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Nuclei of vitellogenic oocytes of the frog, Xenopus laevis, contain a prominent protein, representing about 10% of nuclear protein, which is characterized by a polypeptide of M(r) 30,000. This protein is highly phosphorylated and acidic, displays several isoelectric variants with pI values ranging from 4.7 to 5.3, shows a high thermostability, is not stably complexed with other proteins, and is readily extracted in buffer solutions. Guinea pig antibodies against this protein have allowed its specific immunoprecipitation and localization by immunofluorescence microscopy, using both frozen tissue sections and cells grown in vitro. The protein is located almost exclusively in the nucleus where it appears to be spread throughout the nuclear interior. It is also a major nucleus-specific protein in vitellogenic and previtellogenic oocytes of other amphibian species as well as in other cell types, including hepatocytes, follicle epithelial cells, and cultured Xenopus cells, but is not detected in nuclei of transcriptionally inactive cells such as erythrocytes and spermatids. An immunologically related, if not identical, protein occurs in nuclei of higher vertebrate cells, including mammals. The properties of this abundant nuclear phosphoprotein and its possible relationship to the "nucleosome assembly factor" protein are discussed. It is suggested that this soluble protein serves a general nuclear function.
Bonner,
Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.
1975, Pubmed,
Xenbase
Bonner,
Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.
1975,
Pubmed
,
Xenbase
Bonner,
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
1974,
Pubmed
Bustin,
Antibodies against chromosomal HMG proteins stain the cytoplasm of mammalian cells.
1979,
Pubmed
Clark,
Diffusible and bound actin nuclei of Xenopus laevis oocytes.
1977,
Pubmed
,
Xenbase
Colman,
Synthesis of RNA in oocytes of Xenopus laevis during culture in vitro.
1974,
Pubmed
,
Xenbase
De Robertis,
Intracellular migration of nuclear proteins in Xenopus oocytes.
1978,
Pubmed
,
Xenbase
De Robertis,
Somatic nuclei in amphibian oocytes: evidence for selective gene expression.
1977,
Pubmed
,
Xenbase
Franke,
Different intermediate-sized filaments distinguished by immunofluorescence microscopy.
1978,
Pubmed
Franke,
Intermediate-sized filaments of human endothelial cells.
1979,
Pubmed
Franke,
Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates.
1979,
Pubmed
,
Xenbase
Franke,
Antibody to prekeratin. Decoration of tonofilament like arrays in various cells of epithelial character.
1978,
Pubmed
Goldknopf,
Isopeptide linkage between nonhistone and histone 2A polypeptides of chromosomal conjugate-protein A24.
1977,
Pubmed
Gurdon,
Nuclear transplantation and the control of gene activity in animal development.
1970,
Pubmed
Krohne,
The major polypeptides of the nuclear pore complex.
1978,
Pubmed
,
Xenbase
Krohne,
Localization of a nuclear envelope-associated protein by indirect immunofluorescence microscopy using antibodies against a major polypeptide from rat liver fractions enriched in nuclear envelope-associated material.
1978,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Laskey,
Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA.
1978,
Pubmed
,
Xenbase
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Maundrell,
Proteins of the newt oocyte nucleus: analysis of the nonhistone proteins from lampbrush chromosomes, nucleoli and nuclear sap.
1975,
Pubmed
McKnight,
A colorimetric method for the determination of submicrogram quantities of protein.
1977,
Pubmed
Merriam,
The germinal vesicle nucleus of Xenopus laevis oocytes as a selective storage receptacle for proteins.
1976,
Pubmed
,
Xenbase
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
Paine,
Nuclear envelope permeability.
1975,
Pubmed
Thomas,
An octamer of histones in chromatin and free in solution.
1975,
Pubmed
Watson,
The purification, characterization and partial sequence determination of a trout testis non-histone protein, HMG-T.
1977,
Pubmed
