Peyrot SM , Wallingford JB , Harland RM .

GM 42341 NIGMS NIH HHS , R01 GM042341-26 NIGMS NIH HHS , R01-GM074104-01 NIGMS NIH HHS , R01 GM042341 NIGMS NIH HHS , R01 GM074104 NIGMS NIH HHS

Xla Wt + {ca}notch1 (fig.1.f)
Xla Wt + {ca}notch1 (fig.1.i)
Xla Wt + {ca}notch1 (fig.1.i, l)
Xla Wt + {ca}notch1 (fig.1.l)
Xla Wt + {ca}notch1 (fig.1.o)
Xla Wt + {ca}notch1 (fig.1.o)
Xla Wt + {ca}notch1 (fig.2.c, c^1)
Xla Wt + {ca}notch1 (fig.2.d, d^1)
Xla Wt + {ca}notch1 (fig.2.f)
Xla Wt + {ca}notch1 (fig.2.g, h)
Xla Wt + {ca}notch1 (fig.2.k, l)
Xla Wt + {ca}notch1 (fig.4.i)
Xla Wt + {ca}notch1 (fig.4.i)
Xla Wt + {ca}notch1 (fig.6.b, f)
Xla Wt + {ca}notch1 (fig.7.i, i^1)
Xla Wt + {ca}notch1 (fig.S1.b, c, e, f)
Xla Wt + {ca}notch1 (fig.S1.b, e)
Xla Wt + {ca}notch1 (fig.S1.c, f)
Xla Wt + Cyclopamine (fig.3.b)
Xla Wt + Cyclopamine (fig.3.e)
Xla Wt + Cyclopamine (fig.5.h)
Xla Wt + Cyclopamine (fig.6.g)
Xla Wt + Cyclopamine (fig.S2.h)
Xla Wt + Cyclopamine (fig.S3. b)
Xla Wt + Cyclopamine (fig.S3. b^1)
Xla Wt + Cyclopamine (fig.S3. b^1^1)
Xla Wt + Cyclopamine (fig.S4.b)
Xla Wt + Cyclopamine (fig.S4.e)
Xla Wt + {dn}rbpj (fig.1.b)
Xla Wt + {dn}rbpj (fig.1.h)
Xla Wt + {dn}rbpj (fig.1.k)
Xla Wt + {dn}rbpj (fig.1.n)
Xla Wt + {dn}rbpj (fig.1.n)
Xla Wt + {dn}rbpj (fig.2.b, b^1)
Xla Wt + {dn}rbpj (fig.6.j)
Xla Wt + {dn}rbpj (fig.7.h, h^1)
Xla Wt + shh (fig.3.c)
Xla Wt + shh (fig.5.i)
Xla Wt + shh (fig.6.i)
Xla Wt + shh (fig.S2.d)
Xla Wt + shh (fig.S3.e)
Xla Wt + shh (fig.S3.e')
Xla Wt + shh (fig.S3.e^1)
Xla Wt + shh MO (fig.3.b)
Xla Wt + shh MO (fig.3.e)
Xla Wt + shh MO (fig.5.h)
Xla Wt + shh MO (fig.6.c)
Xla Wt + shh MO (fig.S2.e, i)
Xla Wt + shh MO (fig.S3.c)
Xla Wt + shh MO (fig.S3.c')
Xla Wt + shh MO (fig.S3.c^1)
Xla Wt + shh MO (fig.S3.d)
Xla Wt + shh MO (fig.S3.d')
Xla Wt + shh MO (fig.S3.d^1)

	Fig. 2. Perturbation of Notch signaling has variable effects on Shh expression. Shh expression in neurula (st.16, A–H) and tadpole (st.35, I–L) embryos upon blockade (B, F, J) and activation (C, D, E–H, K, L) of Notch signaling. Shh is expressed in the floor plate and notochord (A, A', I, I'). Blockade of Notch signaling led to a slight reduction in the floor plate domain of Shh in 31% of embryos (B, B'), but many embryos showed normal expression (J', 69%). Activated Notch signaling led to increased expression throughout the midline (C, C'), though the floor plate domain was normal (F, K'), in 27% of embryos. In a significant proportion of embryos (69%, p < 0.05), activated Notch led to a decrease in Shh expression throughout the midline (H, L') or in the floor plate domain (G). In NICD tadpoles, Shh expression was decreased and/or patchy in the spinal cord (K, L). The variable response of Shh to Notch activation is not correlated with amount or distribution of NICD protein (E–H, αnti-myc staining for NICD-myc in brown). NICD in the lateral neural plate does not cause ectopic Shh expression (E), while NICD in the FP domain can result in Shh expression (F), or repression (G, H). (A'–D') Transverse sections of (A–D); faint notochord expression of Shh is not visible in thin sections. (E–H) αnti-myc staining (brown) was performed on transversely bisected embryos after ISH for Shh (purple). The notochord is outlined in red and the ventral boundary of the neural plate in blue. (I–L) Lateral view of tailbud stage embryos. (I'–L') Transverse vibratome sections through middle of spinal cord at tailbud stages.
