Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Struct Biol
2012 Jan 01;1771:81-9. doi: 10.1016/j.jsb.2011.11.004.
Show Gene links
Show Anatomy links
Domain topology of nucleoporin Nup98 within the nuclear pore complex.
Chatel G
,
Desai SH
,
Mattheyses AL
,
Powers MA
,
Fahrenkrog B
.
???displayArticle.abstract???
Nuclear pore complexes (NPCs) facilitate selective transport of macromolecules across the nuclear envelope in interphase eukaryotic cells. NPCs are composed of roughly 30 different proteins (nucleoporins) of which about one third are characterized by the presence of phenylalanine-glycine (FG) repeat domains that allow the association of soluble nuclear transport receptors with the NPC. Two types of FG (FG/FxFG and FG/GLFG) domains are found in nucleoporins and Nup98 is the sole vertebrate nucleoporin harboring the GLFG-type repeats. By immuno-electron microscopy using isolated nuclei from Xenopus oocytes we show here the localization of distinct domains of Nup98. We examined the localization of the C- and N-terminal domain of Nup98 by immunogold-labeling using domain-specific antibodies against Nup98 and by expressing epitope tagged versions of Nup98. Our studies revealed that anchorage of Nup98 to NPCs through its C-terminal autoproteolytic domain occurs in the center of the NPC, whereas its N-terminal GLFG domain is more flexible and is detected at multiple locations within the NPC. Additionally, we have confirmed the central localization of Nup98 within the NPC using super resolution structured illumination fluorescence microscopy (SIM) to position Nup98 domains relative to markers of cytoplasmic filaments and the nuclear basket. Our data support the notion that Nup98 is a major determinant of the permeability barrier of NPCs.
Arnaoutov,
Crm1 is a mitotic effector of Ran-GTP in somatic cells.
2005, Pubmed
Arnaoutov,
Crm1 is a mitotic effector of Ran-GTP in somatic cells.
2005,
Pubmed
Bayliss,
Structural basis for the interaction between FxFG nucleoporin repeats and importin-beta in nuclear trafficking.
2000,
Pubmed
Beck,
Snapshots of nuclear pore complexes in action captured by cryo-electron tomography.
2007,
Pubmed
Blevins,
Complex formation among the RNA export proteins Nup98, Rae1/Gle2, and TAP.
2003,
Pubmed
,
Xenbase
Capelson,
Chromatin-bound nuclear pore components regulate gene expression in higher eukaryotes.
2010,
Pubmed
Cross,
Nup98 regulates bipolar spindle assembly through association with microtubules and opposition of MCAK.
2011,
Pubmed
,
Xenbase
D'Angelo,
Structure, dynamics and function of nuclear pore complexes.
2008,
Pubmed
Denning,
Disorder in the nuclear pore complex: the FG repeat regions of nucleoporins are natively unfolded.
2003,
Pubmed
Dultz,
Systematic kinetic analysis of mitotic dis- and reassembly of the nuclear pore in living cells.
2008,
Pubmed
Fahrenkrog,
Domain-specific antibodies reveal multiple-site topology of Nup153 within the nuclear pore complex.
2002,
Pubmed
,
Xenbase
Fahrenkrog,
The nuclear pore complex: nucleocytoplasmic transport and beyond.
2003,
Pubmed
Feldherr,
The location of the transport gate in the nuclear pore complex.
1997,
Pubmed
,
Xenbase
Fiserova,
Nuclear envelope and nuclear pore complex structure and organization in tobacco BY-2 cells.
2009,
Pubmed
Fiserova,
Facilitated transport and diffusion take distinct spatial routes through the nuclear pore complex.
2010,
Pubmed
Fontoura,
A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96.
1999,
Pubmed
Fontoura,
The nucleoporin Nup98 is a site for GDP/GTP exchange on ran and termination of karyopherin beta 2-mediated nuclear import.
2000,
Pubmed
Frenkiel-Krispin,
Structural analysis of a metazoan nuclear pore complex reveals a fused concentric ring architecture.
2010,
Pubmed
,
Xenbase
Fried,
Nucleocytoplasmic transport: taking an inventory.
2003,
Pubmed
Frosst,
Tpr is localized within the nuclear basket of the pore complex and has a role in nuclear protein export.
2002,
Pubmed
Fukuhara,
Specific monoclonal antibody against the nuclear pore complex protein, nup98.
