Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biochim Biophys Acta
2011 Jun 01;18136:1129-36. doi: 10.1016/j.bbamcr.2011.01.002.
Show Gene links
Show Anatomy links
Biphasic chromatin binding of histone chaperone FACT during eukaryotic chromatin DNA replication.
Kundu LR
,
Seki M
,
Watanabe N
,
Murofushi H
,
Furukohri A
,
Waga S
,
Score AJ
,
Blow JJ
,
Horikoshi M
,
Enomoto T
,
Tada S
.
???displayArticle.abstract???
The facilitates chromatin transcription (FACT) complex affects nuclear DNA transactions in a chromatin context. Though the involvement of FACT in eukaryotic DNA replication has been revealed, a clear understanding of its biochemical behavior during DNA replication still remains elusive. Here, we analyzed the chromatin-binding dynamics of FACT using Xenopus egg extract cell-free system. We found that FACT has at least two distinct chromatin-binding phases: (1) a rapid chromatin-binding phase at the onset of DNA replication that did not involve origin licensing and (2) a second phase of chromatin binding that initiated after origin licensing. Intriguingly, early-binding FACT dissociated from chromatin when DNA replication was blocked by the addition of Cdc6 in the licensed state before origin firing. Cdc6-induced removal of FACT was blocked by the inhibition of origin licensing with geminin, but not by suppressing the activity of DNA polymerases, CDK, or Cdc7. Furthermore, chromatin transfer experiments revealed that impairing the later binding of FACT severely compromises DNA replication activity. Taken together, we propose that even though FACT has rapid chromatin-binding activity, the binding pattern of FACT on chromatin changes after origin licensing, which may contribute to the establishment of its functional link to the DNA replication machinery.
Alexiadis,
Influence of core histone acetylation on SV40 minichromosome replication in vitro.
1997, Pubmed
Alexiadis,
Influence of core histone acetylation on SV40 minichromosome replication in vitro.
1997,
Pubmed
Alexiadis,
In vitro chromatin remodelling by chromatin accessibility complex (CHRAC) at the SV40 origin of DNA replication.
1998,
Pubmed
Bell,
DNA replication in eukaryotic cells.
2002,
Pubmed
Belotserkovskaya,
FACT facilitates transcription-dependent nucleosome alteration.
2003,
Pubmed
Brewster,
A bipartite yeast SSRP1 analog comprised of Pob3 and Nhp6 proteins modulates transcription.
2001,
Pubmed
Budd,
A network of multi-tasking proteins at the DNA replication fork preserves genome stability.
2005,
Pubmed
Byun,
Functional uncoupling of MCM helicase and DNA polymerase activities activates the ATR-dependent checkpoint.
2005,
Pubmed
,
Xenbase
Chong,
Characterization of the Xenopus replication licensing system.
1997,
Pubmed
,
Xenbase
Coleman,
The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts.
1996,
Pubmed
,
Xenbase
Formosa,
Spt16-Pob3 and the HMG protein Nhp6 combine to form the nucleosome-binding factor SPN.
2001,
Pubmed
Formosa,
Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure.
2002,
Pubmed
Furukohri,
Identification and characterization of a Xenopus homolog of Dbf4, a regulatory subunit of the Cdc7 protein kinase required for the initiation of DNA replication.
2003,
Pubmed
,
Xenbase
Gambus,
GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks.
2006,
Pubmed
Gavin,
Conserved initiator proteins in eukaryotes.
1995,
Pubmed
Grallert,
The ORC1 homolog orp1 in fission yeast plays a key role in regulating onset of S phase.
1996,
Pubmed
Han,
Ubiquitylation of FACT by the cullin-E3 ligase Rtt101 connects FACT to DNA replication.
2010,
Pubmed
Hua,
Identification of a preinitiation step in DNA replication that is independent of origin recognition complex and cdc6, but dependent on cdk2.
1998,
Pubmed
,
Xenbase
Iizuka,
Histone acetyltransferase HBO1 interacts with the ORC1 subunit of the human initiator protein.
1999,
Pubmed
Ishimi,
Binding of human minichromosome maintenance proteins with histone H3.
1996,
Pubmed
Keller,
p53 serine 392 phosphorylation increases after UV through induction of the assembly of the CK2.hSPT16.SSRP1 complex.
2002,
Pubmed
Khoudoli,
Temporal profiling of the chromatin proteome reveals system-wide responses to replication inhibition.
2008,
Pubmed
,
Xenbase
Kubota,
A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication.
2003,
Pubmed
,
Xenbase
Kundu,
Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex.
2010,
Pubmed
,
Xenbase
Lipford,
Nucleosomes positioned by ORC facilitate the initiation of DNA replication.
2001,
Pubmed
Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase
O'Donnell,
Domain organization of the yeast histone chaperone FACT: the conserved N-terminal domain of FACT subunit Spt16 mediates recovery from replication stress.
2004,
Pubmed
Oehlmann,
The role of Cdc6 in ensuring complete genome licensing and S phase checkpoint activation.
2004,
Pubmed
,
Xenbase
Okuhara,
A DNA unwinding factor involved in DNA replication in cell-free extracts of Xenopus eggs.
1999,
Pubmed
,
Xenbase
Orphanides,
The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins.
1999,
Pubmed
Rowles,
Interaction between the origin recognition complex and the replication licensing system in Xenopus.
1996,
Pubmed
,
Xenbase
Rowles,
Changes in association of the Xenopus origin recognition complex with chromatin on licensing of replication origins.
1999,
Pubmed
,
Xenbase
Saunders,
Tracking FACT and the RNA polymerase II elongation complex through chromatin in vivo.
2003,
Pubmed
Schlesinger,
POB3 is required for both transcription and replication in the yeast Saccharomyces cerevisiae.
2000,
Pubmed
Sheu,
Cdc7-Dbf4 phosphorylates MCM proteins via a docking site-mediated mechanism to promote S phase progression.
2006,
Pubmed
Shimojima,
Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor.
2003,
Pubmed
Strausfeld,
Cip1 blocks the initiation of DNA replication in Xenopus extracts by inhibition of cyclin-dependent kinases.
1994,
Pubmed
,
Xenbase
Tada,
Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin.
2001,
Pubmed
,
Xenbase
Takahashi,
Cdc7-Drf1 is a developmentally regulated protein kinase required for the initiation of vertebrate DNA replication.
2005,
Pubmed
,
Xenbase
Tan,
Functional cooperation between FACT and MCM helicase facilitates initiation of chromatin DNA replication.
2006,
Pubmed
Tan,
Functional cooperation between FACT and MCM is coordinated with cell cycle and differential complex formation.
2010,
Pubmed
Tsuyama,
Licensing for DNA replication requires a strict sequential assembly of Cdc6 and Cdt1 onto chromatin in Xenopus egg extracts.
2005,
Pubmed
,
Xenbase
VanDemark,
The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition.
2006,
Pubmed
Varga-Weisz,
Chromatin remodeling factors and DNA replication.
2005,
Pubmed
Wittmeyer,
The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like protein.
1997,
Pubmed
Wittmeyer,
Spt16 and Pob3 of Saccharomyces cerevisiae form an essential, abundant heterodimer that is nuclear, chromatin-associated, and copurifies with DNA polymerase alpha.
1999,
Pubmed
Wohlschlegel,
Inhibition of eukaryotic DNA replication by geminin binding to Cdt1.
2000,
Pubmed
,
Xenbase
Zhou,
A coordinated temporal interplay of nucleosome reorganization factor, sister chromatin cohesion factor, and DNA polymerase alpha facilitates DNA replication.
2004,
Pubmed