	Fig.3. Shh is not a major regulator of dorsal midline fates. Shh signaling was blocked by injection of Shh MO or by cyclopamine treatment (“−Shh” B, E, G, J, M, P) and activated by injection of Shh mRNA (“+Shh,” C, H, K, N, Q). Netrin expression in the floor plate was decreased in 42% of − Shh embryos (B, N = 387) and slightly upregulated in 44% of + Shh embryos (C, N = 139). Floor plate expression of Shh was slightly narrower in 29% of embryos with blockade of Shh signaling (E inset, N = 194). Perturbation of Shh signaling had no effect on notochord (AxPC, F–H and Tor70, I–Q) or hypochord (O–Q) or floor plate markers F-spondin and FoxA2 in the spinal cord of tadpoles (I–N). (A–H) Dorsal views, anterior up, with transverse bisection in inset (A–E). (G–O) Transverse vibratome sections through middle of spinal cord at tailbud stages.
	Fig.4. Ectopic Shh does not affect notochord formation and Notch signaling acts after specification to repress the notochord fate. Shh mRNA overexpression does not repress notochord specification at stage 10, assayed by chd (B) and Xbra (E), or notochord formation during gastrulation (Xbra, H). Activated Notch signaling does not affect notochord specification (C, F), but potently represses subsequent notochord formation (I). Pink is lacZ staining of co-injected lineage tracer.
	Fig.5. Lateral floor plate is regulated by Shh signaling. Nkx2.2 is a marker of the LFP in zebrafish and chick. At onset in frog (stage 22), it is expressed in two stripes in the ventral neural tube (C) lateral to Shh (A) and Netrin (B). At tadpole stages, Shh (D), Netrin (E), and Nkx2.2 (F) clearly mark distinct domains. LFP formation requires Shh signaling (H) and Shh overexpression can induce ectopic Nkx2.2 throughout the brain and upregulation in the spinal cord (I) at stage 22. (A–F) Transverse vibratome sections following ISH and Tor70 staining of the notochord (brown, D–F). (G–I) Dorsal views, anterior up.
	Fig.6. Shh and Notch signaling act in parallel to specify floor plate. Epistasis (A–L) and enhancement (M–P) experiments to determine the contribution of Notch and Shh signaling to floor plate formation. Embryos were injected and/or treated with reagents listed, fixed at stage 20–22, bisected and stained for co-injected lineage tracer (pink), then processed for Netrin ISH. Activated Notch signaling upregulates Netrin (B, F), while blockade of Shh signaling slightly reduces Netrin (C, G). Activation of Notch and blockade of Shh in the same embryo results in a Notch phenotype (D, H). Shh overexpression leads to a slight increase in FP (I), and blockade of Notch signaling leads to a decrease (J). Activation of Shh and blockade of Notch in the same embryo results in a Notch phenotype (K). Blockade of Notch signaling downstream of NICD with SDBM is able to rescue FP and notochord development to normal (L). Blockade (M, O) or activation (P) of both Notch and Shh signaling in the same embryo gives more severe phenotypes than perturbing either pathway alone.
	Fig.7. Notch signaling does not regulate gastrula midline markers or 1° MFP. Shh and plvs are markers of 1° MFP ( Fig. S6). Blockade (B, E, H) and activation (C, F, I) of Notch signaling does not affect gastrula stage expression of these markers (A–F). Expression of plvs upon perturbation of Notch signaling (G–I) looks much like that of Shh (see Fig. 2), with FP expression only mildly affected (G'–I'). (G'–I') Transverse bisections of G–I.
