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Complex relationship between TCTP, microtubules and actin microfilaments regulates cell shape in normal and cancer cells.
Bazile F
,
Pascal A
,
Arnal I
,
Le Clainche C
,
Chesnel F
,
Kubiak JZ
.
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Translationally controlled tumor-associated protein (TCTP) is a ubiquitous and highly conserved protein implicated in cancers. Here, we demonstrate that interactions of TCTP with microtubules (MTs) are functionally important but indirect, and we reveal novel interaction of TCTP with the actin cytoskeleton. Firstly, immunofluorescence in Xenopus XL2 cells revealed cytoplasmic fibers stained with TCTP but not with tubulin antibodies, as well as MTs free of TCTP. Furthermore, TCTP localized to a subset of actin-rich fibers in migrating cells. Secondly, Xenopus laevis TCTP did not affect in vitro assembly/disassembly of MTs and lacked MT-binding affinity both in pull-down assays and in cell-free extracts. Although TCTP also failed to bind to purified filamentous actin (F-actin), it was associated with microfilaments in cell-free extracts. Thirdly, TCTP concentrated in mitotic spindle did not colocalize with MTs and was easily dissociated from these structures except at the poles. Finally, RNA interference knockdown of TCTP in XL2 and HeLa cells provoked drastic, MT-dependent shape change. These data show that although TCTP interacts with MTs, it does not behave as classic MT-associated protein. Our evidence for an association of TCTP with F-actin structures, and for an involvement in cell shape regulation, implicates this protein in integrating cytoskeletal interactions both in interphase and mitosis providing a new avenue to fully understand the role of TCTP.
Applewhite,
Ena/VASP proteins have an anti-capping independent function in filopodia formation.
2007, Pubmed
Applewhite,
Ena/VASP proteins have an anti-capping independent function in filopodia formation.
2007,
Pubmed
Arcuri,
The translationally controlled tumor protein is a novel calcium binding protein of the human placenta and regulates calcium handling in trophoblast cells.
2005,
Pubmed
Arcuri,
Translationally controlled tumor protein (TCTP) in the human prostate and prostate cancer cells: expression, distribution, and calcium binding activity.
2004,
Pubmed
Arnal,
CLIP-170/tubulin-curved oligomers coassemble at microtubule ends and promote rescues.
2004,
Pubmed
Basu,
Shaping the actin cytoskeleton using microtubule tips.
2007,
Pubmed
Bommer,
The translationally controlled tumour protein (TCTP).
2004,
Pubmed
Burgess,
Chfr interacts and colocalizes with TCTP to the mitotic spindle.
2008,
Pubmed
,
Xenbase
Chen,
A knockout mouse approach reveals that TCTP functions as an essential factor for cell proliferation and survival in a tissue- or cell type-specific manner.
2007,
Pubmed
Chitpatima,
Nucleotide sequence of a major messenger RNA for a 21 kilodalton polypeptide that is under translational control in mouse tumor cells.
1988,
Pubmed
de Pennart,
Microtubule tracks can be detected in mouse oocytes with an antibody directed against a calcium transporter.
1994,
Pubmed
Gachet,
The growth-related, translationally controlled protein P23 has properties of a tubulin binding protein and associates transiently with microtubules during the cell cycle.
1999,
Pubmed
González,
The interaction of Mip-90 with microtubules and actin filaments in human fibroblasts.
1998,
Pubmed
Guillaume,
Cellular distribution of translationally controlled tumor protein in rat and human testes.
2001,
Pubmed
Halpain,
The MAP1 family of microtubule-associated proteins.
2006,
Pubmed
Hsu,
Drosophila TCTP is essential for growth and proliferation through regulation of dRheb GTPase.
2007,
Pubmed
Kim,
Identification of the calcium binding sites in translationally controlled tumor protein.
2000,
Pubmed
Koziol,
Tpt1 activates transcription of oct4 and nanog in transplanted somatic nuclei.
2007,
Pubmed
,
Xenbase
Krueger,
A dynamin-cortactin-Arp2/3 complex mediates actin reorganization in growth factor-stimulated cells.
2003,
Pubmed
Kurisu,
Rac-WAVE2 signaling is involved in the invasive and metastatic phenotypes of murine melanoma cells.
2005,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Langdon,
Identification of the interaction between the human recombinant histamine releasing factor/translationally controlled tumor protein and elongation factor-1 delta (also known as eElongation factor-1B beta).
2004,
Pubmed
Laurent,
Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes.
1999,
Pubmed
Li,
Characterization of fortilin, a novel antiapoptotic protein.
2001,
Pubmed
Liu,
Stabilization and enhancement of the antiapoptotic activity of mcl-1 by TCTP.
2005,
Pubmed
Miyara,
Non-equivalence of embryonic and somatic cell nuclei affecting spindle composition in clones.
2006,
Pubmed
Murray,
Cell cycle extracts.
1991,
Pubmed
Noiges,
Microtubule-associated protein 1A (MAP1A) and MAP1B: light chains determine distinct functional properties.
2002,
Pubmed
Pierre,
CLIP-170 links endocytic vesicles to microtubules.
1992,
Pubmed
Rinnerthaler,
MMI1 (YKL056c, TMA19), the yeast orthologue of the translationally controlled tumor protein (TCTP) has apoptotic functions and interacts with both microtubules and mitochondria.
2006,
Pubmed
Sawin,
Mitotic spindle organization by a plus-end-directed microtubule motor.
1992,
Pubmed
,
Xenbase
Scheel,
Purification and analysis of authentic CLIP-170 and recombinant fragments.
1999,
Pubmed
Stierum,
Proteome analysis reveals novel proteins associated with proliferation and differentiation of the colorectal cancer cell line Caco-2.
2003,
Pubmed
Tani,
Bovine oocytes with the potential to reprogram somatic cell nuclei have a unique 23-kDa protein, phosphorylated transcriptionally controlled tumor protein (TCTP).
2007,
Pubmed
Thaw,
Structure of TCTP reveals unexpected relationship with guanine nucleotide-free chaperones.
2001,
Pubmed
Théry,
Cell shape and cell division.
2006,
Pubmed
Théry,
Experimental and theoretical study of mitotic spindle orientation.
2007,
Pubmed
Tsai,
A mitotic lamin B matrix induced by RanGTP required for spindle assembly.
2006,
Pubmed
,
Xenbase
Tuynder,
Biological models and genes of tumor reversion: cellular reprogramming through tpt1/TCTP and SIAH-1.
2002,
Pubmed
Tuynder,
Translationally controlled tumor protein is a target of tumor reversion.
2004,
Pubmed
Vitre,
EB1 regulates microtubule dynamics and tubulin sheet closure in vitro.
2008,
Pubmed
Yang,
An N-terminal region of translationally controlled tumor protein is required for its antiapoptotic activity.
2005,
Pubmed
Yarm,
Plk phosphorylation regulates the microtubule-stabilizing protein TCTP.
2002,
Pubmed
Yenofsky,
Regulation of mRNA utilization in mouse erythroleukemia cells induced to differentiate by exposure to dimethyl sulfoxide.
1983,
Pubmed
Yoon,
Identification of the self-interaction of rat TCTP/IgE-dependent histamine-releasing factor using yeast two-hybrid system.
2000,
Pubmed
Zheng,
The mitotic spindle matrix: a fibro-membranous lamin connection.
2006,
Pubmed
