Drug Metab Pharmacokinet 2012 Jan 01;273:286-93. doi: 10.2133/dmpk.dmpk-11-rg-076.

Functional impairment of rat taurine transporter by activation of nitrogen oxide through superoxide.

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The objective of this study was to identify the nitrogen oxide form(s) involved in the functional impairment of the rat taurine transport system. Taurine uptake activity in the rat renal brush border membrane vesicle (RBBMV) preparation or Xenopus laevis oocytes that express the rat taurine transporter was compared after the pretreatment with nitrogen oxide donors from which nitric oxide (NO) is released at different rates. The functional impairment was associated with a reduced Vmax, but did not involve an alteration in the Km, of taurine uptake in the RBBMV preparation that had been pretreated with sodium nitroprusside, a slow release nitric oxide (NO) donor. When the preparation was pretreated with S-nitroso-N-acetyl penicillamine, a rapid release NO donor, the activity of taurine uptake was unaffected. The activity was not statistically different from the control after the pretreatment with sodium nitroprusside and superoxide dismutase. Consistent with the study with RBBMV, a similar alteration in the activity of taurine uptake by NO donors was observed in oocytes expressing the transporter. Considering the fact that peroxynitrite, a highly reactive nitrogen oxide form, is formed by the reaction between NO and superoxide, the taurine transporter, and probably other transport systems as well, may be functionally impaired by peroxynitrite.

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