Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Physiol
2012 Aug 01;59015:3597-609. doi: 10.1113/jphysiol.2012.232405.
Show Gene links
Show Anatomy links
Mitochondrial activation at the onset of contractions in isolated myofibres during successive contractile periods.
Gandra PG
,
Nogueira L
,
Hogan MC
.
???displayArticle.abstract???
At the onset of skeletal muscle repetitive contractions, there is a significant delay in the time to achieve oxidative phosphorylation steady state. The purpose of the present study was to examine the factors that limit oxidative phosphorylation at the onset of contractions. NAD(P)H was measured in real time during two contractile periods (2 min each) separated by 5 min of rest in intact single muscle fibres (n = 7) isolated from Xenopus laevis. The fibres were then loaded with the dye tetramethylrhodamine methyl ester perchlorate (TMRM) to evaluate the kinetics of the mitochondrial membrane potential (Δψ (m)) during two further successive contractile periods. At the onset of contractions in the first period, NAD(P)H exhibited a time delay (14.1 ± 1.3 s) before decreasing toward a steady state. In contrast, Δψ(m) decreased immediately after the first contraction and started to be reestablished after 10.7 ± 0.9 s, with restoration to the pre-stimulation values after approximately 32 s. In the second contractile period (5 min after the first), NAD(P)H decreased immediately (i.e. no time delay) after the first contraction and had a significantly shorter time constant compared to the first contractile bout (3.3 ± 0.3 vs. 5.0 ± 0.2 s, P < 0.05). During the second bout, Δψ(m) remained unchanged from pre-stimulation values. These results suggest: (1) that at the onset of contractions, oxidative phosphorylation is primarily limited by the activity of the electron transport chain complexes rather than by a limited level of substrates; and (2) when the muscle is 'primed' by previous contractile activity, the faster enhancement of the cellular respiratory rate is due to intrinsic factors within the myofibre.
Balaban,
Domestication of the cardiac mitochondrion for energy conversion.
2009, Pubmed
Balaban,
Domestication of the cardiac mitochondrion for energy conversion.
2009,
Pubmed
Bangsbo,
Muscle oxygen kinetics at onset of intense dynamic exercise in humans.
2000,
Pubmed
Bangsbo,
Enhanced pyruvate dehydrogenase activity does not affect muscle O2 uptake at onset of intense exercise in humans.
2002,
Pubmed
Bowen,
The intramuscular contribution to the slow oxygen uptake kinetics during exercise in chronic heart failure is related to the severity of the condition.
2012,
Pubmed
Brand,
Assessing mitochondrial dysfunction in cells.
2011,
Pubmed
Campbell-O'Sullivan,
Low intensity exercise in humans accelerates mitochondrial ATP production and pulmonary oxygen kinetics during subsequent more intense exercise.
2002,
Pubmed
Chance,
Skeletal muscle energetics with PNMR: personal views and historic perspectives.
2006,
Pubmed
CHANCE,
Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization.
1955,
Pubmed
Goodwin,
VO(2) on-kinetics in isolated canine muscle in situ during slowed convective O(2) delivery.
2012,
Pubmed
Grassi,
Role of convective O(2) delivery in determining VO(2) on-kinetics in canine muscle contracting at peak VO(2).
2000,
Pubmed
Grassi,
Oxygen uptake on-kinetics in dog gastrocnemius in situ following activation of pyruvate dehydrogenase by dichloroacetate.
2002,
Pubmed
Grassi,
Muscle O2 uptake kinetics in humans: implications for metabolic control.
1996,
Pubmed
Grassi,
Faster adjustment of O2 delivery does not affect V(O2) on-kinetics in isolated in situ canine muscle.
1998,
Pubmed
Grassi,
Peripheral O2 diffusion does not affect V(O2)on-kinetics in isolated insitu canine muscle.
1998,
Pubmed
Gurd,
Prior heavy exercise elevates pyruvate dehydrogenase activity and speeds O2 uptake kinetics during subsequent moderate-intensity exercise in healthy young adults.
2006,
Pubmed
Hernández,
A prior bout of contractions speeds VO2 and blood flow on-kinetics and reduces the VO2 slow-component amplitude in canine skeletal muscle contracting in situ.
2010,
Pubmed
Hogan,
NAD(P)H fluorescence imaging of mitochondrial metabolism in contracting Xenopus skeletal muscle fibers: effect of oxygen availability.
2005,
Pubmed
,
Xenbase
Hogan,
Fall in intracellular PO(2) at the onset of contractions in Xenopus single skeletal muscle fibers.
2001,
Pubmed
,
Xenbase
Hopper,
Mitochondrial matrix phosphoproteome: effect of extra mitochondrial calcium.
2006,
Pubmed
Howlett,
Intracellular PO(2) decreases with increasing stimulation frequency in contracting single Xenopus muscle fibers.
