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Chromosome Res
2007 Jan 01;158:985-1000. doi: 10.1007/s10577-007-1179-1.
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Localized co-transcriptional recruitment of the multifunctional RNA-binding protein CELF1 by lampbrush chromosome transcription units.
Morgan GT
.
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The highly-extended transcription units of lampbrush chromosomes (LBCs) offer unique opportunities to study the co-transcriptional events occurring on nascent transcripts. Using LBCs from amphibian oocytes, I investigated whether CELF1, an RNA binding protein involved in the regulation of alternative splicing, mRNA stability and translation, is localized to active transcription units. Antibodies raised against mammalian (CUG-BP1) and amphibian (EDEN-BP) CELF1 were used to immunostain LBC spreads prepared from several species, including Xenopus laevis and the axolotl Ambystoma mexicanum. Up to about 50 separate LBC loci were convincingly immunostained and it was clear that CELF1 was present in the nascent RNPs of lateral loops. Furthermore, myc-tagged CUG-BP1 expressed in microinjected axolotl oocytes was specifically targeted to nascent transcripts of loops that recruit endogenous CELF1. In many active transcription units CELF1 was distinctly localized, being first recruited by nascent transcripts only far downstream of the transcription start site and remaining associated until the end of transcription. Overall it appears possible that the multiple functions of CELF1 in regulating posttranscriptional gene expression could all be predetermined during transcription by virtue of a region-specific binding to the nascent transcripts of target genes.
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Aguilera,
Cotranscriptional mRNP assembly: from the DNA to the nuclear pore.
2005, Pubmed
Aguilera,
Cotranscriptional mRNP assembly: from the DNA to the nuclear pore.
2005,
Pubmed
Andrulis,
The RNA processing exosome is linked to elongating RNA polymerase II in Drosophila.
,
Pubmed
Barreau,
Mammalian CELF/Bruno-like RNA-binding proteins: molecular characteristics and biological functions.
2006,
Pubmed
Bellini,
A putative zinc-binding protein on lampbrush chromosome loops.
1993,
Pubmed
Bentley,
The mRNA assembly line: transcription and processing machines in the same factory.
2002,
Pubmed
Bentley,
Rules of engagement: co-transcriptional recruitment of pre-mRNA processing factors.
2005,
Pubmed
Callan,
Chromosomes and nucleoli of the axolotl, Ambystoma mexicanum.
1966,
Pubmed
Cooper,
A reversal of misfortune for myotonic dystrophy?
2006,
Pubmed
Daneholt,
Assembly and transport of a premessenger RNP particle.
2001,
Pubmed
Dansithong,
MBNL1 is the primary determinant of focus formation and aberrant insulin receptor splicing in DM1.
2005,
Pubmed
Dreyfuss,
hnRNP proteins and the biogenesis of mRNA.
1993,
Pubmed
Eckmann,
The RNA-editing enzyme ADAR1 is localized to the nascent ribonucleoprotein matrix on Xenopus lampbrush chromosomes but specifically associates with an atypical loop.
1999,
Pubmed
,
Xenbase
Fardaei,
In vivo co-localisation of MBNL protein with DMPK expanded-repeat transcripts.
2001,
Pubmed
Gall,
Assembly of lampbrush chromosomes from sperm chromatin.
1998,
Pubmed
,
Xenbase
Gall,
Structure in the amphibian germinal vesicle.
2004,
Pubmed
,
Xenbase
Gilbert,
Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation.
2004,
Pubmed
Good,
A family of human RNA-binding proteins related to the Drosophila Bruno translational regulator.
2000,
Pubmed
,
Xenbase
Jantsch,
Assembly and localization of the U1-specific snRNP C protein in the amphibian oocyte.
1992,
Pubmed
,
Xenbase
Kanadia,
Reversal of RNA missplicing and myotonia after muscleblind overexpression in a mouse poly(CUG) model for myotonic dystrophy.
2006,
Pubmed
Kloc,
RNA localization mechanisms in oocytes.
