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Onecut genes belong to a family of transcription factors that are known to be important in embryonic development. In the present study, we analyzed the pattern of expression of Onecut-1/HNF6 in Xenopus tropicalis using RT-PCR and whole mount in situ hybridization. Expression of the Xenopus tropicalis Onecut-1/HNF6 gene was found to be conserved in the neural tube, the sensory placodes and in the anteriorventral endoderm in a domain consistent with the developing liver primordium.
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19123138
???displayArticle.link???Int J Dev Biol ???displayArticle.grants???[+]
Fig. 1. Genomic structure and temporal expression of X. tropicalis HNF6. (A) The genomic structure of HNF6. The boxes represent exons and the line between them intron sequence. The sizes of the coding regions of the exons are given. Intron sizes are also given. Exon 1contains the 5UTR and the transla- tion start site. Exon 3 contains the stop site and the 3UTR sequence.
Fig. 1. Genomic structure and temporal expression of X. tropicalis HNF6. (B) HNF6 RT-PCR of staged embryos using forward primer situated in exon 1 and reverse primer in exon 3. Two bands are visible. Sequencing of PCR products indicates that the smaller band does not contain exon 2 (data not shown). RT-PCR was also used to detect the housekeeping gene ODC.
Fig. 1. Genomic structure and temporal expression of X. tropicalis HNF6. (C,D,E,F), In situ hybridisation of whole X. tropicalis embryos, with (C) showing view from dorsal aspect and (D,E,F) from the lateral aspect. Key: AVE, anteriorventralendoderm; L, liver; NC, neural crest; NT, neural tube; SP, sensory placodes.
Fig. 2. Spatial expression of X.tropicalis HNF6. Neural expression of HNF6 (A-G) and endoderm expression (H,J). Embryos at st. 17 (A,B), st. 22 (C), st. 24 (D,E), st. 25 (F,H,I,J), st. 32 (G) are shown. The embryo in (A) was bisected along the midline, the anterior region is identified by the presence the pigmented cement gland (Ce). The region highlighted by the rectangular box is shown at higher magnification in (B). (C,F,G) Expression in the cranial region. (D,E) Coronal sections. (H) St. 25 embryo, solid line showing the plane of section of wax section (I) and bisected embryo (J). Abbreviations: A, anterior; AD, anterodorsal lateral line placode; AVE, anteriorventralendoderm; Ce, cement gland; D, dorsal; E, eye; F, foregut; M, middle lateral line placode; N, notochord; NT, neural tube; P, posterior; PV, trigeminal placode; Po, posteriorlateral line placode; SP, sensory placodes; V, ventral.
onecut1 (one cut homeobox 1 )gene expression in Xenopus tropicalis embryos, NF stage 19, as assayed by in situ hybridization. Lateral view: anteriorright, dorsal up.