	Fig. 1. Notch signaling promotes formation of floor plate and hypochord and represses notochord. Notch signaling was repressed by injection of mRNA encoding Su(H) DNA-binding mutant (SDBM, B, E, H, K, N) and activated by Notch intracellular domain mRNA (NICD, C, F, I, L, O). Floor plate development was assayed by expression of Netrin (A–C), F-spondin (G–I), and FoxA2 (J–L). Notochord formation was assayed by Axial protocadherin (AxPC, D–F) and Tor70 staining (brown, G–O). Tor70 staining has high variability and background, and thus should not be considered quantitative. Hypochord development was assayed by F-spondin (G–I, staining ventral to notochord) and VEGF (M–O). (A–F) Dorsal views, anterior up. (G–O) Transverse vibratome sections through middle of spinal cord at tailbud stages.
	Fig. S1. Notch-expressing cells do not contribute to notochord. NICD-myc mRNA was injected with (B, C) or without (E, F) β-galactosidase mRNA as a lineage tracer into both dorsal blastomeres to target the midline. Embryos were fixed, bisected transversely, stained for lacZ (pink, A–C), and processed by ISH for AxPC expression in the notochord (purple, A–F). This injection technique efficiently targets the midline (A), with labeled cells in FP, notochord, dorsal endoderm and sometimes medial somite. Embryos with activated Notch have reduced (B, E) or absent (C, F) notochord. Co-injected lineage tracer never labels any notochord that forms (B). Staining for NICD protein (αnti-myc, brown in E, F) shows that NICD+ cells never give rise to the notochord.
	Fig. S2. Shh MO efficiently and specifically blocks Shh function. (A) Schematic of Shh transcript with exons (blue), introns (Vs), and UTRs (gray) shown. “Shh spliceMO” (red) was designed to overlap the first splice donor site. RT-PCR primers (black) were designed flanking the splice junction to assay for correct splicing. (B) RT-PCR of embryos injected with Shh spliceMO shows dose-dependent reduction of Shh splicing. EF1α serves as a loading control. (C–J) ISH for Hh pathway target genes Patched1/2. Shh mRNA upregulates Ptc (D), while Shh MO reduces Ptc (E). Reduction by Shh MO is specific, as it can be rescued by co-injection of Shh mRNA (F). In a separate round of experiments, cyclopamine treatment shows a similar amount of Ptc reduction (H) to Shh MO (I). Combination of these reagents does not enhance the phenotype (J), indicating that maximal possible knockdown of Shh function is achieved by either Shh MO or cyclopamine alone.
	Fig. S3. Loss of Shh signaling and Hh signaling cause similar developmental defects. (A) Control embryos at stage 42, lateral (A), dorsal, (A') and ventral (A") views. (B) Cylopamine treatment blocks all Hh signaling, resulting in reduced melanocytes, edema, and kinked tail (B), closely spaced eyes (B’), and gut-looping defects (B"). Injection of MOs designed to the translation start site (“Shh ATGMO,” C–C") and splice donor site (“Shh spliceMO,” D–D") show similar phenotypes. (E) Injection of Shh mRNA (pink, lacZ staining) results in excess melanocytes (D, arrow), ventralization of the eye (E, E'), and gut looping defects (E").
	Fig. S4. Perturbation of S/Hh signaling only affects the floor plate of the brain. Lateral views of tailbud stage control (A, D), cylopamine-treated (B, E), and Shh mRNA injected (C, F) embryos, processed by ISH for F-spondin (A–C) and FoxA2 (D–F) and stained for notochord (Tor70, brown, A–C). Loss of Hh signaling results in reduced (E) or broken (B) staining of floor plate markers in the brain in 48% of cyclopamine-treated embryos (N = 149). Shh mRNA does not cause dorsal expansion of floor plate except in the brain (arrows, C and F). Pink in C, F is staining for co-injected lineage tracer.
	Fig. S5. Notch and Shh signaling operate in parallel to specify floor plate, but Shh signaling is not required for Notch repression of notochord. Quantification of results shown in Fig. 6. Embryos were injected and/or treated as indicated. At stage 20–22, embryos were fixed, bisected, stained for lacZ lineage tracer, and assayed for FP by Netrin ISH (A, C) and for notochord by Tor70 staining (B). We scored FP development by the intensity of Netrin staining and notochord development by the size of the Tor70+ structure. Total percentages of embryos of each phenotype (see Fig. 6) are represented by stacked column graphs. N is the number of bisected embryos scored in at least 2 independent experiments. For determination of statistical significance, χ2 tests were performed pairwise between 1) controls and single reagents and 2) double treatments and each single component. * significant difference from control, p < 0.05. # significant difference from NICD/SDBM, p < 0.05. + significant difference from other reagent, p < 0.05.
