Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Actin is involved in endocytosis in organisms ranging from yeast to mammals. In activated Xenopus eggs, exocytosing cortical granules (CGs) are surrounded by actin "coats," which compress the exocytosing compartments, resulting in compensatory endocytosis. Here, we examined the roles of two myosins in actin coat compression. Myosin-2 is recruited to exocytosing CGs late in coat compression. Inhibition of myosin-2 slows coat compression without affecting actin assembly. This differs from phenotype induced by inhibition of actin assembly, where exocytosing CGs are trapped at the plasma membrane (PM) completely. Thus, coat compression is likely driven in part by actin assembly itself, but it requires myosin-2 for efficient completion. In contrast to myosin-2, the long-tailed myosin-1e is recruited to exocytosing CGs immediately after egg activation. Perturbation of myosin-1e results in partial actin coat assembly and induces CG collapse into the PM. Intriguingly, simultaneous inhibition of actin assembly and myosin-1e prevents CG collapse. Together, the results show that myosin-1e and myosin-2 are part of an intricate machinery that coordinates coat compression at exocytosing CGs.
Becker,
Reorganization of filamentous actin and myosin-II in zebrafish eggs correlates temporally and spatially with cortical granule exocytosis.
1999, Pubmed
Becker,
Reorganization of filamentous actin and myosin-II in zebrafish eggs correlates temporally and spatially with cortical granule exocytosis.
1999,
Pubmed
Benink,
Concentric zones of active RhoA and Cdc42 around single cell wounds.
2005,
Pubmed
,
Xenbase
Bose,
Glucose transporter recycling in response to insulin is facilitated by myosin Myo1c.
,
Pubmed
Burkel,
Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin.
2007,
Pubmed
,
Xenbase
Doberstein,
Localization and specificity of the phospholipid and actin binding sites on the tail of Acanthamoeba myosin IC.
1992,
Pubmed
Durrbach,
Brush border myosin-I truncated in the motor domain impairs the distribution and the function of endocytic compartments in an hepatoma cell line.
1996,
Pubmed
Engqvist-Goldstein,
Actin assembly and endocytosis: from yeast to mammals.
2003,
Pubmed
Jung,
The actin binding site in the tail domain of Dictyostelium myosin IC (myoC) resides within the glycine- and proline-rich sequence (tail homology region 2).
1994,
Pubmed
Kaksonen,
A modular design for the clathrin- and actin-mediated endocytosis machinery.
2005,
Pubmed
Kelley,
Xenopus nonmuscle myosin heavy chain isoforms have different subcellular localizations and enzymatic activities.
1996,
Pubmed
,
Xenbase
Kimura,
Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase).
1996,
Pubmed
Kolega,
Phototoxicity and photoinactivation of blebbistatin in UV and visible light.
2004,
Pubmed
Kolega,
Cytoplasmic dynamics of myosin IIA and IIB: spatial 'sorting' of isoforms in locomoting cells.
1998,
Pubmed
Mandato,
Contraction and polymerization cooperate to assemble and close actomyosin rings around Xenopus oocyte wounds.
2001,
Pubmed
,
Xenbase
Mandato,
Actomyosin transports microtubules and microtubules control actomyosin recruitment during Xenopus oocyte wound healing.
2003,
Pubmed
,
Xenbase
Matsumura,
Regulation of myosin II during cytokinesis in higher eukaryotes.
2005,
Pubmed
May,
Phagocytosis and the actin cytoskeleton.
2001,
Pubmed
Merrifield,
Neural Wiskott Aldrich Syndrome Protein (N-WASP) and the Arp2/3 complex are recruited to sites of clathrin-mediated endocytosis in cultured fibroblasts.
2004,
Pubmed
Merrifield,
Endocytic vesicles move at the tips of actin tails in cultured mast cells.
1999,
Pubmed
Nemoto,
Stabilization of exocytosis by dynamic F-actin coating of zymogen granules in pancreatic acini.
2004,
Pubmed
Pelkmans,
Local actin polymerization and dynamin recruitment in SV40-induced internalization of caveolae.
2002,
Pubmed
Rogers,
Regulation of melanosome movement in the cell cycle by reversible association with myosin V.
1999,
Pubmed
,
Xenbase
Schietroma,
A role for myosin 1e in cortical granule exocytosis in Xenopus oocytes.
2007,
Pubmed
,
Xenbase
Sirotkin,
Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast.
2005,
Pubmed
Sokac,
Kiss-and-coat and compartment mixing: coupling exocytosis to signal generation and local actin assembly.
2006,
Pubmed
Sokac,
Myosin-1c couples assembling actin to membranes to drive compensatory endocytosis.
2006,
Pubmed
,
Xenbase
Sokac,
Cdc42-dependent actin polymerization during compensatory endocytosis in Xenopus eggs.
2003,
Pubmed
,
Xenbase
Sokac,
Regulation and expression of metazoan unconventional myosins.
2000,
Pubmed
Straight,
Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor.
2003,
Pubmed
Sun,
Endocytic internalization in budding yeast requires coordinated actin nucleation and myosin motor activity.
2006,
Pubmed
Tang,
Motor domain-dependent localization of myo1b (myr-1).
2001,
Pubmed
van Weeren,
Rab3D and actin reveal distinct lamellar body subpopulations in alveolar epithelial type II cells.
2004,
Pubmed
Verkhovsky,
Myosin II filament assemblies in the active lamella of fibroblasts: their morphogenesis and role in the formation of actin filament bundles.
1995,
Pubmed
Yu,
Control of local actin assembly by membrane fusion-dependent compartment mixing.
2007,
Pubmed
,
Xenbase
