XB-ART-44606
Dev Cell
2011 Sep 13;213:410-9. doi: 10.1016/j.devcel.2011.07.018.
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And the dead shall rise: actin and myosin return to the spindle.
Sandquist JC
,
Kita AM
,
Bement WM
.
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The spindle directs chromosome partitioning in eukaryotes and, for the last three decades, has been considered primarily a structure based on microtubules, microtubule motors, and other microtubule binding proteins. However, a surprisingly large body of both old and new studies suggests roles for actin filaments (F-actin) and myosins (F-actin-based motor proteins) in spindle assembly and function. Here we review these data and conclude that in several cases the evidence for the participation of F-actin and myosins in spindle function is very strong, and in the situations where it is less strong, there is nevertheless enough evidence to warrant further investigation.
???displayArticle.pubmedLink??? 21920311
???displayArticle.pmcLink??? PMC3197778
???displayArticle.link??? Dev Cell
???displayArticle.grants??? [+]
5F32GM090674 NIGMS NIH HHS , GM052932 NIGMS NIH HHS , F32 GM090674-02 NIGMS NIH HHS , R01 GM052932-14 NIGMS NIH HHS , R01 GM052932 NIGMS NIH HHS , F32 GM090674 NIGMS NIH HHS
Species referenced: Xenopus
Genes referenced: actl6a
References [+] :
Aubin,
Analysis of actin and microfilament-associated proteins in the mitotic spindle and cleavage furrow of PtK2 cells by immunofluorescence microscopy. A critical note.
1979, Pubmed
Aubin, Analysis of actin and microfilament-associated proteins in the mitotic spindle and cleavage furrow of PtK2 cells by immunofluorescence microscopy. A critical note. 1979, Pubmed
Azoury, Symmetry breaking in mouse oocytes requires transient F-actin meshwork destabilization. 2011, Pubmed
Azoury, Spindle positioning in mouse oocytes relies on a dynamic meshwork of actin filaments. 2008, Pubmed
Ballestrem, Actin dynamics in living mammalian cells. 1998, Pubmed
Barak, Differential staining of actin in metaphase spindles with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin and fluorescent DNase: is actin involved in chromosomal movement? 1981, Pubmed
Berg, Myosin-X is an unconventional myosin that undergoes intrafilopodial motility. 2002, Pubmed
Burkel, Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin. 2007, Pubmed , Xenbase
Cande, A comparison of the distribution of actin and tubulin in the mammalian mitotic spindle as seen by indirect immunofluorescence. 1977, Pubmed
Cao, Cortical actin dynamics facilitate early-stage centrosome separation. 2010, Pubmed
Carreno, Moesin and its activating kinase Slik are required for cortical stability and microtubule organization in mitotic cells. 2008, Pubmed
Castagnetti, Fission yeast cells undergo nuclear division in the absence of spindle microtubules. 2010, Pubmed
Chiroli, Cdc14 inhibition by the spindle assembly checkpoint prevents unscheduled centrosome separation in budding yeast. 2009, Pubmed
Doyle, Fission yeast Myo51 is a meiotic spindle pole body component with discrete roles during cell fusion and spore formation. 2009, Pubmed
Dulyaninova, The N-terminus of the long MLCK induces a disruption in normal spindle morphology and metaphase arrest. 2004, Pubmed
Edwards, GFP-moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila. 1997, Pubmed
Fabian, Possible roles of actin and myosin during anaphase chromosome movements in locust spermatocytes. 2007, Pubmed
Fabritius, Nuclear and spindle positioning during oocyte meiosis. 2011, Pubmed
Field, Actin behavior in bulk cytoplasm is cell cycle regulated in early vertebrate embryos. 2011, Pubmed , Xenbase
Fielding, Integrin-linked kinase localizes to the centrosome and regulates mitotic spindle organization. 2008, Pubmed
Fink, External forces control mitotic spindle positioning. 2011, Pubmed
Forer, Actin in spindles of Haemanthus katherinae endosperm. I. General results using various glycerination methods. 1979, Pubmed
Fujiwara, Fluorescent antibody localization of myosin in the cytoplasm, cleavage furrow, and mitotic spindle of human cells. 1976, Pubmed
Gachet, Mechanism controlling perpendicular alignment of the spindle to the axis of cell division in fission yeast. 2004, Pubmed
Gachet, A MAP kinase-dependent actin checkpoint ensures proper spindle orientation in fission yeast. 2001, Pubmed
Gard, F-actin is required for spindle anchoring and rotation in Xenopus oocytes: a re-examination of the effects of cytochalasin B on oocyte maturation. 1995, Pubmed , Xenbase