	Fig. S6. Analysis of floor plate markers reveals two separate populations of medial floor plate. In situ hybridization for indicated markers was performed at stage 10.5–11 (A, C, E, G, I, K, M) and neurula stages (st.14—B, D, F, J, L, N, O; st.16—H, P). Prime panels (B', etc.) show transverse bisections of matching neurula stages (notochord outlined in red). Markers expressed in the organizer (marked by asterisk) at gastrula stages are expressed throughout the dorsal midline and in a narrow domain in the floor plate (A–N') at neurula stages. Netrin expression begins at early neurula stages (st.14, O) in a wider domain than early markers (O', P' compared to B'–N'). We have designated the narrow, early-forming FP the 1° MFP and the wider, later-forming FP the 2° FP.
	enc1.2 (ectodermal-neural cortex (with BTB-like domain), gene 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 10.5, vegetal view, dorsal up.
	enc1.2 (ectodermal-neural cortex (with BTB-like domain), gene 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 14, dorsal view, anterior up.

Appel, lunatic fringe regulates Delta-Notch induction of hypochord in zebrafish. 2003, Pubmed

Appel, lunatic fringe regulates Delta-Notch induction of hypochord in zebrafish. 2003, Pubmed
Appel, Delta-mediated specification of midline cell fates in zebrafish embryos. 1999, Pubmed
Appel, Delta-Notch signaling and lateral inhibition in zebrafish spinal cord development. 2001, Pubmed
Artavanis-Tsakonas, Notch signaling: cell fate control and signal integration in development. 1999, Pubmed
Artinger, Delayed formation of the floor plate after ablation of the avian notochord. 1993, Pubmed
Bauer, The cleavage stage origin of Spemann's Organizer: analysis of the movements of blastomere clones before and during gastrulation in Xenopus. 1994, Pubmed , Xenbase
Beddington, Brachyury--a gene affecting mouse gastrulation and early organogenesis. 1992, Pubmed
Bolce, XFKH2, a Xenopus HNF-3 alpha homologue, exhibits both activin-inducible and autonomous phases of expression in early embryos. 1993, Pubmed , Xenbase
Brand, Mutations affecting development of the midline and general body shape during zebrafish embryogenesis. 1996, Pubmed
Briscoe, Specification of neuronal fates in the ventral neural tube. 2001, Pubmed
Briscoe, A homeodomain protein code specifies progenitor cell identity and neuronal fate in the ventral neural tube. 2000, Pubmed
Catala, Organization and development of the tail bud analyzed with the quail-chick chimaera system. 1995, Pubmed
Catala, A spinal cord fate map in the avian embryo: while regressing, Hensen's node lays down the notochord and floor plate thus joining the spinal cord lateral walls. 1996, Pubmed
Charrier, Dual origin of the floor plate in the avian embryo. 2002, Pubmed , Xenbase
Charron, The morphogen sonic hedgehog is an axonal chemoattractant that collaborates with netrin-1 in midline axon guidance. 2003, Pubmed , Xenbase
Chen, Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened. 2002, Pubmed
Chiang, Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function. 1996, Pubmed
Cleaver, Neovascularization of the Xenopus embryo. 1997, Pubmed , Xenbase
Conlon, A primary requirement for nodal in the formation and maintenance of the primitive streak in the mouse. 1994, Pubmed
Dale, Fate map for the 32-cell stage of Xenopus laevis. 1987, Pubmed , Xenbase
de la Torre, Turning of retinal growth cones in a netrin-1 gradient mediated by the netrin receptor DCC. 1997, Pubmed , Xenbase
Dessaud, Dynamic assignment and maintenance of positional identity in the ventral neural tube by the morphogen sonic hedgehog. 2010, Pubmed
Ding, Diminished Sonic hedgehog signaling and lack of floor plate differentiation in Gli2 mutant mice. 1998, Pubmed , Xenbase
Dirksen, A novel, activin-inducible, blastopore lip-specific gene of Xenopus laevis contains a fork head DNA-binding domain. 1992, Pubmed , Xenbase
Donoviel, Mice lacking both presenilin genes exhibit early embryonic patterning defects. 1999, Pubmed
Ekker, Distinct expression and shared activities of members of the hedgehog gene family of Xenopus laevis. 1995, Pubmed , Xenbase
Etheridge, Floor plate develops upon depletion of tiggy-winkle and sonic hedgehog. 2001, Pubmed
Glinka, Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction. 1998, Pubmed , Xenbase
