Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nature
2008 Jun 19;4537198:1132-6. doi: 10.1038/nature06923.
Show Gene links
Show Anatomy links
Midzone activation of aurora B in anaphase produces an intracellular phosphorylation gradient.
Fuller BG
,
Lampson MA
,
Foley EA
,
Rosasco-Nitcher S
,
Le KV
,
Tobelmann P
,
Brautigan DL
,
Stukenberg PT
,
Kapoor TM
.
???displayArticle.abstract???
Proper partitioning of the contents of a cell between two daughters requires integration of spatial and temporal cues. The anaphase array of microtubules that self-organize at the spindle midzone contributes to positioning the cell-division plane midway between the segregating chromosomes. How this signalling occurs over length scales of micrometres, from the midzone to the cell cortex, is not known. Here we examine the anaphase dynamics of protein phosphorylation by aurora B kinase, a key mitotic regulator, using fluorescence resonance energy transfer (FRET)-based sensors in living HeLa cells and immunofluorescence of native aurora B substrates. Quantitative analysis of phosphorylation dynamics, using chromosome- and centromere-targeted sensors, reveals that changes are due primarily to position along the division axis rather than time. These dynamics result in the formation of a spatial phosphorylation gradient early in anaphase that is centred at the spindle midzone. This gradient depends on aurora B targeting to a subpopulation of microtubules that activate it. Aurora kinase activity organizes the targeted microtubules to generate a structure-based feedback loop. We propose that feedback between aurora B kinase activation and midzone microtubules generates a gradient of post-translational marks that provides spatial information for events in anaphase and cytokinesis.
Alsop,
Microtubules continuously dictate distribution of actin filaments and positioning of cell cleavage in grasshopper spermatocytes.
2004, Pubmed
Alsop,
Microtubules continuously dictate distribution of actin filaments and positioning of cell cleavage in grasshopper spermatocytes.
2004,
Pubmed
Bement,
A microtubule-dependent zone of active RhoA during cleavage plane specification.
2005,
Pubmed
,
Xenbase
Bishop,
Phosphorylation of the carboxyl terminus of inner centromere protein (INCENP) by the Aurora B Kinase stimulates Aurora B kinase activity.
2002,
Pubmed
Canman,
Determining the position of the cell division plane.
2003,
Pubmed
Durocher,
The molecular basis of FHA domain:phosphopeptide binding specificity and implications for phospho-dependent signaling mechanisms.
2000,
Pubmed
Glotzer,
The molecular requirements for cytokinesis.
2005,
Pubmed
Gruneberg,
Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2.
2004,
Pubmed
Hauf,
The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint.
2003,
Pubmed
Kaláb,
Analysis of a RanGTP-regulated gradient in mitotic somatic cells.
2006,
Pubmed
,
Xenbase
Lan,
Aurora B phosphorylates centromeric MCAK and regulates its localization and microtubule depolymerization activity.
2004,
Pubmed
,
Xenbase
Meinhardt,
Pattern formation by local self-activation and lateral inhibition.
2000,
Pubmed
Murata-Hori,
Probing the dynamics and functions of aurora B kinase in living cells during mitosis and cytokinesis.
2002,
Pubmed
Neef,
Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis.
2003,
Pubmed
Nguyen,
Evolutionary optimization of fluorescent proteins for intracellular FRET.
2005,
Pubmed
Obenauer,
Scansite 2.0: Proteome-wide prediction of cell signaling interactions using short sequence motifs.
2003,
Pubmed
Peters,
Probing cell-division phenotype space and Polo-like kinase function using small molecules.
2006,
Pubmed
,
Xenbase
Rosasco-Nitcher,
Centromeric Aurora-B activation requires TD-60, microtubules, and substrate priming phosphorylation.
2008,
Pubmed
,
Xenbase
Ruchaud,
Chromosomal passengers: conducting cell division.
2007,
Pubmed
Shelby,
Dynamic elastic behavior of alpha-satellite DNA domains visualized in situ in living human cells.
1996,
Pubmed
Söderberg,
Direct observation of individual endogenous protein complexes in situ by proximity ligation.
2006,
Pubmed
Su,
Exit from mitosis in Drosophila syncytial embryos requires proteolysis and cyclin degradation, and is associated with localized dephosphorylation.
1998,
Pubmed
Violin,
A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C.
2003,
Pubmed
Wheatley,
CDK1 inactivation regulates anaphase spindle dynamics and cytokinesis in vivo.
1997,
Pubmed
Wheatley,
Midzone microtubule bundles are continuously required for cytokinesis in cultured epithelial cells.
1996,
Pubmed
Yasui,
Autophosphorylation of a newly identified site of Aurora-B is indispensable for cytokinesis.
2004,
Pubmed
Zeitlin,
Differential regulation of CENP-A and histone H3 phosphorylation in G2/M.
2001,
Pubmed
