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XB-ART-42447
Cold Spring Harb Protoc 2010 Dec 01;201012:pdb.prot5538. doi: 10.1101/pdb.prot5538.
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Microinjection of RNA and preparation of secreted proteins from Xenopus oocytes.



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Microinjection of Xenopus oocytes or embryos with messenger RNA (mRNA) is a powerful technique for studying development, including protein expression during development. This protocol describes a method for the preparation of secreted (35)S-labeled proteins following mRNA microinjection into Xenopus oocytes.

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