2001,
Pubmed
,
Xenbase
Howlett,
Dichloroacetate accelerates the fall in intracellular PO2 at onset of contractions in Xenopus single muscle fibers.
2003,
Pubmed
,
Xenbase
Hüttemann,
Regulation of mitochondrial oxidative phosphorylation through cell signaling.
2007,
Pubmed
Hüttemann,
Regulation of oxidative phosphorylation, the mitochondrial membrane potential, and their role in human disease.
2008,
Pubmed
Jones,
Effects of "priming" exercise on pulmonary O2 uptake and muscle deoxygenation kinetics during heavy-intensity cycle exercise in the supine and upright positions.
2006,
Pubmed
Jouaville,
Regulation of mitochondrial ATP synthesis by calcium: evidence for a long-term metabolic priming.
1999,
Pubmed
Kemp,
Implications of rapid early oxygen consumption in exercising skeletal muscle.
2011,
Pubmed
Korzeniewski,
Regulation of oxidative phosphorylation through parallel activation.
2007,
Pubmed
Korzeniewski,
Biochemical background of the VO2 on-kinetics in skeletal muscles.
2006,
Pubmed
Kuznetsov,
Striking differences between the kinetics of regulation of respiration by ADP in slow-twitch and fast-twitch muscles in vivo.
1996,
Pubmed
Lännergren,
Changes in mitochondrial Ca2+ detected with Rhod-2 in single frog and mouse skeletal muscle fibres during and after repeated tetanic contractions.
2001,
Pubmed
,
Xenbase
Macdonald,
Acceleration of VO2 kinetics in heavy submaximal exercise by hyperoxia and prior high-intensity exercise.
1997,
Pubmed
Mayevsky,
Oxidation-reduction states of NADH in vivo: from animals to clinical use.
2007,
Pubmed
Mayevsky,
Mitochondrial function in vivo evaluated by NADH fluorescence: from animal models to human studies.
2007,
Pubmed
Nicholls,
Interactions between mitochondrial bioenergetics and cytoplasmic calcium in cultured cerebellar granule cells.
2003,
Pubmed
Nicholls,
Forty years of Mitchell's proton circuit: From little grey books to little grey cells.
2008,
Pubmed
Perry,
Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal muscle.
2011,
Pubmed
Phillips,
Intrinsic protein kinase activity in mitochondrial oxidative phosphorylation complexes.
2011,
Pubmed
Picard,
Mitochondrial structure and function are disrupted by standard isolation methods.
2011,
Pubmed
Ramzan,
Mitochondrial respiration and membrane potential are regulated by the allosteric ATP-inhibition of cytochrome c oxidase.
2010,
Pubmed
Richardson,
Evidence of O2 supply-dependent VO2 max in the exercise-trained human quadriceps.
1999,
Pubmed
Rossignol,
Tissue variation in the control of oxidative phosphorylation: implication for mitochondrial diseases.
2000,
Pubmed
Rumsey,
Cellular energetics and the oxygen dependence of respiration in cardiac myocytes isolated from adult rat.
1990,
Pubmed
Scheibye-Knudsen,
Regulation of mitochondrial respiration by inorganic phosphate; comparing permeabilized muscle fibers and isolated mitochondria prepared from type-1 and type-2 rat skeletal muscle.
2009,
Pubmed
Sheldon,
Phosphorylation of voltage-dependent anion channel by serine/threonine kinases governs its interaction with tubulin.
2011,
Pubmed
Stary,
Resistance to fatigue of individual Xenopus single skeletal muscle fibres is correlated with mitochondrial volume density.
2004,
Pubmed
,
Xenbase
Stary,
Effect of varied extracellular PO2 on muscle performance in Xenopus single skeletal muscle fibers.
1999,
Pubmed
,
Xenbase
Timmons,
Substrate availability limits human skeletal muscle oxidative ATP regeneration at the onset of ischemic exercise.
1998,
Pubmed
van der Laarse,
Krogh's diffusion coefficient for oxygen in isolated Xenopus skeletal muscle fibers and rat myocardial trabeculae at maximum rates of oxygen consumption.
2005,
Pubmed
,
Xenbase
Walsh,
Glycolytic activation at the onset of contractions in isolated Xenopus laevis single myofibres.
2008,
Pubmed
,
Xenbase
Ward,
Quantitative analysis of membrane potentials.
2010,
Pubmed
Ward,
Mitochondrial membrane potential and glutamate excitotoxicity in cultured cerebellar granule cells.
2000,
Pubmed
Wüst,
Kinetic control of oxygen consumption during contractions in self-perfused skeletal muscle.
2011,
Pubmed
Zoladz,
Progressive recruitment of muscle fibers is not necessary for the slow component of VO2 kinetics.
2008,
Pubmed