2005,
Pubmed
Kotovic,
Cotranscriptional recruitment of the U1 snRNP to intron-containing genes in yeast.
2003,
Pubmed
Krecic,
hnRNP complexes: composition, structure, and function.
1999,
Pubmed
Lacroix,
Monoclonal antibodies to lampbrush chromosome antigens of Pleurodeles waltlii.
1985,
Pubmed
Ladd,
The CELF family of RNA binding proteins is implicated in cell-specific and developmentally regulated alternative splicing.
2001,
Pubmed
Ling,
A sequence motif conserved in diverse nuclear proteins identifies a protein interaction domain utilised for nuclear targeting by human TFIIS.
2006,
Pubmed
Mahadevan,
Reversible model of RNA toxicity and cardiac conduction defects in myotonic dystrophy.
2006,
Pubmed
Misteli,
Protein dynamics: implications for nuclear architecture and gene expression.
2001,
Pubmed
Morgan,
RNA polymerase II in Cajal bodies of amphibian oocytes.
2000,
Pubmed
,
Xenbase
Morgan,
Lampbrush chromosomes and associated bodies: new insights into principles of nuclear structure and function.
2002,
Pubmed
Murphy,
RNA polymerase III in Cajal bodies and lampbrush chromosomes of the Xenopus oocyte nucleus.
2002,
Pubmed
,
Xenbase
Neugebauer,
On the importance of being co-transcriptional.
2002,
Pubmed
Osheim,
RNP particles at splice junction sequences on Drosophila chorion transcripts.
1985,
Pubmed
Paillard,
East of EDEN was a poly(A) tail.
2003,
Pubmed
,
Xenbase
Paillard,
EDEN and EDEN-BP, a cis element and an associated factor that mediate sequence-specific mRNA deadenylation in Xenopus embryos.
1998,
Pubmed
,
Xenbase
Piñol-Roma,
A novel heterogeneous nuclear RNP protein with a unique distribution on nascent transcripts.
1989,
Pubmed
Proudfoot,
Integrating mRNA processing with transcription.
2002,
Pubmed
Roth,
Targeting of a chromosomal protein to the nucleus and to lampbrush chromosome loops.
1989,
Pubmed
,
Xenbase
Roth,
Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes.
1987,
Pubmed
Savkur,
Aberrant regulation of insulin receptor alternative splicing is associated with insulin resistance in myotonic dystrophy.
2001,
Pubmed
Singh,
A specific SR protein binds preferentially to the secretory protein gene transcripts in salivary glands of Chironomus tentans.
2006,
Pubmed
Smillie,
RNA helicase p54 (DDX6) is a shuttling protein involved in nuclear assembly of stored mRNP particles.
2002,
Pubmed
,
Xenbase
Smith,
Subnuclear localization and Cajal body targeting of transcription elongation factor TFIIS in amphibian oocytes.
2003,
Pubmed
,
Xenbase
Solovei,
Single stranded nucleic acid binding structures on chicken lampbrush chromosomes.
1995,
Pubmed
Sun,
Conspicuous accumulation of transcription elongation repressor hrp130/CA150 on the intron-rich Balbiani ring 3 gene.
2004,
Pubmed
Timchenko,
Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy.
1996,
Pubmed
Timchenko,
RNA CUG repeats sequester CUGBP1 and alter protein levels and activity of CUGBP1.
2001,
Pubmed
Timchenko,
CUG repeat binding protein (CUGBP1) interacts with the 5' region of C/EBPbeta mRNA and regulates translation of C/EBPbeta isoforms.
1999,
Pubmed
Tuma,
Identification and characterization of a sphere organelle protein.
1993,
Pubmed
,
Xenbase
Warren,
Cytostellin: a novel, highly conserved protein that undergoes continuous redistribution during the cell cycle.
1992,
Pubmed
Wu,
Small nuclear ribonucleoproteins and heterogeneous nuclear ribonucleoproteins in the amphibian germinal vesicle: loops, spheres, and snurposomes.
1991,
Pubmed
,
Xenbase
