Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Am J Physiol Cell Physiol
2010 Aug 01;2992:C251-63. doi: 10.1152/ajpcell.00091.2009.
Show Gene links
Show Anatomy links
Cloning and identification of tissue-specific expression of KCNN4 splice variants in rat colon.
Barmeyer C
,
Rahner C
,
Yang Y
,
Sigworth FJ
,
Binder HJ
,
Rajendran VM
.
???displayArticle.abstract???
KCNN4 channels that provide the driving force for cAMP- and Ca(2+)-induced anion secretion are present in both apical and basolateral membranes of the mammalian colon. However, only a single KCNN4 has been cloned. This study was initiated to identify whether both apical and basolateral KCNN4 channels are encoded by the same or different isoforms. Reverse transcriptase-PCR (RT-PCR), real-time quantitative-PCR (RT-QPCR), and immunofluorescence studies were used to clone and identify tissue-specific expression of KCNN4 isoforms. Three distinct KCNN4 cDNAs that are designated as KCNN4a, KCNN4b, and KCNN4c encoding 425, 424, and 395 amino acid proteins, respectively, were isolated from the rat colon. KCNN4a differs from KCNN4b at both the nucleotide and the amino acid level with distinct 628 bp at the 3'-untranslated region and an additional glutamine at position 415, respectively. KCNN4c differs from KCNN4b by lacking the second exon that encodes a 29 amino acid motif. KCNN4a and KCNN4b/c are identified as smooth muscle- and epithelial cell-specific transcripts, respectively. KCNN4b and KCNN4c transcripts likely encode basolateral (40 kDa) and apical (37 kDa) membrane proteins in the distal colon, respectively. KCNN4c, which lacks the S2 transmembrane segment, requires coexpression of a large conductance K(+) channel beta-subunit for plasma membrane expression. The KCNN4 channel blocker TRAM-34 inhibits KCNN4b- and KCNN4c-mediated (86)Rb (K(+) surrogate) efflux with an apparent inhibitory constant of 0.6 +/- 0.1 and 7.8 +/- 0.4 muM, respectively. We conclude that apical and basolateral KCNN4 K(+) channels that regulate K(+) and anion secretion are encoded by distinct isoforms in colonic epithelial cells.
Albaqumi,
KCa3.1 potassium channels are critical for cAMP-dependent chloride secretion and cyst growth in autosomal-dominant polycystic kidney disease.
2008, Pubmed
Albaqumi,
KCa3.1 potassium channels are critical for cAMP-dependent chloride secretion and cyst growth in autosomal-dominant polycystic kidney disease.
2008,
Pubmed
Bakos,
Functional multidrug resistance protein (MRP1) lacking the N-terminal transmembrane domain.
1998,
Pubmed
Barrett,
Chloride secretion by the intestinal epithelium: molecular basis and regulatory aspects.
2000,
Pubmed
Begenisich,
Physiological roles of the intermediate conductance, Ca2+-activated potassium channel Kcnn4.
2004,
Pubmed
Bernard,
Modulation of calcium-dependent chloride secretion by basolateral SK4-like channels in a human bronchial cell line.
2003,
Pubmed
Biber,
Isolation of basolateral membranes from columnar cells of the proximal colon of the guinea pig.
1983,
Pubmed
Bleich,
Ca2+ regulated K+ and non-selective cation channels in the basolateral membrane of rat colonic crypt base cells.
1996,
Pubmed
Breitwieser,
Mechanisms of K+ channel regulation.
1996,
Pubmed
Chen,
Small and intermediate conductance Ca(2+)-activated K+ channels confer distinctive patterns of distribution in human tissues and differential cellular localisation in the colon and corpus cavernosum.
2004,
Pubmed
Devor,
Modulation of Cl- secretion by benzimidazolones. II. Coordinate regulation of apical GCl and basolateral GK.
1996,
Pubmed
Fanger,
Calcium-activated potassium channels sustain calcium signaling in T lymphocytes. Selective blockers and manipulated channel expression levels.
2001,
Pubmed
Fong,
Characterization of vectorial chloride transport pathways in the human pancreatic duct adenocarcinoma cell line HPAF.
2003,
Pubmed
Fordtran,
Speculations on the pathogenesis of diarrhea.
1967,
Pubmed
Furness,
Expression of intermediate conductance potassium channel immunoreactivity in neurons and epithelial cells of the rat gastrointestinal tract.
2003,
Pubmed
Gottardi,
Sorting of ion transport proteins in polarized cells.
1993,
Pubmed
Greger,
New types of K+ channels in the colon.
1997,
Pubmed
Halm,
Distinct K+ conductive pathways are required for Cl- and K+ secretion across distal colonic epithelium.
2006,
Pubmed
Hanley,
Extracellular ATP induces oscillations of intracellular Ca2+ and membrane potential and promotes transcription of IL-6 in macrophages.
2004,
Pubmed
Ishii,
A human intermediate conductance calcium-activated potassium channel.
1997,
Pubmed
,
Xenbase
Jensen,
Characterization of the cloned human intermediate-conductance Ca2+-activated K+ channel.
