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Synaptic maturation of the Xenopus retinotectal system: effects of brain-derived neurotrophic factor on synapse ultrastructure.
Nikolakopoulou AM
,
Meynard MM
,
Marshak S
,
Cohen-Cory S
.
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Synaptogenesis is a dynamic process that involves structural changes in developing axons and dendrites as synapses form and mature. The visual system of Xenopus laevis has been used as a model to study dynamic changes in axons and dendrites as synapses form in the living brain and the molecular mechanisms that control these processes. Brain-derived neurotrophic factor (BDNF) contributes to the establishment and refinement of visual connectivity by modulating retinal ganglion cell (RGC) axon arborization and presynaptic differentiation. Here, we have analyzed the ultrastructural organization of the Xenopus retinotectal system to understand better the maturation of this synaptic circuit and the relation between synapse ultrastructure and the structural changes in connectivity that take place in response to BDNF. Expression of yellow fluorescent protein (YFP) followed by preembedding immunoelectron microscopy was used to identify RGC axons specifically in living tadpoles. Injection of recombinant BDNF was used to alter endogenous BDNF levels acutely in the optic tectum. Our studies reveal a rapid transition from a relatively immature synaptic circuit in which retinotectal synapses are formed on developing filopodial-like processes to a circuit in which RGCaxon terminals establish synapses with dendritic shafts and spines. Moreover, our studies reveal that BDNF treatment increases the number of spine synapses and docked vesicle number at YFP-identified synaptic sites within 24 hours of treatment. These fine structural changes at retinotectal synapses are consistent with the role that BDNF plays in the functional maturation of synaptic circuits and with dynamic, rapid changes in synaptic connectivity during development.
Alonso,
ERK1/2 activation is necessary for BDNF to increase dendritic spine density in hippocampal CA1 pyramidal neurons.
2004, Pubmed
Alonso,
ERK1/2 activation is necessary for BDNF to increase dendritic spine density in hippocampal CA1 pyramidal neurons.
2004,
Pubmed
Alsina,
Visualizing synapse formation in arborizing optic axons in vivo: dynamics and modulation by BDNF.
2001,
Pubmed
,
Xenbase
Amaral,
Transient receptor potential channels as novel effectors of brain-derived neurotrophic factor signaling: potential implications for Rett syndrome.
2007,
Pubmed
Aoki,
Localization of brain-derived neurotrophic factor and TrkB receptors to postsynaptic densities of adult rat cerebral cortex.
2000,
Pubmed
Bramham,
Local protein synthesis, actin dynamics, and LTP consolidation.
2008,
Pubmed
Calverley,
Contributions of dendritic spines and perforated synapses to synaptic plasticity.
1990,
Pubmed
Cline,
Dendritic arbor development and synaptogenesis.
2001,
Pubmed
Cohen-Cory,
Effects of brain-derived neurotrophic factor on optic axon branching and remodelling in vivo.
1995,
Pubmed
,
Xenbase
Cohen-Cory,
The developing synapse: construction and modulation of synaptic structures and circuits.
2002,
Pubmed
Cohen-Cory,
The cellular patterns of BDNF and trkB expression suggest multiple roles for BDNF during Xenopus visual system development.
1996,
Pubmed
,
Xenbase
Cohen-Cory,
Neurotrophic regulation of retinal ganglion cell synaptic connectivity: from axons and dendrites to synapses.
2004,
Pubmed
,
Xenbase
Drake,
Ultrastructural localization of full-length trkB immunoreactivity in rat hippocampus suggests multiple roles in modulating activity-dependent synaptic plasticity.
1999,
Pubmed
Du,
Rapid BDNF-induced retrograde synaptic modification in a developing retinotectal system.
2004,
Pubmed
,
Xenbase
Elmariah,
Postsynaptic TrkB-mediated signaling modulates excitatory and inhibitory neurotransmitter receptor clustering at hippocampal synapses.
2004,
Pubmed
Fiala,
Synaptogenesis via dendritic filopodia in developing hippocampal area CA1.
1998,
Pubmed
Gomes,
The dynamic distribution of TrkB receptors before, during, and after synapse formation between cortical neurons.
2006,
Pubmed
Gravagna,
Localization of Kv2.2 protein in Xenopus laevis embryos and tadpoles.
2008,
Pubmed
,
Xenbase
Harris,
Variation in the number, location and size of synaptic vesicles provides an anatomical basis for the nonuniform probability of release at hippocampal CA1 synapses.
1995,
Pubmed
Hocking,
TGFbeta ligands promote the initiation of retinal ganglion cell dendrites in vitro and in vivo.
2008,
Pubmed
,
Xenbase
Horch,
Destabilization of cortical dendrites and spines by BDNF.
1999,
Pubmed
Hu,
BDNF stabilizes synapses and maintains the structural complexity of optic axons in vivo.
2005,
Pubmed
,
Xenbase
Hua,
Neural activity and the dynamics of central nervous system development.
2004,
Pubmed
Iwakura,
Dopamine D1 receptor-induced signaling through TrkB receptors in striatal neurons.
2008,
Pubmed
Jontes,
Filopodia, spines, and the generation of synaptic diversity.
2000,
Pubmed
Jontes,
Growth cone and dendrite dynamics in zebrafish embryos: early events in synaptogenesis imaged in vivo.
2000,
Pubmed
Lin,
Mechanisms of synaptic vesicle exocytosis.
2000,
Pubmed
Lohmann,
A role for local calcium signaling in rapid synaptic partner selection by dendritic filopodia.
2008,
Pubmed
Luikart,
Receptor tyrosine kinase B-mediated excitatory synaptogenesis.
2006,
Pubmed
Manitt,
Netrin participates in the development of retinotectal synaptic connectivity by modulating axon arborization and synapse formation in the developing brain.
