Vaccaro MC (2003), Sequencing and characterization of the Xenopus ...

XB-ART-4487

Gene 2003 Oct 30;318:163-7. doi: 10.1016/s0378-1119(03)00771-6.

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Sequencing and characterization of the Xenopus laevis ribosomal protein L34 cDNA.

Vaccaro MC .

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In this paper, a cDNA homologous to the mammalian ribosomal protein (r-protein) L34 was isolated from a Xenopus laevis oocytes library and named XL34. It encodes a protein of 116 residues with an Mr of 13.2 kDa and a highly basic sequence. The nucleotide (nt) and deduced amino acid (aa) sequence have been compared with the L34 sequence from other species. This analysis showed that the L34 is a protein greatly conserved from prokaryotes to eukaryotes.XL34 mRNA is abundantly present in the whole cytoplasm of oocytes at stages 1 and 2.

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Species referenced: Xenopus laevis
Genes referenced: rpl34

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	Fig. 4. The Northern blot analysis on total RNA forms stages 1 and 2 previtellogenic oocytes (lane A) and vitellogenic oocytes (lane B) with the cDNA XL34 as probe (upper panel). The same filter was reprobed with histone H2 cDNA as control (lower panel). The size was determined with 0.24â€“9.5 Kb RNA ladder (Invitrogen).
	Fig. 5. In situ hybridization on sections of paraffin-embedded oocytes with XL34 antisense and sense riboprobe. The signal is evident in stages 1 and 2 oocytes (a). In oocytes at stage 3, the signal decreases (b). Section hybridized with sense riboprobe as negative control (c). The bar indicates the unit length: 100 Î¼m.
	rpl34 (ribosomal protein L34 ) gene expression in Xenopus laevis oocytes, stage 1 and 2.