Gatlin, Microtubule motors in eukaryotic spindle assembly and maintenance. 2010, Pubmed
Gawadi, Actin in the mitotic spindle. 1971, Pubmed
Goshima, Control of mitotic spindle length. 2010, Pubmed
Harvey, Conservation of mechanisms controlling entry into mitosis: budding yeast wee1 delays entry into mitosis and is required for cell size control. 2003, Pubmed
Heil-Chapdelaine, Dynein-dependent movements of the mitotic spindle in Saccharomyces cerevisiae Do not require filamentous actin. 2000, Pubmed
Herman, Comparison of purified anti-actin and fluorescent-heavy meromyosin staining patterns in dividing cells. 1979, Pubmed
Hirano, Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain. 2011, Pubmed
Hirota, Zyxin, a regulator of actin filament assembly, targets the mitotic apparatus by interacting with h-warts/LATS1 tumor suppressor. 2000, Pubmed
Hwang, Spindle orientation in Saccharomyces cerevisiae depends on the transport of microtubule ends along polarized actin cables. 2003, Pubmed
Johansen, Do nuclear envelope and intranuclear proteins reorganize during mitosis to form an elastic, hydrogel-like spindle matrix? 2011, Pubmed
Kaji, LIM kinase-mediated cofilin phosphorylation during mitosis is required for precise spindle positioning. 2008, Pubmed
Kelley, Xenopus nonmuscle myosin heavy chain isoforms have different subcellular localizations and enzymatic activities. 1996, Pubmed , Xenbase
Kiehart, Evidence that myosin does not contribute to force production in chromosome movement. 1982, Pubmed
Komatsu, Effects of the regulatory light chain phosphorylation of myosin II on mitosis and cytokinesis of mammalian cells. 2000, Pubmed
Kovar, Control of the assembly of ATP- and ADP-actin by formins and profilin. 2006, Pubmed
Kunda, Moesin controls cortical rigidity, cell rounding, and spindle morphogenesis during mitosis. 2008, Pubmed
Kwon, Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes. 2008, Pubmed
Lee, Control of mitotic spindle position by the Saccharomyces cerevisiae formin Bni1p. 1999, Pubmed
Lénárt, A contractile nuclear actin network drives chromosome congression in oocytes. 2005, Pubmed
Li, Actin-driven chromosomal motility leads to symmetry breaking in mammalian meiotic oocytes. 2008, Pubmed
Lohka, Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts. 1985, Pubmed , Xenbase
Luxenburg, Developmental roles for Srf, cortical cytoskeleton and cell shape in epidermal spindle orientation. 2011, Pubmed
Ma, Ablation of nonmuscle myosin II-B and II-C reveals a role for nonmuscle myosin II in cardiac myocyte karyokinesis. 2010, Pubmed
Mao, Planar polarization of the atypical myosin Dachs orients cell divisions in Drosophila. 2011, Pubmed
Maresca, Welcome to a new kind of tension: translating kinetochore mechanics into a wait-anaphase signal. 2010, Pubmed
Maro, Changes in actin distribution during fertilization of the mouse egg. 1984, Pubmed
Matsumura, Specific localization of serine 19 phosphorylated myosin II during cell locomotion and mitosis of cultured cells. 1998, Pubmed
Mattagajasingh, A nonerythroid isoform of protein 4.1R interacts with the nuclear mitotic apparatus (NuMA) protein. 1999, Pubmed
Maxwell, RHAMM is a centrosomal protein that interacts with dynein and maintains spindle pole stability. 2003, Pubmed , Xenbase
McMillan, A morphogenesis checkpoint monitors the actin cytoskeleton in yeast. 1998, Pubmed
Meadows, Latrunculin A delays anaphase onset in fission yeast by disrupting an Ase1-independent pathway controlling mitotic spindle stability. 2008, Pubmed
Mitsushima, Revolving movement of a dynamic cluster of actin filaments during mitosis. 2010, Pubmed
Moore, Coordinating mitosis with cell polarity: Molecular motors at the cell cortex. 2010, Pubmed
Mori, Intracellular transport by an anchored homogeneously contracting F-actin meshwork. 2011, Pubmed
Palmer, Role of astral microtubules and actin in spindle orientation and migration in the budding yeast, Saccharomyces cerevisiae. 1992, Pubmed
Park, Role of focal adhesion kinase Ser-732 phosphorylation in centrosome function during mitosis. 2009, Pubmed
Petritsch, The Drosophila myosin VI Jaguar is required for basal protein targeting and correct spindle orientation in mitotic neuroblasts. 2003, Pubmed
Pfender, Spire-type actin nucleators cooperate with Formin-2 to drive asymmetric oocyte division. 2011, Pubmed
Pruyne, Stable and dynamic axes of polarity use distinct formin isoforms in budding yeast. 2004, Pubmed
