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Plant Physiol
2004 Mar 01;1343:969-78. doi: 10.1104/pp.103.032102.
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A nodule-specific dicarboxylate transporter from alder is a member of the peptide transporter family.
Jeong J
,
Suh S
,
Guan C
,
Tsay YF
,
Moran N
,
Oh CJ
,
An CS
,
Demchenko KN
,
Pawlowski K
,
Lee Y
.
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Alder (Alnus glutinosa) and more than 200 angiosperms that encompass 24 genera are collectively called actinorhizal plants. These plants form a symbiotic relationship with the nitrogen-fixing actinomycete Frankia strain HFPArI3. The plants provide the bacteria with carbon sources in exchange for fixed nitrogen, but this metabolite exchange in actinorhizal nodules has not been well defined. We isolated an alder cDNA from a nodule cDNA library by differential screening with nodule versus root cDNA and found that it encoded a transporter of the PTR (peptide transporter) family, AgDCAT1. AgDCAT1 mRNA was detected only in the nodules and not in other plant organs. Immunolocalization analysis showed that AgDCAT1 protein is localized at the symbiotic interface. The AgDCAT1 substrate was determined by its heterologous expression in two systems. Xenopus laevis oocytes injected with AgDCAT1 cRNA showed an outward current when perfused with malate or succinate, and AgDCAT1 was able to complement a dicarboxylate uptake-deficient Escherichia coli mutant. Using the E. coli system, AgDCAT1 was shown to be a dicarboxylate transporter with a K(m) of 70 microm for malate. It also transported succinate, fumarate, and oxaloacetate. To our knowledge, AgDCAT1 is the first dicarboxylate transporter to be isolated from the nodules of symbiotic plants, and we suggest that it may supply the intracellular bacteria with dicarboxylates as carbon sources.
Ahmed,
The use of valinomycin, nigericin and trichlorocarbanilide in control of the protonmotive force in Escherichia coli cells.
1983, Pubmed
Ahmed,
The use of valinomycin, nigericin and trichlorocarbanilide in control of the protonmotive force in Escherichia coli cells.
1983,
Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Arima,
Glucocorticoid regulation and glycosylation of mouse intestinal type IIb Na-P(i) cotransporter during ontogeny.
2002,
Pubmed
Brosius,
Regulation of ribosomal RNA promoters with a synthetic lac operator.
1984,
Pubmed
Camarasa,
Characterization of Schizosaccharomyces pombe malate permease by expression in Saccharomyces cerevisiae.
2001,
Pubmed
Dean,
Purification and functional reconstitution of soybean nodulin 26. An aquaporin with water and glycerol transport properties.
1999,
Pubmed
,
Xenbase
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Fiermonte,
Organization and sequence of the gene for the human mitochondrial dicarboxylate carrier: evolution of the carrier family.
1999,
Pubmed
Fischer,
The activity of the ATP synthase from Escherichia coli is regulated by the transmembrane proton motive force.
2000,
Pubmed
Frommer,
Cloning of an Arabidopsis histidine transporting protein related to nitrate and peptide transporters.
1994,
Pubmed
Grobler,
The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids.
1995,
Pubmed
Husain,
Affinity for the nuclear compartment and expression during cell differentiation implicate phosphorylated Groucho/TLE1 forms of higher molecular mass in nuclear functions.
1996,
Pubmed
Huss-Danell,
Actinorhizal symbioses and their N2 fixation.
1997,
Pubmed
Janausch,
C4-dicarboxylate carriers and sensors in bacteria.
2002,
Pubmed
Kim,
AtKUP1: an Arabidopsis gene encoding high-affinity potassium transport activity.
1998,
Pubmed
,
Xenbase
Kouchi,
Isolation and characterization of novel nodulin cDNAs representing genes expressed at early stages of soybean nodule development.
1993,
Pubmed
Labarre,
Cloning and characterization of the genes encoding the malolactic enzyme and the malate permease of Leuconostoc oenos.
1996,
Pubmed
Lee,
Signal-dependent phosphorylation of the membrane-bound NarX two-component sensor-transmitter protein of Escherichia coli: nitrate elicits a superior anion ligand response compared to nitrite.
1999,
Pubmed
Li,
Functional characterization of CitM, the Mg2+-citrate transporter.
2002,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Lin,
Single-channel currents produced by the serotonin transporter and analysis of a mutation affecting ion permeation.
1996,
Pubmed
,
Xenbase
Lo,
Transport of succinate in Escherichia coli. I. Biochemical and genetic studies of transport in whole cells.
1972,
Pubmed
Martinoia,
Functional reconstitution of the malate carrier of barley mesophyll vacuoles in liposomes.
1991,
Pubmed
Minet,
Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs.
1992,
Pubmed
Moreau,
GmZIP1 encodes a symbiosis-specific zinc transporter in soybean.
2002,
Pubmed
Mylona,
Symbiotic Nitrogen Fixation.
1995,
Pubmed
Ouyang,
Protein phosphorylation stimulates the rate of malate uptake across the peribacteroid membrane of soybean nodules.
1991,
Pubmed
Ou Yang,
Specificity and regulation of the dicarboxylate carrier on the peribacteroid membrane of soybean nodules.
1990,
Pubmed
Palmieri,
Yeast mitochondrial carriers: bacterial expression, biochemical identification and metabolic significance.
2000,
Pubmed
Pawlowski,
Rhizobial and Actinorhizal Symbioses: What Are the Shared Features?
1996,
Pubmed
Picault,
Identification of a novel transporter for dicarboxylates and tricarboxylates in plant mitochondria. Bacterial expression, reconstitution, functional characterization, and tissue distribution.
2002,
Pubmed
Ribeiro,
A nodule-specific gene encoding a subtilisin-like protease is expressed in early stages of actinorhizal nodule development.
1995,
Pubmed
Rivers,
Functional analysis of nodulin 26, an aquaporin in soybean root nodule symbiosomes.
1997,
Pubmed
,
Xenbase
Schiestl,
High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.
1989,
Pubmed
Schwartz,
Acid incubation reverses the polarity of intercalated cell transporters, an effect mediated by hensin.
2002,
Pubmed
Sensi,
Measurement of intracellular free zinc in living cortical neurons: routes of entry.
1997,
Pubmed
Sonders,
Multiple ionic conductances of the human dopamine transporter: the actions of dopamine and psychostimulants.
1997,
Pubmed
,
Xenbase
Sonders,
Channels in transporters.
1996,
Pubmed
Stadler,
Subcellular localization of the inducible Chlorella HUP1 monosaccharide-H+ symporter and cloning of a Co-induced galactose-H+ symporter.
1995,
Pubmed
Steiner,
The PTR family: a new group of peptide transporters.
1995,
Pubmed
Tsay,
The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate-inducible nitrate transporter.
1993,
Pubmed
,
Xenbase
Uozumi,
Escherichia coli as an expression system for K(+) transport systems from plants.
2001,
Pubmed
Verma,
Signals in Root Nodule Organogenesis and Endocytosis of Rhizobium.
1992,
Pubmed
Williams,
TRANSPORTERS RESPONSIBLE FOR THE UPTAKE AND PARTITIONING OF NITROGENOUS SOLUTES.
2001,
Pubmed
Yu,
Na(+)-Ca2+ exchange currents in cortical neurons: concomitant forward and reverse operation and effect of glutamate.
1997,
Pubmed
