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Fig. 3. Riboprobe wholemount in situ hybridization in Xenopus embryos. CAP2 is expressed along the paraxial mesoderm and in the region where the stomadeal pocket and cement glad will form (A). Subsequently, the paraxial mesoderm begins to segment (B) and cranio- facial expression drops off as somites differentiate (C). There is a brief period of expression in lens (white arrow) at stage 27, and in the heart (H) primordia (D). By stage 27, heart (H) expression is well established, and remains breifly de- tectable in the lens (L) (E). In contrast, at these stages CAP1 is ex- pressed in periocular mesencyme, lens, sensorial ectoderm and branchial arch (D insert). At later stages, CAP2 is expressed in the precursors to striated muscle including the myotome (Myo) (F), and in the developing heart (H), otic vesicle (O), musculus intermandibularis (IM), musculus orbitohyoidis (OH), and musculus subacuales rectis (SR) (G). At this stage, CAP2 is expressed in the retina, but CAP1 is not, although there is an annular domain of CAP1 expression around the circumference of the developing lens (G left insert). CAP1 is expressed in branchial arches but not in retina (compare G with CAP1 right insert). Expression seems to diminish when the muscles differentiate to the point of displaying visible striations (compare OH and Heart in G vs. H). Embryonic stages are indicated in the lower left corner of each photograph, and bars with numbers represent scale in micrometers.
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Fig. 4. Expression of CAP2 in sectioned embryos. The anterior end of the embryo displays staining in the region that ultimately generates the cement gland (bracket) (A). At later stages, expression is up- regulated in presumptive heart (B), and expresses predominantly in the mesodermally derived tissues of this organ when it forms a tube (C). CAP2 expressing myotome migrates ventrally and around the flank from the somites (D).
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Fig. 5. Expression of CAP2 in sectioned muscle and eye. CAP2 is also expressed in the myoblasts as they aggregate and fuse into tubes (arrows) (A), but transcription begins to diminish when the muscle fibers have differentiated sufficiently to show striations (compare relatively undifferentiated dark arrow with striated white arrows (B). Expression in lens (L) is evident in the domain of forming lens fibers (see arrow in (C)), but it disappears rapidly thereafter whereupon the outer and inner plexiform layers (OPL and IPL, respectively) of the retina, as well as the chorion (C) transcribe the gene (D).
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cap2 (CAP, adenylate cyclase-associated protein, 2) gene expression in Xenopus laevis embryos, NF stage 21, as assayed by in situ hybridization, lateral view, dorsal up, anterior left.
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cap2 (CAP, adenylate cyclase-associated protein, 2) gene expression in Xenopus laevis embryos, NF stage 24, as assayed by in situ hybridization, lateral view, dorsal up, anterior left.
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cap2 (CAP, adenylate cyclase-associated protein, 2) gene expression in Xenopus laevis embryos, NF stage 27, as assayed by in situ hybridization, lateral view, dorsal up, anterior left.
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cap2 (CAP, adenylate cyclase-associated protein, 2) gene expression in Xenopus laevis embryos, NF stage 40, trunk region, as assayed by in situ hybridization, lateral view, dorsal up, anterior left.
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cap (cyclase associated actin cytoskeleton regulatory protein 2) gene expression in Xenopus laevis embryos, NF stage 20, as assayed by in situ hybridization, anterior view, dorsal up.
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cap1 (CAP, adenylate cyclase-associated protein, 1) gene expression in Xenopus laevis embryos, NF stage 27, as assayed by in situ hybridization, lateral view, dorsal up, anterior left.
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cap2 (CAP, adenylate cyclase-associated protein, 2) gene expression in Xenopus laevis embryo, head region, NF stage 40, as assayed by in situ hybridization, lateral view, dorsal up, anterior left.
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