Pruyne, Tropomyosin-containing actin cables direct the Myo2p-dependent polarized delivery of secretory vesicles in budding yeast. 1998, Pubmed
Rajagopalan, A potential tension-sensing mechanism that ensures timely anaphase onset upon metaphase spindle orientation. 2004, Pubmed
Raskin, Rapid pole-to-pole oscillation of a protein required for directing division to the middle of Escherichia coli. 1999, Pubmed
Riedl, Lifeact: a versatile marker to visualize F-actin. 2008, Pubmed
Rosenblatt, Myosin II-dependent cortical movement is required for centrosome separation and positioning during mitotic spindle assembly. 2004, Pubmed
Rump, Myosin-1C associates with microtubules and stabilizes the mitotic spindle during cell division. 2011, Pubmed
Sampson, Phallacidin stains the kinetochore region in the mitotic spindle of the green algae Oedogonium spp. 2001, Pubmed
Sanger, Presence of actin during chromosomal movement. 1975, Pubmed
Schloss, Myosin subfragment binding for the localization of actin-like microfilaments in cultured cells. A light and electron microscope study. 1977, Pubmed
Schmit, Characterization and dynamics of cytoplasmic F-actin in higher plant endosperm cells during interphase, mitosis, and cytokinesis. 1987, Pubmed
Schuh, A new model for asymmetric spindle positioning in mouse oocytes. 2008, Pubmed
Seagull, Microfilaments: dynamic arrays in higher plant cells. 1987, Pubmed
Sia, Control of Swe1p degradation by the morphogenesis checkpoint. 1998, Pubmed
Silverman-Gavrila, Evidence that actin and myosin are involved in the poleward flux of tubulin in metaphase kinetochore microtubules of crane-fly spermatocytes. 2000, Pubmed
Simerly, Differential expression and functions of cortical myosin IIA and IIB isotypes during meiotic maturation, fertilization, and mitosis in mouse oocytes and embryos. 1998, Pubmed
Snyder, Cytochalasin J affects chromosome congression and spindle microtubule organization in PtK1 cells. 1995, Pubmed
Sommi, Actomyosin-dependent cortical dynamics contributes to the prophase force-balance in the early Drosophila embryo. 2011, Pubmed
Staiger, Microfilament Distribution in Maize Meiotic Mutants Correlates with Microtubule Organization. 1991, Pubmed
Sumi, Different activity regulation and subcellular localization of LIMK1 and LIMK2 during cell cycle transition. 2006, Pubmed
Theesfeld, The role of actin in spindle orientation changes during the Saccharomyces cerevisiae cell cycle. 1999, Pubmed
Théry, The extracellular matrix guides the orientation of the cell division axis. 2005, Pubmed
Tournier, Disruption of astral microtubule contact with the cell cortex activates a Bub1, Bub3, and Mad3-dependent checkpoint in fission yeast. 2004, Pubmed
Toyoshima, Integrin-mediated adhesion orients the spindle parallel to the substratum in an EB1- and myosin X-dependent manner. 2007, Pubmed
Traas, An actin network is present in the cytoplasm throughout the cell cycle of carrot cells and associates with the dividing nucleus. 1987, Pubmed
Van Blerkom, Regulation of development in the fully grown mouse oocyte: chromosome-mediated temporal and spatial differentiation of the cytoplasm and plasma membrane. 1986, Pubmed
Visa, Nuclear functions of actin. 2010, Pubmed
Wang, Centrosome separation driven by actin-microfilaments during mitosis is mediated by centrosome-associated tyrosine-phosphorylated cortactin. 2008, Pubmed
Waterman-Storer, Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells. 1998, Pubmed , Xenbase
Weber, A microtubule-binding myosin required for nuclear anchoring and spindle assembly. 2004, Pubmed , Xenbase
Woolner, Myosin-10 and actin filaments are essential for mitotic spindle function. 2008, Pubmed , Xenbase
Wu, Counting cytokinesis proteins globally and locally in fission yeast. 2005, Pubmed
Wu, Myosin Va associates with microtubule-rich domains in both interphase and dividing cells. 1998, Pubmed
Wühr, Mitosis: new roles for myosin-X and actin at the spindle. 2008, Pubmed , Xenbase
Xie, Jasplakinolide, an actin stabilizing agent, alters anaphase chromosome movements in crane-fly spermatocytes. 2008, Pubmed
Yasuda, Localization of actin filaments on mitotic apparatus in tobacco BY-2 cells. 2005, Pubmed
Yin, Myosin V orientates the mitotic spindle in yeast. 2000, Pubmed
Yoo, Differential regulation of protrusion and polarity by PI3K during neutrophil motility in live zebrafish. 2010, Pubmed
Yu, Visualization of actin cytoskeletal dynamics during the cell cycle in tobacco (Nicotiana tabacum L. cv Bright Yellow) cells. 2006, Pubmed