Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Properties of the metal ion binding sites of Zn-transcription factor IIIA (TFIIIA) were investigated to understand the potential of this type of zinc finger to undergo reactions that remove Zn(2+) from the protein. Zn-TFIIIA was purified from E. coli containing the cloned sequence for Xenopus laevis oocyte TFIIIA and its stoichiometry of bound Zn(2+) was shown to depend on the details of the isolation process. The average dissociation constant of Zn(2+) in Zn-TFIIIIA was 10(-7). The dissociation constant for Zn-F3, the third finger from the N-terminus of TFIIIA, was 1.0 x 10(-8). The reactivity of Zn-TFIIIA with a series of metal binding ligands, including 2-carboxy-2'-hydroxy-5'-sulfoformazylbenzene (zincon), 4-(2-pyridylazo)-resorcinol (PAR), and 3-ethoxy-2-oxo-butyraldehyde-bis-(N(4)-dimethylthiosemicarbazone) (H(2)KTSM(2)) revealed similar kinetics. The reactivity of PAR with Zn-TFIIIA declined substantially when the protein was bound to the internal control region (ICR) of the 5S ribosomal DNA. Both Cd(2+) and Pb(2+) disrupt TFIIIA binding to its cognate DNA sequence. The Pb(2+) dissociation constant of Pb-F3 was measured as 2.5 x 10(-8). According to NMR spectroscopy, F3 does not fold into a regular conformation in the presence of Pb(2+).
Berg,
Proposed structure for the zinc-binding domains from transcription factor IIIA and related proteins.
1988, Pubmed
Berg,
Proposed structure for the zinc-binding domains from transcription factor IIIA and related proteins.
1988,
Pubmed
Berg,
Zinc finger domains: hypotheses and current knowledge.
1990,
Pubmed
Bieker,
Physical properties and DNA-binding stoichiometry of a 5 S gene-specific transcription factor.
1984,
Pubmed
,
Xenbase
Bittel,
The DNA binding activity of metal response element-binding transcription factor-1 is activated in vivo and in vitro by zinc, but not by other transition metals.
1998,
Pubmed
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Brown,
The primary structure of transcription factor TFIIIA has 12 consecutive repeats.
1985,
Pubmed
,
Xenbase
Chen,
Structural and functional heterogeneity among the zinc fingers of human MRE-binding transcription factor-1.
1998,
Pubmed
Clemens,
Relative contributions of the zinc fingers of transcription factor IIIA to the energetics of DNA binding.
1994,
Pubmed
,
Xenbase
Del Río,
High yield purification of active transcription factor IIIA expressed in E. coli.
1991,
Pubmed
,
Xenbase
Diakun,
EXAFS study of the zinc-binding sites in the protein transcription factor IIIA.
,
Pubmed
,
Xenbase
ELLMAN,
A colorimetric method for determining low concentrations of mercaptans.
1958,
Pubmed
Elrod-Erickson,
Zif268 protein-DNA complex refined at 1.6 A: a model system for understanding zinc finger-DNA interactions.
1996,
Pubmed
Fairall,
The crystal structure of a two zinc-finger peptide reveals an extension to the rules for zinc-finger/DNA recognition.
1993,
Pubmed
Foster,
Domain packing and dynamics in the DNA complex of the N-terminal zinc fingers of TFIIIA.
1997,
Pubmed
Han,
Xenopus transcription factor IIIA. Evidence for heterogeneity of Zn2+ binding affinities and specific labeling of cysteine 287.
1990,
Pubmed
,
Xenbase
Hanas,
Lead inhibition of DNA-binding mechanism of Cys(2)His(2) zinc finger proteins.
1999,
Pubmed
,
Xenbase
Hanas,
Inhibition of transcription factor IIIA-DNA interactions by xenobiotic metal ions.
1996,
Pubmed
,
Xenbase
Hanas,
Xenopus transcription factor A requires zinc for binding to the 5 S RNA gene.
1983,
Pubmed
,
Xenbase
Hanas,
Conformation states of Xenopus transcription factor IIIA.
1989,
Pubmed
,
Xenbase
Hartwig,
Interactions by carcinogenic metal compounds with DNA repair processes: toxicological implications.
2002,
Pubmed
Huang,
Interprotein metal exchange between transcription factor IIIa and apo-metallothionein.
