Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
In Vitro Cell Dev Biol Anim
2011 Aug 01;477:470-83. doi: 10.1007/s11626-011-9423-6.
Show Gene links
Show Anatomy links
Adult-type myogenesis of the frog Xenopus laevis specifically suppressed by notochord cells but promoted by spinal cord cells in vitro.
Yamane H
,
Ihara S
,
Kuroda M
,
Nishikawa A
.
???displayArticle.abstract???
Larval-to-adult myogenic conversion occurs in the dorsal muscle but not in the tailmuscle during Xenopus laevis metamorphosis. To know the mechanism for tail-specific suppression of adult myogenesis, response character was compared between adult myogenic cells (Ad-cells) and larval tail myogenic cells (La-cells) to a Sonic hedgehog (Shh) inhibitor, notochord (Nc) cells, and spinal cord (SC) cells in vitro. Cyclopamine, an Shh inhibitor, suppressed the differentiation of cultured Ad (but not La) cells, suggesting the significance of Shh signaling in promoting adult myogenesis. To test the possibility that Shh-producing axial elements (notochord and spinal cord) regulate adult myogenesis, Ad-cells or La-cells were co-cultured with Nc or SC cells. The results showed that differentiation of Ad-cells were strongly inhibited by Nc cells but promoted by SC cells. If Ad-cells were "separately" co-cultured with Nc cells without direct cell-cell interactions, adult differentiation was not inhibited but rather promoted, suggesting that Nc cells have two roles, one is a short-range suppression and another is a long-range promotion for adult myogenesis. Immunohistochemical analysis showed both notochord and spinal cord express the N-terminal Shh fragment throughout metamorphosis. The "spinal cord-promotion" and long-range effect by Nc cells on adult myogenesis is thus involved in Shh signaling, while the signaling concerning the short-range "Nc suppression" will be determined by future studies. Interestingly, these effects, "Nc suppression" and "SC promotion" were not observed for La-cells. Situation where the spinal cord/notochord cross-sectional ratio is quite larger in tadpoletrunk than in the tail seems to contribute to trunk-specific promotion and tail-specific suppression of adult myogenesis during Xenopus metamorphosis.
Blagden,
Notochord induction of zebrafish slow muscle mediated by Sonic hedgehog.
1997, Pubmed
Blagden,
Notochord induction of zebrafish slow muscle mediated by Sonic hedgehog.
1997,
Pubmed
Borycki,
Sonic hedgehog controls epaxial muscle determination through Myf5 activation.
1999,
Pubmed
Das,
Identification of direct thyroid hormone response genes reveals the earliest gene regulation programs during frog metamorphosis.
2009,
Pubmed
,
Xenbase
Ekker,
Distinct expression and shared activities of members of the hedgehog gene family of Xenopus laevis.
1995,
Pubmed
,
Xenbase
Elia,
Sonic hedgehog promotes proliferation and differentiation of adult muscle cells: Involvement of MAPK/ERK and PI3K/Akt pathways.
2007,
Pubmed
Gustafsson,
Myf5 is a direct target of long-range Shh signaling and Gli regulation for muscle specification.
2002,
Pubmed
Hebrok,
Notochord repression of endodermal Sonic hedgehog permits pancreas development.
1998,
Pubmed
Incardona,
The teratogenic Veratrum alkaloid cyclopamine inhibits sonic hedgehog signal transduction.
1998,
Pubmed
Ishizuya-Oka,
Thyroid hormone-induced expression of sonic hedgehog correlates with adult epithelial development during remodeling of the Xenopus stomach and intestine.
2001,
Pubmed
,
Xenbase
Ishizuya-Oka,
Requirement for matrix metalloproteinase stromelysin-3 in cell migration and apoptosis during tissue remodeling in Xenopus laevis.
2000,
Pubmed
,
Xenbase
Ishizuya-Oka,
Apoptosis and cell proliferation in the Xenopus small intestine during metamorphosis.
1996,
Pubmed
,
Xenbase
Kawakami,
Regulation of desmin expression in adult-type myogenesis and muscle maturation during Xenopus laevis metamorphosis.
2009,
Pubmed
,
Xenbase
Kerr,
An electron-microscope study of cell deletion in the anuran tadpole tail during spontaneous metamorphosis with special reference to apoptosis of striated muscle fibers.
1974,
Pubmed
Martin,
Hedgehog signaling regulates the amount of hypaxial muscle development during Xenopus myogenesis.
2007,
Pubmed
,
Xenbase
Mukhi,
Gene switching at Xenopus laevis metamorphosis.
2010,
Pubmed
,
Xenbase
Münsterberg,
Combinatorial signals from the neural tube, floor plate and notochord induce myogenic bHLH gene expression in the somite.
1995,
Pubmed
Muntz,
Myogenesis in the trunk and leg during development of the tadpole of Xenopus laevis (Daudin 1802).
1975,
Pubmed
,
Xenbase
Nishikawa,
Isoform transition of contractile proteins related to muscle remodeling with an axial gradient during metamorphosis in Xenopus laevis.
1994,
Pubmed
,
Xenbase
Nishikawa,
Spatial, temporal and hormonal regulation of programmed muscle cell death during metamorphosis of the frog Xenopus laevis.
1995,
Pubmed
,
Xenbase
Nishikawa,
[Induction of cell differentiation and programmed cell death in amphibian metamorphosis].
1997,
Pubmed
,
Xenbase
Ochi,
Hhip regulates zebrafish muscle development by both sequestering Hedgehog and modulating localization of Smoothened.
2006,
Pubmed
Placzek,
The role of the notochord and floor plate in inductive interactions.
1995,
Pubmed
Schreiber,
Cell-cell interactions during remodeling of the intestine at metamorphosis in Xenopus laevis.
2009,
Pubmed
,
Xenbase
Shibota,
Larval-to-adult conversion of a myogenic system in the frog, Xenopus laevis, by larval-type myoblast-specific control of cell division, cell differentiation, and programmed cell death by triiodo-L-thyronine.
2000,
Pubmed
,
Xenbase
Shimizu-Nishikawa,
Regulation of specific developmental fates of larval- and adult-type muscles during metamorphosis of the frog Xenopus.
2002,
Pubmed
,
Xenbase
Stern,
Neural tube and notochord promote in vitro myogenesis in single somite explants.
1995,
Pubmed
Zeng,
A freely diffusible form of Sonic hedgehog mediates long-range signalling.
2001,
Pubmed