1998,
Pubmed
Joiner,
Active K+ secretion through multiple KCa-type channels and regulation by IKCa channels in rat proximal colon.
2003,
Pubmed
Joiner,
hSK4, a member of a novel subfamily of calcium-activated potassium channels.
1997,
Pubmed
Jung,
Topology and function of the Na+/proline transporter of Escherichia coli, a member of the Na+/solute cotransporter family.
1998,
Pubmed
Kast,
Membrane topology of P-glycoprotein as determined by epitope insertion: transmembrane organization of the N-terminal domain of mdr3.
1995,
Pubmed
Kast,
Topology mapping of the amino-terminal half of multidrug resistance-associated protein by epitope insertion and immunofluorescence.
1997,
Pubmed
Kolski-Andreaco,
SK3-1C, a dominant-negative suppressor of SKCa and IKCa channels.
2004,
Pubmed
Krogh,
Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
2001,
Pubmed
Lauf,
Apparent intermediate K conductance channel hyposmotic activation in human lens epithelial cells.
2008,
Pubmed
Liou,
Improving structural integrity of cryosections for immunogold labeling.
1996,
Pubmed
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Logsdon,
A novel gene, hKCa4, encodes the calcium-activated potassium channel in human T lymphocytes.
1997,
Pubmed
Lomax,
Aldosterone-induced apical Na+ and K+ conductances are located predominantly in surface cells in rat distal colon.
1994,
Pubmed
Meera,
Large conductance voltage- and calcium-dependent K+ channel, a distinct member of voltage-dependent ion channels with seven N-terminal transmembrane segments (S0-S6), an extracellular N terminus, and an intracellular (S9-S10) C terminus.
1997,
Pubmed
Mense,
Extracellular domains, transmembrane segments, and intracellular domains interact to determine the cation selectivity of Na,K- and gastric H,K-ATPase.
2002,
Pubmed
Muth,
Transport protein trafficking in polarized cells.
2003,
Pubmed
Neylon,
Molecular cloning and characterization of the intermediate-conductance Ca(2+)-activated K(+) channel in vascular smooth muscle: relationship between K(Ca) channel diversity and smooth muscle cell function.
1999,
Pubmed
,
Xenbase
Nimigean,
Functional coupling of the beta(1) subunit to the large conductance Ca(2+)-activated K(+) channel in the absence of Ca(2+). Increased Ca(2+) sensitivity from a Ca(2+)-independent mechanism.
2000,
Pubmed
Rajendran,
Sodium uptake across basolateral membrane of rat distal colon. Evidence for Na-H exchange and Na-anion cotransport.
1991,
Pubmed
Rajendran,
Aldosterone induction of electrogenic sodium transport in the apical membrane vesicles of rat distal colon.
1989,
Pubmed
Reyes,
Cloning and expression of a novel pH-sensitive two pore domain K+ channel from human kidney.
1998,
Pubmed
Rufo,
The antifungal antibiotic, clotrimazole, inhibits Cl- secretion by polarized monolayers of human colonic epithelial cells.
1996,
Pubmed
Sandle,
Potassium channels in colonic crypts.
1994,
Pubmed
Sangan,
Colonic H-K-ATPase alpha- and beta-subunits express ouabain-insensitive H-K-ATPase.
2000,
Pubmed
,
Xenbase
Sangan,
Colonic H-K-ATPase beta-subunit: identification in apical membranes and regulation by dietary K depletion.
1999,
Pubmed
Sharma,
Mechanism of substance P-induced hyperpolarization of porcine coronary artery endothelial cells.
1994,
Pubmed
Shepherd,
KCa3.1 Ca2+ activated K+ channels regulate human airway smooth muscle proliferation.
2007,
Pubmed
Singh,
Benzimidazolone activators of chloride secretion: potential therapeutics for cystic fibrosis and chronic obstructive pulmonary disease.
2001,
Pubmed
,
Xenbase
Stevenson,
Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia.
1986,
Pubmed
Stieger,
Isolation of brush-border membranes from rat and rabbit colonocytes: is alkaline phosphatase a marker enzyme?
1986,
Pubmed
Tao,
Regulation of cell proliferation by intermediate-conductance Ca2+-activated potassium and volume-sensitive chloride channels in mouse mesenchymal stem cells.
2008,
Pubmed
Toyama,
The intermediate-conductance calcium-activated potassium channel KCa3.1 contributes to atherogenesis in mice and humans.
2008,
Pubmed
Vandorpe,
cDNA cloning and functional characterization of the mouse Ca2+-gated K+ channel, mIK1. Roles in regulatory volume decrease and erythroid differentiation.
1998,
Pubmed
,
Xenbase
Wang,
IK channels are involved in the regulatory volume decrease in human epithelial cells.
2003,
Pubmed
Warth,
Molecular and functional characterization of the small Ca(2+)-regulated K+ channel (rSK4) of colonic crypts.
1999,
Pubmed
,
Xenbase
Wulff,
Design of a potent and selective inhibitor of the intermediate-conductance Ca2+-activated K+ channel, IKCa1: a potential immunosuppressant.
2000,
Pubmed