2004,
Pubmed
Kochoyan,
Architectural rules of the zinc-finger motif: comparative two-dimensional NMR studies of native and "aromatic-swap" domains define a "weakly polar switch".
1991,
Pubmed
Krepkiy,
Interaction of Cd2+ with Zn finger 3 of transcription factor IIIA: structures and binding to cognate DNA.
2004,
Pubmed
,
Xenbase
Krepkiy,
Model reactions of Cr (VI) with DNA mediated by thiol species.
2001,
Pubmed
,
Xenbase
Kuwahara,
Role of the zinc(II) ions in the structure of the three-finger DNA binding domain of the Sp1 transcription factor.
1990,
Pubmed
Laib,
Formation and characterization of aurothioneins: Au,Zn,Cd-thionein, Au,Cd-thionein, and (thiomalato-Au)chi-thionein.
1985,
Pubmed
Lee,
Three-dimensional solution structure of a single zinc finger DNA-binding domain.
1989,
Pubmed
,
Xenbase
Liao,
Specific interaction of the first three zinc fingers of TFIIIA with the internal control region of the Xenopus 5 S RNA gene.
1992,
Pubmed
,
Xenbase
Makowski,
The interactions of zinc, nickel, and cadmium with Xenopus transcription factor IIIA, assessed by equilibrium dialysis.
1992,
Pubmed
,
Xenbase
Michael,
Metal binding and folding properties of a minimalist Cys2His2 zinc finger peptide.
1992,
Pubmed
Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Muñoz,
Characterization of the cadmium complex of peptide 49-61: a putative nucleation center for cadmium-induced folding in rabbit liver metallothionein IIA.
1999,
Pubmed
Narayan,
Structures of zinc finger domains from transcription factor Sp1. Insights into sequence-specific protein-DNA recognition.
1997,
Pubmed
Nolte,
Differing roles for zinc fingers in DNA recognition: structure of a six-finger transcription factor IIIA complex.
1998,
Pubmed
,
Xenbase
Omichinski,
High-resolution three-dimensional structure of a single zinc finger from a human enhancer binding protein in solution.
1990,
Pubmed
Outten,
Femtomolar sensitivity of metalloregulatory proteins controlling zinc homeostasis.
2001,
Pubmed
Párraga,
Zinc-dependent structure of a single-finger domain of yeast ADR1.
1988,
Pubmed
Pavletich,
Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A.
1991,
Pubmed
Petering,
Cadmium and lead interactions with transcription factor IIIA from Xenopus laevis: a model for zinc finger protein reactions with toxic metal ions and metallothionein.
2000,
Pubmed
,
Xenbase
Posewitz,
Properties of the Sp1 zinc finger 3 peptide: coordination chemistry, redox reactions, and metal binding competition with metallothionein.
1995,
Pubmed
Predki,
Effect of replacement of "zinc finger" zinc on estrogen receptor DNA interactions.
1992,
Pubmed
Razmiafshari,
Utilization of a synthetic peptide as a tool to study the interaction of heavy metals with the zinc finger domain of proteins critical for gene expression in the developing brain.
2000,
Pubmed
Razmiafshari,
NMR identification of heavy metal-binding sites in a synthetic zinc finger peptide: toxicological implications for the interactions of xenobiotic metals with zinc finger proteins.
2001,
Pubmed
Roesijadi,
Modulation of DNA binding of a tramtrack zinc finger peptide by the metallothionein-thionein conjugate pair.
1998,
Pubmed
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Shang,
Zinc release from Xenopus transcription factor IIIA induced by chemical modifications.
1989,
Pubmed
,
Xenbase
Solaiman,
Reactions of 3-ethoxy-2-oxobutyraldehyde bis(N4-dimethylthiosemicarbazonato) cadmium with tumor cells.
1979,
Pubmed
Sunderman,
Finger-loops, oncogenes, and metals. Claude Passmore Brown memorial lecture.
1988,
Pubmed
Wuttke,
Solution structure of the first three zinc fingers of TFIIIA bound to the cognate DNA sequence: determinants of affinity and sequence specificity.
1997,
Pubmed
,
Xenbase
Zwieb,
Absence of substantial bending in Xenopus laevis transcription factor IIIA-DNA complexes.
1990,
Pubmed
,
Xenbase
