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???displayArticle.abstract??? Nr2e3 is a nuclear hormone receptor that is involved in rod photoreceptor differentiation. The Nr2e3 gene was previously identified in humans, mice, zebrafish and chicken. In all species, Nr2e3 expression is restricted to the retina and is believed to have a role in rod photoreceptor specification and maintenance. Here we report the identification and characterization of the Xenopus Nr2e3. We found that Nr2e3 is primarily expressed in developing rod photoreceptors. In contrast to other species, Nr2e3 is also expressed in the notochord and pineal gland during Xenopus laevis development.
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17486544
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Fig. 1. Amino acid sequence comparison of Nr2e3 between different species. (A) The sequence of the Xenopus tropicalis Nr2e3 (xtNr2e3) gene product (Accession number: DQ906161) is compared to that of the chicken, mouse and human. Single underline denotes the conserved ligand binding domain (LBD) and the double underline denotes the conserved DNA binding domain (DBD). (B) Phylogenic analysis of xtNr2e3 and the related orphan nuclear receptors Nr2e1 (Tlx) and Nr2f2 (COUP TF II). Phylogenetic analysis was performed from ClustalW alignment using the Phylip tree format in the Tree Top program (http:// www.genebee.msu.su/services/phtree_reduced.html). Species abbreviations: xt, Xenopus tropicalis; c, chicken; m, mouse; h, human; xl, Xenopus laevis.
Fig. 2 a-f. Expression of Nr2e3 in X. laevis embryos during development. (A-H) Lateral views of embryos subjected to wholemount in situ hybridization using antisense riborprobes specific for Nr2e3 (A-C), Tlx (nr2e1)(D-F).
note figure 2 g-i shown as separte image xb-img-77278
Fig. 2 (continued.) Lateral views of embryos subjected to wholemount in situ hybridization using antisense riborprobes specific for Rx (G). Arrow denotes retinal expression and the arrowhead denotes pineal gland expression(H, I). Expression of Nr2e3 in X. laevis embryos during development.
Transverse sections of an embryo at stage 30/31 after wholemount in situ hybridization. Transverse section through the head showing retinal expression (arrow) (I) Transverse section around the gut area showing strong notochord expression (arrow). In (A- G), riboprobes and developmental stages are indi- cated in the lower left and upper right corner of each
panel, respectively.
Fig. 3. Nr2e3 and Tlx (Nr2e1) expression in retinal sections. In situ hybridization using paraffin sections of embryos at various stages during X. laevis development probed for Nr2e3 (A-C) and Tlx (Nr2e1) (D-F) expression. Nr2e3 is expressed in the neural retina, but not in the CMZ (A-C). Retinal expression of Tlx is restricted to the CMZ by stage 45 (arrows in (D-F). Retinal layers and the lens are labeled with a letter abbreviation: L, lens; G, ganglion cell layer; I, inner nuclear layer; P, photoreceptor layer.
Fig. 4. Co-expression of Nr2e3 and Rhodopsin in rods. (A) Double in situ hybridization for Nr2e3 (purple) and Rhodopsin (red) in a section of an eye from a stage 45 embryo. Arrows denote the overlap between Nr2e3 and Rhodopsin expression. (B) Higher magnification (optical and digital) of the picture in (A) showing the region of overlap between Nr2e3 and Rhodopsin expression (arrowhead).
