Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Neurosci Lett
2010 Sep 06;4812:115-9. doi: 10.1016/j.neulet.2010.06.066.
Show Gene links
Show Anatomy links
Neurite outgrowth from PC12 cells is enhanced by an inhibitor of mechanical channels.
Gottlieb PA
,
Barone T
,
Sachs F
,
Plunkett R
.
???displayArticle.abstract???
GsMTx4, a peptide inhibitor for mechanosensitive ion channels (MSCs), promoted neurite outgrowth from PC12 cells in the presence of NGF in a dose-dependent manner between 5 and 100 microM peptide. Enhanced neurite growth required >12 h of peptide exposure in cells grown with NGF. Adsorption of GsMTx4 to serum proteins in the media lowered the free peptide concentration of 100 microM to a free concentration of 5 microM, a concentration shown to completely inhibit MSCs in the patch clamp assay. Outside-out patches from PC12 cells grown in NGF had mechanically activated cation channels that were reversibly inhibited by GsMTx4. These results are similar to those observed by Gomez and co-workers in Xenopus spinal cord. The inhibition of mechanosensitive channels by GsMTx4 may be a useful approach to accelerate regeneration of neurons in neurodegenerative diseases and spinal cord injury.
???displayArticle.pubmedLink???
20600595
???displayArticle.pmcLink???PMC2922024 ???displayArticle.link???Neurosci Lett ???displayArticle.grants???[+]
Bowman,
Mechanosensitive ion channels and the peptide inhibitor GsMTx-4: history, properties, mechanisms and pharmacology.
2007, Pubmed
Bowman,
Mechanosensitive ion channels and the peptide inhibitor GsMTx-4: history, properties, mechanisms and pharmacology.
2007,
Pubmed
Chen,
Angiopoietin-1 induces neurite outgrowth of PC12 cells in a Tie2-independent, beta1-integrin-dependent manner.
2009,
Pubmed
Gottlieb,
Mechanosensitive ion channels as drug targets.
2004,
Pubmed
Jacques-Fricke,
Ca2+ influx through mechanosensitive channels inhibits neurite outgrowth in opposition to other influx pathways and release from intracellular stores.
2006,
Pubmed
,
Xenbase
Lu,
Axon regeneration through scars and into sites of chronic spinal cord injury.
2007,
Pubmed
Ostrow,
cDNA sequence and in vitro folding of GsMTx4, a specific peptide inhibitor of mechanosensitive channels.
2003,
Pubmed
Oswald,
Solution structure of peptide toxins that block mechanosensitive ion channels.
2002,
Pubmed
Peterson,
Treatment of Parkinson's disease with trophic factors.
2008,
Pubmed
Posokhov,
Is lipid bilayer binding a common property of inhibitor cysteine knot ion-channel blockers?
2007,
Pubmed
Posokhov,
Quenching-enhanced fluorescence titration protocol for accurate determination of free energy of membrane binding.
2007,
Pubmed
Suchyna,
Identification of a peptide toxin from Grammostola spatulata spider venom that blocks cation-selective stretch-activated channels.
2000,
Pubmed
Suchyna,
Bilayer-dependent inhibition of mechanosensitive channels by neuroactive peptide enantiomers.
2004,
Pubmed
Tuszynski,
Nerve growth factor gene therapy in Alzheimer disease.
2007,
Pubmed
Wei,
Calcium flickers steer cell migration.
2009,
Pubmed
Yang,
Effect of scoparone on neurite outgrowth in PC12 cells.
2008,
Pubmed
