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XB-ART-42448
Cold Spring Harb Protoc 2010 Dec 01;201012:pdb.prot5537. doi: 10.1101/pdb.prot5537.
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Calibration of the injection volume for microinjection of Xenopus oocytes and embryos.



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Microinjection of Xenopus oocytes or embryos with messenger RNA (mRNA) or DNA is a powerful technique for studying development. Before microinjection can be performed, the injection volume must be calibrated carefully. This protocol describes the calibration procedure for a pressure injector.

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