Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The transcriptional potential of the hsp70 heat shock gene promoter is established prior to induction by stress. It has been shown previously that the TBP subunit of TFIID is associated with the TATA element and that RNA polymerase II is paused downstream from the transcription start site. In order to identify new interactions involved in establishing this potentiated state, a detailed analysis of the molecular architecture of a single copy of the hsp70 promoter was performed. A suitably marked promoter was stably integrated using P-element-mediated transformation so as to overcome any ambiguity that might be associated with analyzing the five copies of the endogenous gene. Genomic footprinting using DNase I revealed two previously unidentified interactions. First, the GAGA element located at -120 is protected by protein. Secondly, the pattern of DNase I cleavage in the vicinity of the transcription start is found to bear significant similarity to the pattern associated with binding of purified TFIID. Noting that purified GAGA factor and TFIID interact similarly with the hsp70 and H3 promoters, the architecture of the endogenous H3 promoter was analyzed to determine what interactions might be needed to establish a potentiated state containing a paused polymerase. Despite the detection of TFIID and GAGA on the H3 promoter, no paused polymerase is evident. In addition, no proteins appear to interact with the transcription start. These results suggest that the GAGA factor and TFIID are not sufficient to establish a potentiated state containing paused polymerase and that TFIID interactions downstream from the TATA element could be important for pausing.
Casadaban,
Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast.
1983, Pubmed
Casadaban,
Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast.
1983,
Pubmed
Cohen,
Inducible transcription and puffing in Drosophila melanogaster transformed with hsp70-phage lambda hybrid heat shock genes.
1984,
Pubmed
Dudler,
Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies.
1984,
Pubmed
,
Xenbase
Emanuel,
Transcription factor TFIID recognizes DNA sequences downstream of the TATA element in the Hsp70 heat shock gene.
1993,
Pubmed
Farkas,
The Trithorax-like gene encodes the Drosophila GAGA factor.
1994,
Pubmed
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Garrity,
Effects of different DNA polymerases in ligation-mediated PCR: enhanced genomic sequencing and in vivo footprinting.
1992,
Pubmed
Giardina,
Promoter melting and TFIID complexes on Drosophila genes in vivo.
1992,
Pubmed
Gilmour,
Drosophila nuclear proteins bind to regions of alternating C and T residues in gene promoters.
1989,
Pubmed
Glaser,
Optimal heat-induced expression of the Drosophila hsp26 gene requires a promoter sequence containing (CT)n.(GA)n repeats.
1990,
Pubmed
Grunstein,
Nucleosomes: regulators of transcription.
1990,
Pubmed
Karpov,
Chromatin structure of hsp 70 genes, activated by heat shock: selective removal of histones from the coding region and their absence from the 5' region.
1984,
Pubmed
Lee,
DNA sequence requirements for generating paused polymerase at the start of hsp70.
1992,
Pubmed
Lis,
Protein traffic on the heat shock promoter: parking, stalling, and trucking along.
1993,
Pubmed
Lu,
(CT)n (GA)n repeats and heat shock elements have distinct roles in chromatin structure and transcriptional activation of the Drosophila hsp26 gene.
1993,
Pubmed
Lu,
Promoter sequence containing (CT)n.(GA)n repeats is critical for the formation of the DNase I hypersensitive sites in the Drosophila hsp26 gene.
1992,
Pubmed
Mueller,
In vivo footprinting of a muscle specific enhancer by ligation mediated PCR.
1989,
Pubmed
Nacheva,
Change in the pattern of histone binding to DNA upon transcriptional activation.
1989,
Pubmed
O'Brien,
Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation.
1994,
Pubmed
Purnell,
TFIID sequence recognition of the initiator and sequences farther downstream in Drosophila class II genes.
1994,
Pubmed
Rabindran,
Regulation of heat shock factor trimer formation: role of a conserved leucine zipper.
1993,
Pubmed
Rasmussen,
In vivo transcriptional pausing and cap formation on three Drosophila heat shock genes.
1993,
Pubmed
Rougvie,
Postinitiation transcriptional control in Drosophila melanogaster.
1990,
Pubmed
Rubin,
Genetic transformation of Drosophila with transposable element vectors.
1982,
Pubmed
Samal,
Chromatin structure of the histone genes of D. melanogaster.
1981,
Pubmed
Schedl,
Two hybrid plasmids with D. melanogaster DNA sequences complementary to mRNA coding for the major heat shock protein.
1978,
Pubmed
Simon,
Determinants of heat shock-induced chromosome puffing.
1985,
Pubmed
Sypes,
Protein/DNA crosslinking of a TFIID complex reveals novel interactions downstream of the transcription start.
1994,
Pubmed
Thomas,
Protein/DNA architecture of the DNase I hypersensitive region of the Drosophila hsp26 promoter.
1988,
Pubmed
Tsukiyama,
ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor.
1994,
Pubmed
Usheva,
Specific interaction between the nonphosphorylated form of RNA polymerase II and the TATA-binding protein.
1992,
Pubmed
Worcel,
Chromatin fine structure of the histone gene complex of Drosophila melanogaster.
1983,
Pubmed
Wu,
The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
1980,
Pubmed
Wu,
An exonuclease protection assay reveals heat-shock element and TATA box DNA-binding proteins in crude nuclear extracts.
,
Pubmed
