Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Arranging the elements of the potassium channel: the T1 domain occludes the cytoplasmic face of the channel.
Varshney A
,
Chanda B
,
Mathew MK
.
???displayArticle.abstract???
The voltage-gated potassium channel is currently one of the few membrane proteins where functional roles have been mapped onto specific segments of sequence. Although high-resolution structures of the transmembrane portions of three bacterial potassium channels, the tetramerization domain and the cytoplasmic "ball" are available, their relative spatial arrangement in mammalian channels remains a matter of ongoing debate. Cryo-electron microscopic images of the six transmembrane voltage-gated Kv channel have been reconstructed at up to 18 A resolution, revealing that the T1 domain tetramerizes and is suspended below the transmembrane segments. However, the resolution of these images is insufficient to reveal the location of the third piece of the puzzle, the inactivating ball domain. We have used the aberrant interactions observed in a series of chimaeric channels to establish that an assembled T1 domain restricts access to the cytoplasmic face of the channel, suggesting that the N-terminal "ball and chain" may be confined in the space between the T1 domain and the transmembrane portion of the channel.
Aldrich,
Fifty years of inactivation.
2001, Pubmed
Aldrich,
Fifty years of inactivation.
2001,
Pubmed
Antz,
NMR structure of inactivation gates from mammalian voltage-dependent potassium channels.
1997,
Pubmed
Bezanilla,
The voltage sensor in voltage-dependent ion channels.
2000,
Pubmed
Bixby,
Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels.
1999,
Pubmed
Chanda,
Exploring the architecture of potassium channels using chimaeras to reveal signal transduction.
1999,
Pubmed
,
Xenbase
Chanda,
Transplanting the N-terminus from Kv1.4 to Kv1.1 generates an inwardly rectifying K+ channel.
1999,
Pubmed
,
Xenbase
Choe,
Towards the three-dimensional structure of voltage-gated potassium channels.
1999,
Pubmed
Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed
Gulbis,
Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent K+ channels.
2000,
Pubmed
,
Xenbase
Hopkins,
Interactions of snake dendrotoxins with potassium channels.
1999,
Pubmed
Jiang,
X-ray structure of a voltage-dependent K+ channel.
2003,
Pubmed
Jiang,
Crystal structure and mechanism of a calcium-gated potassium channel.
2002,
Pubmed
Jiang,
The principle of gating charge movement in a voltage-dependent K+ channel.
2003,
Pubmed
Kobertz,
Hanging gondola structure of the T1 domain in a voltage-gated K(+) channel.
2000,
Pubmed
,
Xenbase
Kobertz,
K+ channels lacking the 'tetramerization' domain: implications for pore structure.
1999,
Pubmed
,
Xenbase
Kreusch,
Crystal structure of the tetramerization domain of the Shaker potassium channel.
1998,
Pubmed
Kuo,
Crystal structure of the potassium channel KirBac1.1 in the closed state.
2003,
Pubmed
Latorre,
Structure and function of potassium channels in plants: some inferences about the molecular origin of inward rectification in KAT1 channels (Review).
2003,
Pubmed
MacKinnon,
Pore loops: an emerging theme in ion channel structure.
1995,
Pubmed
Männikkö,
Voltage-sensing mechanism is conserved among ion channels gated by opposite voltages.
2002,
Pubmed
,
Xenbase
Miller,
Conversion of a delayed rectifier K+ channel to a voltage-gated inward rectifier K+ channel by three amino acid substitutions.
1996,
Pubmed
Minor,
The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel.
2000,
Pubmed
,
Xenbase
Orlova,
Voltage-gated K+ channel from mammalian brain: 3D structure at 18A of the complete (alpha)4(beta)4 complex.
2003,
Pubmed
Ramaswami,
Human potassium channel genes: Molecular cloning and functional expression.
1990,
Pubmed
,
Xenbase
Rudy,
Contributions of Kv3 channels to neuronal excitability.
1999,
Pubmed
Sather,
Structural basis of ion channel permeation and selectivity.
1994,
Pubmed
Sesti,
Hyperpolarization moves S4 sensors inward to open MVP, a methanococcal voltage-gated potassium channel.
2003,
Pubmed
Shen,
Molecular recognition and assembly sequences involved in the subfamily-specific assembly of voltage-gated K+ channel subunit proteins.
1995,
Pubmed
Sigworth,
Structural biology: Life's transistors.
2003,
Pubmed
Smith,
The inward rectification mechanism of the HERG cardiac potassium channel.
1996,
Pubmed
Sokolova,
Three-dimensional structure of a voltage-gated potassium channel at 2.5 nm resolution.
2001,
Pubmed
Stephens,
Inactivation of the cloned potassium channel mouse Kv1.1 by the human Kv3.4 'ball' peptide and its chemical modification.
1995,
Pubmed
Stühmer,
Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain.
1989,
Pubmed
,
Xenbase
Tseng-Crank,
Functional role of the NH2-terminal cytoplasmic domain of a mammalian A-type K channel.
1993,
Pubmed
,
Xenbase
Varshney,
A tale of two tails: cytosolic termini and K(+) channel function.
2003,
Pubmed
Varshney,
Inward and outward potassium currents through the same chimeric human Kv channel.
2003,
Pubmed
,
Xenbase
Varshney,
Modulation of voltage sensitivity by N-terminal cytoplasmic residues in human Kv1.2 channels.
2002,
Pubmed
,
Xenbase
Wissmann,
NMR structure and functional characteristics of the hydrophilic N terminus of the potassium channel beta-subunit Kvbeta1.1.
1999,
Pubmed
,
Xenbase
Wissmann,
Solution structure and function of the "tandem inactivation domain" of the neuronal A-type potassium channel Kv1.4.
2003,
Pubmed
Xu,
Assembly of voltage-gated potassium channels. Conserved hydrophilic motifs determine subfamily-specific interactions between the alpha-subunits.
1995,
Pubmed
Yellen,
The moving parts of voltage-gated ion channels.
1998,
Pubmed
Zagotta,
Restoration of inactivation in mutants of Shaker potassium channels by a peptide derived from ShB.
1990,
Pubmed
,
Xenbase
Zhou,
Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors.
2001,
Pubmed
,
Xenbase
