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Understanding mechanisms of post-transcriptional control of gene expression has come under much scrutiny in recent years. A key question in this field is how the translation of specific mRNAs is activated or repressed both spatially and temporally in a given cell. In oocytes of the frog Xenopus laevis a number of mRNAs are localized early in oogenesis and subsequently translated at later stages. We have developed a highly active cell-free translation system from oocytes in the early stages of oogenesis that is applicable to the study of translation and translational control of both endogenous and exogenous mRNAs.
Acker,
Reconstitution of yeast translation initiation.
2007, Pubmed
Acker,
Reconstitution of yeast translation initiation.
2007,
Pubmed
Beckler,
In vitro translation using rabbit reticulocyte lysate.
1995,
Pubmed
Besse,
Translational control of localized mRNAs: restricting protein synthesis in space and time.
2008,
Pubmed
Birsoy,
Vg 1 is an essential signaling molecule in Xenopus development.
2006,
Pubmed
,
Xenbase
Castagnetti,
Control of oskar mRNA translation by Bruno in a novel cell-free system from Drosophila ovaries.
2000,
Pubmed
Colegrove-Otero,
The Xenopus ELAV protein ElrB represses Vg1 mRNA translation during oogenesis.
2005,
Pubmed
,
Xenbase
de Moor,
Mechanisms of translational control by the 3' UTR in development and differentiation.
2005,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Dworkin,
Mobilization of specific maternal RNA species into polysomes after fertilization in Xenopus laevis.
1985,
Pubmed
,
Xenbase
Fan-Minogue,
Distinct eRF3 requirements suggest alternate eRF1 conformations mediate peptide release during eukaryotic translation termination.
2008,
Pubmed
Kain,
Overview of genetic reporter systems.
2001,
Pubmed
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Matthews,
A highly efficient, cell-free translation/translocation system prepared from Xenopus eggs.
1991,
Pubmed
,
Xenbase
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Melton,
Translocation of a localized maternal mRNA to the vegetal pole of Xenopus oocytes.
,
Pubmed
,
Xenbase
Murray,
Cell cycle extracts.
1991,
Pubmed
Otero,
A 250-nucleotide UA-rich element in the 3' untranslated region of Xenopus laevis Vg1 mRNA represses translation both in vivo and in vitro.
2001,
Pubmed
,
Xenbase
Patrick,
Preparation and characterization of cell-free protein synthesis systems from oocytes and eggs of Xenopus laevis.
1989,
Pubmed
,
Xenbase
Pickering,
The implications of structured 5' untranslated regions on translation and disease.
2005,
Pubmed
Pisarev,
Assembly and analysis of eukaryotic translation initiation complexes.
2007,
Pubmed
Pisarev,
Recycling of eukaryotic posttermination ribosomal complexes.
2007,
Pubmed
Stebbins-Boaz,
Maskin is a CPEB-associated factor that transiently interacts with elF-4E.
1999,
Pubmed
,
Xenbase
Thermann,
Drosophila miR2 induces pseudo-polysomes and inhibits translation initiation.
2007,
Pubmed
Van Herwynen,
Translation using a wheat-germ extract.
1995,
Pubmed
Woodland,
Utilization of stored mRNA in Xenopus embryos and its replacement by newly synthesized transcripts: histone H1 synthesis using interspecies hybrids.
1979,
Pubmed
,
Xenbase
Yisraeli,
Synthesis of long, capped transcripts in vitro by SP6 and T7 RNA polymerases.
1989,
Pubmed
Zhou,
Localization of Xcat-2 RNA, a putative germ plasm component, to the mitochondrial cloud in Xenopus stage I oocytes.
1996,
Pubmed
,
Xenbase