2005,
Pubmed
Griffis,
Nup98 is a mobile nucleoporin with transcription-dependent dynamics.
2002,
Pubmed
,
Xenbase
Griffis,
Nup98 localizes to both nuclear and cytoplasmic sides of the nuclear pore and binds to two distinct nucleoporin subcomplexes.
2003,
Pubmed
Griffis,
Distinct functional domains within nucleoporins Nup153 and Nup98 mediate transcription-dependent mobility.
2004,
Pubmed
Gustafsson,
Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination.
2008,
Pubmed
Harel,
Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores.
2003,
Pubmed
,
Xenbase
Hodel,
The three-dimensional structure of the autoproteolytic, nuclear pore-targeting domain of the human nucleoporin Nup98.
2002,
Pubmed
Jeganathan,
The Rae1-Nup98 complex prevents aneuploidy by inhibiting securin degradation.
2005,
Pubmed
Kalverda,
Nucleoporins directly stimulate expression of developmental and cell-cycle genes inside the nucleoplasm.
2010,
Pubmed
Keminer,
Optical recording of signal-mediated protein transport through single nuclear pore complexes.
1999,
Pubmed
,
Xenbase
Kowalczyk,
Single-molecule transport across an individual biomimetic nuclear pore complex.
2011,
Pubmed
Laurell,
Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry.
2011,
Pubmed
Lim,
The nuclear pore complex up close.
2006,
Pubmed
Lim,
Towards reconciling structure and function in the nuclear pore complex.
2008,
Pubmed
Lussi,
The nucleoporin Nup88 is interacting with nuclear lamin A.
2011,
Pubmed
,
Xenbase
Mohr,
Characterisation of the passive permeability barrier of nuclear pore complexes.
2009,
Pubmed
Naim,
Passive and facilitated transport in nuclear pore complexes is largely uncoupled.
2007,
Pubmed
Oka,
The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export.
2010,
Pubmed
Panté,
Interactions and three-dimensional localization of a group of nuclear pore complex proteins.
1994,
Pubmed
,
Xenbase
Park,
Differential targeting of nuclear pore complex proteins in poliovirus-infected cells.
2008,
Pubmed
Patel,
Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex.
2007,
Pubmed
Paulillo,
Nucleoporin domain topology is linked to the transport status of the nuclear pore complex.
2005,
Pubmed
,
Xenbase
Paulillo,
Changes in nucleoporin domain topology in response to chemical effectors.
2006,
Pubmed
,
Xenbase
Powers,
Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication.
1995,
Pubmed
,
Xenbase
Powers,
The vertebrate GLFG nucleoporin, Nup98, is an essential component of multiple RNA export pathways.
1997,
Pubmed
,
Xenbase
Pritchard,
RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains.
1999,
Pubmed
,
Xenbase
Radu,
The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex.
1995,
Pubmed
Rosenblum,
Autoproteolysis in nucleoporin biogenesis.
1999,
Pubmed
Schwartz,
Modularity within the architecture of the nuclear pore complex.
2005,
Pubmed
Schwarz-Herion,
Domain topology of the p62 complex within the 3-D architecture of the nuclear pore complex.
2007,
Pubmed
,
Xenbase
Slot,
A new method of preparing gold probes for multiple-labeling cytochemistry.
1985,
Pubmed
Terry,
Nuclear mRNA export requires specific FG nucleoporins for translocation through the nuclear pore complex.
2007,
Pubmed
Vasu,
Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export.
2001,
Pubmed
,
Xenbase
Wälde,
The nucleoporin Nup358/RanBP2 promotes nuclear import in a cargo- and transport receptor-specific manner.
2012,
Pubmed
Wälde,
The Part and the Whole: functions of nucleoporins in nucleocytoplasmic transport.
2010,
Pubmed
Walther,
The conserved Nup107-160 complex is critical for nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Yamada,
A bimodal distribution of two distinct categories of intrinsically disordered structures with separate functions in FG nucleoporins.
2010,
Pubmed
Yoshida,
Structural and functional analysis of an essential nucleoporin heterotrimer on the cytoplasmic face of the nuclear pore complex.
2011,
Pubmed
Zolotukhin,
Nucleoporins nup98 and nup214 participate in nuclear export of human immunodeficiency virus type 1 Rev.
1999,
Pubmed
,
Xenbase