Gray, Notch signalling regulates the contribution of progenitor cells from the chick Hensen's node to the floor plate and notochord. 2010, Pubmed
Gritsman, The EGF-CFC protein one-eyed pinhead is essential for nodal signaling. 1999, Pubmed , Xenbase
Haigo, A family of Xenopus BTB-Kelch repeat proteins related to ENC-1: new markers for early events in floorplate and placode development. 2003, Pubmed , Xenbase
Hatta, The cyclops mutation blocks specification of the floor plate of the zebrafish central nervous system. 1991, Pubmed
Heyer, Postgastrulation Smad2-deficient embryos show defects in embryo turning and anterior morphogenesis. 1999, Pubmed
Jülich, beamter/deltaC and the role of Notch ligands in the zebrafish somite segmentation, hindbrain neurogenesis and hypochord differentiation. 2005, Pubmed
Keller, Vital dye mapping of the gastrula and neurula of Xenopus laevis. II. Prospective areas and morphogenetic movements of the deep layer. 1976, Pubmed , Xenbase
Keller, Vital dye mapping of the gastrula and neurula of Xenopus laevis. I. Prospective areas and morphogenetic movements of the superficial layer. 1975, Pubmed , Xenbase
Kim, The role of paraxial protocadherin in selective adhesion and cell movements of the mesoderm during Xenopus gastrulation. 1998, Pubmed , Xenbase
Klar, F-spondin: a gene expressed at high levels in the floor plate encodes a secreted protein that promotes neural cell adhesion and neurite extension. 1992, Pubmed
Koebernick, A restrictive role for Hedgehog signalling during otic specification in Xenopus. 2003, Pubmed , Xenbase
Kuroda, Axial protocadherin is a mediator of prenotochord cell sorting in Xenopus. 2002, Pubmed , Xenbase
Latimer, Delta-Notch signaling induces hypochord development in zebrafish. 2002, Pubmed
Latimer, Notch signaling regulates midline cell specification and proliferation in zebrafish. 2006, Pubmed
Le Douarin, Discussion point. Origin and specification of the neural tube floor plate: insights from the chick and zebrafish. 2000, Pubmed
Lee, Gli1 is a target of Sonic hedgehog that induces ventral neural tube development. 1997, Pubmed , Xenbase
le Roux, Notch activity is required to maintain floorplate identity and to control neurogenesis in the chick hindbrain and spinal cord. 2003, Pubmed
López, The Notch-target gene hairy2a impedes the involution of notochordal cells by promoting floor plate fates in Xenopus embryos. 2005, Pubmed , Xenbase
López, Notch activates sonic hedgehog and both are involved in the specification of dorsal midline cell-fates in Xenopus. 2003, Pubmed , Xenbase
Martí, Requirement of 19K form of Sonic hedgehog for induction of distinct ventral cell types in CNS explants. 1995, Pubmed
Martin, Hedgehog signaling regulates the amount of hypaxial muscle development during Xenopus myogenesis. 2007, Pubmed , Xenbase
Matise, Gli2 is required for induction of floor plate and adjacent cells, but not most ventral neurons in the mouse central nervous system. 1998, Pubmed
McKanna, Primitive glial compartments in the floor plate of mammalian embryos: distinct progenitors of adult astrocytes and microglia support the notoplate hypothesis. 1993, Pubmed
Minsuk, Surface mesoderm in Xenopus: a revision of the stage 10 fate map. 1997, Pubmed , Xenbase
Müller, Direct action of the nodal-related signal cyclops in induction of sonic hedgehog in the ventral midline of the CNS. 2000, Pubmed
Mumm, Notch signaling: from the outside in. 2000, Pubmed
Odenthal, fork head domain genes in zebrafish. 1998, Pubmed
Odenthal, Two distinct cell populations in the floor plate of the zebrafish are induced by different pathways. 2000, Pubmed
Patten, Distinct modes of floor plate induction in the chick embryo. 2003, Pubmed
Peyrot, Lymph heart musculature is under distinct developmental control from lymphatic endothelium. 2010, Pubmed , Xenbase
Pichon, XHRT-1, a hairy and Enhancer of split related gene with expression in floor plate and hypochord during early Xenopus embryogenesis. 2002, Pubmed , Xenbase
Placzek, Orientation of commissural axons in vitro in response to a floor plate-derived chemoattractant. 1990, Pubmed
Placzek, Discussion point. The case for floor plate induction by the notochord. 2000, Pubmed
Placzek, Mesodermal control of neural cell identity: floor plate induction by the notochord. 1990, Pubmed
Placzek, Induction of floor plate differentiation by contact-dependent, homeogenetic signals. 1993, Pubmed