2009,
Pubmed
,
Xenbase
Markham,
Actin filament organization within dendrites and dendritic spines during development.
1986,
Pubmed
Marshak,
Cell-autonomous TrkB signaling in presynaptic retinal ganglion cells mediates axon arbor growth and synapse maturation during the establishment of retinotectal synaptic connectivity.
2007,
Pubmed
,
Xenbase
Martínez,
TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connections.
1998,
Pubmed
Meyer,
Evidence from in vivo imaging that synaptogenesis guides the growth and branching of axonal arbors by two distinct mechanisms.
2006,
Pubmed
Mumm,
In vivo imaging reveals dendritic targeting of laminated afferents by zebrafish retinal ganglion cells.
2006,
Pubmed
Nägerl,
Protracted synaptogenesis after activity-dependent spinogenesis in hippocampal neurons.
2007,
Pubmed
Niell,
In vivo imaging of synapse formation on a growing dendritic arbor.
2004,
Pubmed
Norden,
Dynamics of retinotectal synaptogenesis in normal and 3-eyed frogs: evidence for the postsynaptic regulation of synapse number.
1994,
Pubmed
O'Rourke,
Dynamic changes in optic fiber terminal arbors lead to retinotopic map formation: an in vivo confocal microscopic study.
1990,
Pubmed
,
Xenbase
Otal,
Lack of TrkB and TrkC signaling alters the synaptogenesis and maturation of mossy fiber terminals in the hippocampus.
2005,
Pubmed
Pinches,
Distribution of synaptic vesicle proteins within single retinotectal axons of Xenopus tadpoles.
1998,
Pubmed
,
Xenbase
Poo,
Neurotrophins as synaptic modulators.
2001,
Pubmed
Pozzo-Miller,
Impairments in high-frequency transmission, synaptic vesicle docking, and synaptic protein distribution in the hippocampus of BDNF knockout mice.
1999,
Pubmed
Reh,
Retinal ganglion cell terminals change their projection sites during larval development of Rana pipiens.
1984,
Pubmed
Rodríguez,
Long-term potentiation in the rat dentate gyrus is associated with enhanced Arc/Arg3.1 protein expression in spines, dendrites and glia.
2005,
Pubmed
Ruthazer,
Stabilization of axon branch dynamics by synaptic maturation.
2006,
Pubmed
,
Xenbase
Rybicka,
Ultrastructure and GABA immunoreactivity in layers 8 and 9 of the optic tectum of Xenopus laevis.
1994,
Pubmed
,
Xenbase
Sanchez,
BDNF increases synapse density in dendrites of developing tectal neurons in vivo.
2006,
Pubmed
,
Xenbase
Schikorski,
Quantitative ultrastructural analysis of hippocampal excitatory synapses.
1997,
Pubmed
Schikorski,
Morphological correlates of functionally defined synaptic vesicle populations.
2001,
Pubmed
Skoff,
Fine structure of dendritic and axonal growth cones in embryonic chick spinal cord.
1974,
Pubmed
Soppet,
The neurotrophic factors brain-derived neurotrophic factor and neurotrophin-3 are ligands for the trkB tyrosine kinase receptor.
1991,
Pubmed
Sorra,
Critical assessment of the involvement of perforations, spinules, and spine branching in hippocampal synapse formation.
1998,
Pubmed
Squinto,
trkB encodes a functional receptor for brain-derived neurotrophic factor and neurotrophin-3 but not nerve growth factor.
1991,
Pubmed
Swanwick,
Synaptic and extrasynaptic localization of brain-derived neurotrophic factor and the tyrosine kinase B receptor in cultured hippocampal neurons.
2004,
Pubmed
Tao,
Retrograde signaling at central synapses.
2001,
Pubmed
Tao,
Emergence of input specificity of ltp during development of retinotectal connections in vivo.
2001,
Pubmed
,
Xenbase
Tao,
Activity-dependent matching of excitatory and inhibitory inputs during refinement of visual receptive fields.
2005,
Pubmed
,
Xenbase
Tashiro,
Bidirectional regulation of hippocampal mossy fiber filopodial motility by kainate receptors: a two-step model of synaptogenesis.
2003,
Pubmed
Tyler,
BDNF enhances quantal neurotransmitter release and increases the number of docked vesicles at the active zones of hippocampal excitatory synapses.
2001,
Pubmed
Tyler,
BDNF increases release probability and the size of a rapidly recycling vesicle pool within rat hippocampal excitatory synapses.
2006,
Pubmed
Tyler,
Miniature synaptic transmission and BDNF modulate dendritic spine growth and form in rat CA1 neurones.
2003,
Pubmed
Vaughn,
Fine structure of synaptogenesis in the vertebrate central nervous system.
1989,
Pubmed
Wang,
Presynaptic neurotrophin-3 increases the number of tectal synapses, vesicle density, and number of docked vesicles in chick embryos.
2003,
Pubmed
Wittig,
Synaptic ribbon enables temporal precision of hair cell afferent synapse by increasing the number of readily releasable vesicles: a modeling study.
2008,
Pubmed
Wong,
Rapid dendritic movements during synapse formation and rearrangement.
2000,
Pubmed
Yen,
Fine-structural alterations and clustering of developing synapses after chronic treatments with low levels of NMDA.
1993,
Pubmed
Yuste,
Genesis of dendritic spines: insights from ultrastructural and imaging studies.
2004,
Pubmed
Yuste,
Morphological changes in dendritic spines associated with long-term synaptic plasticity.
2001,
Pubmed
Zhang,
A critical window for cooperation and competition among developing retinotectal synapses.
1998,
Pubmed
,
Xenbase
Ziv,
Evidence for a role of dendritic filopodia in synaptogenesis and spine formation.
1996,
Pubmed
