningham,
Polysomal ribonuclease 1 exists in a latent form on polysomes prior to estrogen activation of mRNA decay.
2001,
Pubmed
,
Xenbase
Fenger-Grøn,
Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping.
2005,
Pubmed
Gallouzi,
A novel phosphorylation-dependent RNase activity of GAP-SH3 binding protein: a potential link between signal transduction and RNA stability.
1998,
Pubmed
Hollien,
Decay of endoplasmic reticulum-localized mRNAs during the unfolded protein response.
2006,
Pubmed
Irby,
Role of Src expression and activation in human cancer.
2000,
Pubmed
Ishizawar,
c-Src and cooperating partners in human cancer.
2004,
Pubmed
Jakymiw,
Disruption of GW bodies impairs mammalian RNA interference.
2005,
Pubmed
Liu,
The human and mouse complement of SH2 domain proteins-establishing the boundaries of phosphotyrosine signaling.
2006,
Pubmed
Mukherjee,
The mammalian exosome mediates the efficient degradation of mRNAs that contain AU-rich elements.
2002,
Pubmed
Parker,
The enzymes and control of eukaryotic mRNA turnover.
2004,
Pubmed
Pastori,
Coordinate estrogen-regulated instability of serum protein-coding messenger RNAs in Xenopus laevis.
1991,
Pubmed
,
Xenbase
Rutault,
Combinations of ERK and p38 MAPK inhibitors ablate tumor necrosis factor-alpha (TNF-alpha ) mRNA induction. Evidence for selective destabilization of TNF-alpha transcripts.
2001,
Pubmed
Schmidlin,
The ARE-dependent mRNA-destabilizing activity of BRF1 is regulated by protein kinase B.
2004,
Pubmed
Schoenberg,
Characterization of mRNA endonucleases.
1999,
Pubmed
Stoecklin,
MK2-induced tristetraprolin:14-3-3 complexes prevent stress granule association and ARE-mRNA decay.
2004,
Pubmed
Stoecklin,
ARE-mRNA degradation requires the 5'-3' decay pathway.
2006,
Pubmed
Tharun,
Targeting an mRNA for decapping: displacement of translation factors and association of the Lsm1p-7p complex on deadenylated yeast mRNAs.
2001,
Pubmed
Wang,
Identification of an erythroid-enriched endoribonuclease activity involved in specific mRNA cleavage.
2000,
Pubmed
Wong,
Sprouty2 attenuates epidermal growth factor receptor ubiquitylation and endocytosis, and consequently enhances Ras/ERK signalling.
2002,
Pubmed
Yang,
Endonuclease-mediated mRNA decay requires tyrosine phosphorylation of polysomal ribonuclease 1 (PMR1) for the targeting and degradation of polyribosome-bound substrate mRNA.
2004,
Pubmed
,
Xenbase
Yang,
Endonuclease-mediated mRNA decay involves the selective targeting of PMR1 to polyribosome-bound substrate mRNA.
2004,
Pubmed
Yang,
Polysome-bound endonuclease PMR1 is targeted to stress granules via stress-specific binding to TIA-1.
2006,
Pubmed
