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J Am Soc Nephrol
2009 Jul 01;207:1480-90. doi: 10.1681/ASN.2008070686.
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Nucleotides downregulate aquaporin 2 via activation of apical P2 receptors.
Wildman SS
,
Boone M
,
Peppiatt-Wildman CM
,
Contreras-Sanz A
,
King BF
,
Shirley DG
,
Deen PM
,
Unwin RJ
.
???displayArticle.abstract??? Vasopressin regulates water reabsorption in the collecting duct, but extracellular nucleotides modulate this regulation through incompletely understood mechanisms. We investigated these mechanisms using immortalized mouse collecting duct (mpkCCD) cells. Basolateral exposure to dDAVP induced AQP2 localization to the apical membrane, but co-treatment with ATP internalized AQP2. Because plasma membrane-bound P2 receptors (P2R) mediate the effects of extracellular nucleotides, we examined the abundance and localization of P2R in mpkCCD cells. In the absence of dDAVP, P2Y(1) and P2Y(4) receptors localized to the apical membrane, whereas P2X(2), P2X(4), P2X(5), P2X(7), P2Y(2), P2Y(11), and P2Y(12) receptors localized to the cytoplasm. dDAVP induced gene expression of P2X(1), which localized to the apical domain, and led to translocation of P2X(2) and P2Y(2) to the apical and basolateral membranes, respectively. In co-expression experiments, P2R activation decreased membrane AQP2 and AQP2-mediated water permeability in Xenopus oocytes expressing P2X(2), P2Y(2,) or P2Y(4) receptors, but not in oocytes expressing other P2R subtypes. In summary, these data suggest that AQP2-mediated water transport is downregulated not only by basolateral nucleotides, mediated by P2Y(2) receptors, but also by luminal nucleotides, mediated by P2X(2) and/or P2Y(4) receptors.
Bailey,
Axial distribution and characterization of basolateral P2Y receptors along the rat renal tubule.
2000, Pubmed
Bailey,
Axial distribution and characterization of basolateral P2Y receptors along the rat renal tubule.
2000,
Pubmed
Brown,
The ins and outs of aquaporin-2 trafficking.
2003,
Pubmed
Deen,
Water channels encoded by mutant aquaporin-2 genes in nephrogenic diabetes insipidus are impaired in their cellular routing.
1995,
Pubmed
,
Xenbase
Deen,
Urinary content of aquaporin 1 and 2 in nephrogenic diabetes insipidus.
1996,
Pubmed
,
Xenbase
Deen,
Requirement of human renal water channel aquaporin-2 for vasopressin-dependent concentration of urine.
1994,
Pubmed
,
Xenbase
Deen,
Aquaporin-2: COOH terminus is necessary but not sufficient for routing to the apical membrane.
2002,
Pubmed
Edwards,
Adenosine A1 receptor-mediated inhibition of vasopressin action in inner medullary collecting duct.
1994,
Pubmed
Edwards,
Basolateral, but not apical, ATP inhibits vasopressin action in rat inner medullary collecting duct.
2002,
Pubmed
Hasler,
Long term regulation of aquaporin-2 expression in vasopressin-responsive renal collecting duct principal cells.
2002,
Pubmed
Kamsteeg,
Short-chain ubiquitination mediates the regulated endocytosis of the aquaporin-2 water channel.
2006,
Pubmed
Kishore,
P2Y(2) receptors and water transport in the kidney.
2009,
Pubmed
Kishore,
Cellular localization of P2Y(2) purinoceptor in rat renal inner medulla and lung.
2000,
Pubmed
Kishore,
Extracellular nucleotide receptor inhibits AVP-stimulated water permeability in inner medullary collecting duct.
1995,
Pubmed
Kishore,
P2Y2 receptor mRNA and protein expression is altered in inner medullas of hydrated and dehydrated rats: relevance to AVP-independent regulation of IMCD function.
2005,
Pubmed
Kohan,
Autocrine role of endothelin in rat IMCD: inhibition of AVP-induced cAMP accumulation.
1993,
Pubmed
Lehrmann,
Luminal P2Y2 receptor-mediated inhibition of Na+ absorption in isolated perfused mouse CCD.