Przemeck, Node and midline defects are associated with left-right development in Delta1 mutant embryos. 2003, Pubmed
Rebagliati, cyclops encodes a nodal-related factor involved in midline signaling. 1998, Pubmed
Ribes, Distinct Sonic Hedgehog signaling dynamics specify floor plate and ventral neuronal progenitors in the vertebrate neural tube. 2010, Pubmed
Roelink, Floor plate and motor neuron induction by vhh-1, a vertebrate homolog of hedgehog expressed by the notochord. 1994, Pubmed , Xenbase
Roelink, Floor plate and motor neuron induction by different concentrations of the amino-terminal cleavage product of sonic hedgehog autoproteolysis. 1995, Pubmed
Ruiz i Altaba, Restrictions to floor plate induction by hedgehog and winged-helix genes in the neural tube of frog embryos. 1995, Pubmed , Xenbase
Ruiz i Altaba, Sequential expression of HNF-3 beta and HNF-3 alpha by embryonic organizing centers: the dorsal lip/node, notochord and floor plate. 1993, Pubmed , Xenbase
Ruiz i Altaba, Pintallavis, a gene expressed in the organizer and midline cells of frog embryos: involvement in the development of the neural axis. 1992, Pubmed , Xenbase
Saha, A Xenopus homebox gene defines dorsal-ventral domains in the developing brain. 1993, Pubmed , Xenbase
Sampath, Induction of the zebrafish ventral brain and floorplate requires cyclops/nodal signalling. 1998, Pubmed , Xenbase
Sander, The opposing homeobox genes Goosecoid and Vent1/2 self-regulate Xenopus patterning. 2007, Pubmed , Xenbase
Sasai, Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes. 1994, Pubmed , Xenbase
Schäfer, Discontinuous organization and specification of the lateral floor plate in zebrafish. 2007, Pubmed
Schäfer, Hedgehog and retinoid signalling confines nkx2.2b expression to the lateral floor plate of the zebrafish trunk. 2005, Pubmed
Schäfer, Medial floor plate formation in zebrafish consists of two phases and requires trunk-derived Midkine-a. 2005, Pubmed
Schauerte, Sonic hedgehog is not required for the induction of medial floor plate cells in the zebrafish. 1998, Pubmed
Serafini, Netrin-1 is required for commissural axon guidance in the developing vertebrate nervous system. 1996, Pubmed
Smith, Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction. 1991, Pubmed , Xenbase
Sölter, Characterization of a subfamily of related winged helix genes, XFD-12/12'/12" (XFLIP), during Xenopus embryogenesis. 1999, Pubmed , Xenbase
Spemann, Induction of embryonic primordia by implantation of organizers from a different species. 1923. 2001, Pubmed
Strähle, one-eyed pinhead is required for development of the ventral midline of the zebrafish (Danio rerio) neural tube. 1997, Pubmed
Strähle, Vertebrate floor-plate specification: variations on common themes. 2004, Pubmed
Takabatake, Distinct expression of two types of Xenopus Patched genes during early embryogenesis and hindlimb development. 2000, Pubmed , Xenbase
Teillet, The relationships between notochord and floor plate in vertebrate development revisited. 1998, Pubmed
Tessier-Lavigne, Chemotropic guidance of developing axons in the mammalian central nervous system. , Pubmed
Tian, A temperature-sensitive mutation in the nodal-related gene cyclops reveals that the floor plate is induced during gastrulation in zebrafish. 2003, Pubmed
Varlet, nodal expression in the primitive endoderm is required for specification of the anterior axis during mouse gastrulation. 1997, Pubmed
von Dassow, Induction of the Xenopus organizer: expression and regulation of Xnot, a novel FGF and activin-regulated homeo box gene. 1993, Pubmed , Xenbase
Wettstein, The Xenopus homolog of Drosophila Suppressor of Hairless mediates Notch signaling during primary neurogenesis. 1997, Pubmed , Xenbase
Wijgerde, A direct requirement for Hedgehog signaling for normal specification of all ventral progenitor domains in the presumptive mammalian spinal cord. 2002, Pubmed , Xenbase
Wills, BMP antagonists and FGF signaling contribute to different domains of the neural plate in Xenopus. 2010, Pubmed , Xenbase
Yamada, Control of cell pattern in the developing nervous system: polarizing activity of the floor plate and notochord. 1991, Pubmed
Yamada, Control of cell pattern in the neural tube: motor neuron induction by diffusible factors from notochord and floor plate. 1993, Pubmed