Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The structure and regulation of phosphoglucose isomerase in Saccharomyces cerevisiae.
Green JB
,
Wright AP
,
Cheung WY
,
Lancashire WE
,
Hartley BS
.
???displayArticle.abstract???
We have cloned and sequenced the PGI1 gene, encoding phosphoglucose isomerase (E.C.5.3.1.9), from Saccharomyces cerevisiae. The nucleotide sequence predicts subunits of 554 amino acids with a molecular weight of 61,230. Both the size and amino acid composition correlate well with measurements from purified protein. We have compared the PGI1 protein with the predicted sequence for pig musclePGI. In spite of some evolutionary divergence the proteins are very similar and there are some highly conserved regions, two of which have been implicated in the active site. It has been suggested that PGI exists in two or more isozyme forms in S. cerevisiae and analogy with ADR2/ADC1 suggests that such PGI isozymes might also be differentially regulated during glycolytic/gluconeogenic growth. We have used accurate quantitation of PGI1 mRNA and gene fusions of PGI1 to the lacZ gene of Escherichia coli to show that PGI1 transcription is regulated neither between glycolytic and gluconeogenic growth nor between exponential and stationary phase. The complete lack of PGI activity in PGI1 deletion mutants and of differential regulation suggests that the isozymes of PGI might result merely from processing of the PGI1 gene product.
Achari,
Glucose-6-phosphate isomerase.
1981,
Pubmed
Aguilera,
Deletion of the phosphoglucose isomerase structural gene makes growth and sporulation glucose dependent in Saccharomyces cerevisiae.
1986,
Pubmed
Aguilera,
Isolation and molecular analysis of the phosphoglucose isomerase structural gene of Saccharomyces cerevisiae.
1986,
Pubmed
Beggs,
Transformation of yeast by a replicating hybrid plasmid.
1978,
Pubmed
Bennetzen,
The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase.
1982,
Pubmed
Benoist,
The ovalbumin gene-sequence of putative control regions.
1980,
Pubmed
Birnboim,
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
1979,
Pubmed
Bruch,
Matrix-bound phosphoglucose isomerase. Formation and properties of monomers and hybrids.
1976,
Pubmed
Casadaban,
Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast.
1983,
Pubmed
Chaput,
The neurotrophic factor neuroleukin is 90% homologous with phosphohexose isomerase.
1988,
Pubmed
Chou,
Beta-turns in proteins.
1977,
Pubmed
Cini,
Molecular basis of the isozymes of bovine glucose-6-phosphate isomerase.
1986,
Pubmed
Clewell,
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
1969,
Pubmed
Denis,
mRNA levels for the fermentative alcohol dehydrogenase of Saccharomyces cerevisiae decrease upon growth on a nonfermentable carbon source.
1983,
Pubmed
Dickinson,
The cdc30 mutation in Saccharomyces cerevisiae results in a temperature-sensitive isoenzyme of phosphoglucose isomerase.
1987,
Pubmed
Dobson,
Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae.
1982,
Pubmed
Entian,
Cloning of hexokinase structural genes from Saccharomyces cerevisiae mutants with regulatory mutations responsible for glucose repression.
1985,
Pubmed
Entian,
Glycolytic enzymes and intermediates in carbon catabolite repression mutants of Saccharomyces cerevisiae.
1980,
Pubmed
Faik,
Mouse glucose-6-phosphate isomerase and neuroleukin have identical 3' sequences.
1988,
Pubmed
Feinberg,
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
1984,
Pubmed
Gannon,
Organisation and sequences at the 5' end of a cloned complete ovalbumin gene.
1979,
Pubmed
Guarente,
Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast.
1983,
Pubmed
Jung,
Exons encode functional and structural units of chicken lysozyme.
1980,
Pubmed
Kempe,
Physical and chemical properties of yeast phosphoglucose isomerase isoenzymes.
1974,
Pubmed
Lancashire,
Petite-negative mutants of Saccharomyces cerevisiae.
1981,
Pubmed
Lowe,
The subunit structure of phosphoglucose isomerase from bakers' yeast.
1975,
Pubmed
Maitra,
Genetic studies with a phosphoglucose isomerase mutant of Saccharomyces cerevisiae.
1977,
Pubmed
Maitra,
A kinetic study of glycolytic enzyme synthesis in yeast.
1971,
Pubmed
Messing,
A system for shotgun DNA sequencing.
1981,
Pubmed
Mount,
Improved programs for DNA and protein sequence analysis on the IBM personal computer and other standard computer systems.
1986,
Pubmed
Nasmyth,
Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.
1980,
Pubmed
O'Connell,
Affinity labeling of phosphoglucose isomerase by 1,2-anhydrohexitol-6-phosphates.
1973,
Pubmed
Pon,
Molecular weight and amino acid composition of five-times-crystallized phosphoglucose isomerase from rabbit skeletal muscle.
1970,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schmitt,
The synthesis of yeast pyruvate decarboxylase is regulated by large variations in the messenger RNA level.
1983,
Pubmed
Schnackerz,
Pyridoxal 5'-phosphate as a site-specific protein reagent for a catalytically critical lysine residue in rabbit muscle phosphoglucose isomerase.
1971,
Pubmed
Shaw,
Crystallographic structure analysis of glucose 6-phosphate isomerase at 3-5 A resolution.
1977,
Pubmed
Stinchcomb,
Centromeric DNA from Saccharomyces cerevisiae.
1982,
Pubmed
Tschumper,
Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.
1980,
Pubmed
Zaret,
DNA sequence required for efficient transcription termination in yeast.
1982,
Pubmed