2002,
Pubmed
Leipziger,
Control of epithelial transport via luminal P2 receptors.
2003,
Pubmed
Li,
Development of lithium-induced nephrogenic diabetes insipidus is dissociated from adenylyl cyclase activity.
2006,
Pubmed
Marples,
Lithium-induced downregulation of aquaporin-2 water channel expression in rat kidney medulla.
1995,
Pubmed
Matsumura,
Transcriptional regulation of aquaporin-2 water channel gene by cAMP.
1997,
Pubmed
McCoy,
Nucleotides regulate NaCl transport in mIMCD-K2 cells via P2X and P2Y purinergic receptors.
1999,
Pubmed
Nadler,
PGE2 inhibits water permeability at a post-cAMP site in rat terminal inner medullary collecting duct.
1992,
Pubmed
Nejsum,
Bidirectional regulation of AQP2 trafficking and recycling: involvement of AQP2-S256 phosphorylation.
2005,
Pubmed
Nielsen,
Congestive heart failure in rats is associated with increased expression and targeting of aquaporin-2 water channel in collecting duct.
1997,
Pubmed
Nielsen,
Vasopressin increases water permeability of kidney collecting duct by inducing translocation of aquaporin-CD water channels to plasma membrane.
1995,
Pubmed
Rieg,
Mice lacking P2Y2 receptors have salt-resistant hypertension and facilitated renal Na+ and water reabsorption.
2007,
Pubmed
Roman,
Prostaglandin E2 and F2 alpha reduces urea reabsorption from the rat collecting duct.
1981,
Pubmed
Rouch,
Role of PGE(2) in alpha(2)-induced inhibition of AVP- and cAMP-stimulated H(2)O, Na(+), and urea transport in rat IMCD.
2000,
Pubmed
Rouse,
ATP inhibits the hydrosmotic effect of AVP in rabbit CCT: evidence for a nucleotide P2u receptor.
1994,
Pubmed
Schafer,
Low Na+ diet inhibits Na+ and water transport response to vasopressin in rat cortical collecting duct.
1998,
Pubmed
Sun,
Chronic dDAVP infusion in rats decreases the expression of P2Y2 receptor in inner medulla and P2Y2 receptor-mediated PGE2 release by IMCD.
2005,
Pubmed
Takata,
Molecular mechanisms and drug development in aquaporin water channel diseases: water channel aquaporin-2 of kidney collecting duct cells.
2004,
Pubmed
Taylor,
Epithelial P2X purinergic receptor channel expression and function.
1999,
Pubmed
Thomas,
P2Y(2) receptor-mediated inhibition of amiloride-sensitive short circuit current in M-1 mouse cortical collecting duct cells.
2001,
Pubmed
Turner,
The pattern of distribution of selected ATP-sensitive P2 receptor subtypes in normal rat kidney: an immunohistological study.
2003,
Pubmed
Unwin,
Purinergic signaling along the renal tubule: the current state of play.
2003,
Pubmed
Vallon,
P2 receptors in the regulation of renal transport mechanisms.
2008,
Pubmed
van Balkom,
The role of putative phosphorylation sites in the targeting and shuttling of the aquaporin-2 water channel.
2002,
Pubmed
Vekaria,
Intraluminal ATP concentrations in rat renal tubules.
2006,
Pubmed
Welch,
P2Y2 receptor-stimulated release of prostaglandin E2 by rat inner medullary collecting duct preparations.
2003,
Pubmed
Wildman,
Regulatory interdependence of cloned epithelial Na+ channels and P2X receptors.
2005,
Pubmed
,
Xenbase
Wildman,
Sodium-dependent regulation of renal amiloride-sensitive currents by apical P2 receptors.
2008,
Pubmed
Wildman,
P2X receptors: epithelial ion channels and regulators of salt and water transport.
2008,
Pubmed
Xia,
Extracellular ATP-induced calcium signaling in mIMCD-3 cells requires both P2X and P2Y purinoceptors.
2004,
Pubmed
Xu,
Upregulation of aquaporin-2 water channel expression in chronic heart failure rat.
1997,
Pubmed
Yasui,
Adenylate cyclase-coupled vasopressin receptor activates AQP2 promoter via a dual effect on CRE and AP1 elements.
1997,
Pubmed
