Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Real-time imaging coupled with a permeabilized cell system presents a very versatile platform to visualize the dynamic and intricate nature of nuclear envelope breakdown, one of the major morphological changes of mitosis. Here, we describe such a strategy in which the plasma membrane of cells expressing fluorescently tagged nucleoporin POM121 and Histone H2B is permeabilized with digitonin. These cells are then incubated with mitotic Xenopus egg extract to create conditions that recapitulate the major events of mitotic nuclear remodeling seen in live-cell imaging, providing the opportunity to probe mechanisms and pathways that coordinate nuclear disassembly.
Beaudouin,
Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina.
2002, Pubmed
Beaudouin,
Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina.
2002,
Pubmed
Blow,
A role for the nuclear envelope in controlling DNA replication within the cell cycle.
1988,
Pubmed
,
Xenbase
Cotter,
Nuclear membrane disassembly and rupture.
2007,
Pubmed
,
Xenbase
Dultz,
Systematic kinetic analysis of mitotic dis- and reassembly of the nuclear pore in living cells.
2008,
Pubmed
Georgatos,
Nuclear envelope breakdown in mammalian cells involves stepwise lamina disassembly and microtubule-drive deformation of the nuclear membrane.
1997,
Pubmed
Güttinger,
Orchestrating nuclear envelope disassembly and reassembly during mitosis.
2009,
Pubmed
Hetzer,
Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly.
2001,
Pubmed
,
Xenbase
Higa,
Studying nuclear disassembly in vitro using Xenopus egg extract.
2006,
Pubmed
,
Xenbase
Kihlmark,
Sequential degradation of proteins from the nuclear envelope during apoptosis.
2001,
Pubmed
Lénárt,
Monitoring the permeability of the nuclear envelope during the cell cycle.
2006,
Pubmed
Lénárt,
Nuclear envelope breakdown in starfish oocytes proceeds by partial NPC disassembly followed by a rapidly spreading fenestration of nuclear membranes.
2003,
Pubmed
Liu,
The COPI complex functions in nuclear envelope breakdown and is recruited by the nucleoporin Nup153.
2003,
Pubmed
,
Xenbase
Mackay,
Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint.
2010,
Pubmed
Mühlhäusser,
An in vitro nuclear disassembly system reveals a role for the RanGTPase system and microtubule-dependent steps in nuclear envelope breakdown.
2007,
Pubmed
,
Xenbase
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Newmeyer,
Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation.
1988,
Pubmed
,
Xenbase
Polychronidou,
Farnesylated nuclear proteins Kugelkern and lamin Dm0 affect nuclear morphology by directly interacting with the nuclear membrane.
2010,
Pubmed
Prunuske,
The nuclear envelope: form and reformation.
2006,
Pubmed
Salina,
Cytoplasmic dynein as a facilitator of nuclear envelope breakdown.
2002,
Pubmed
Volkova,
Self-organization of cellular structures induced by the overexpression of nuclear envelope proteins: a correlative light and electron microscopy study.
2011,
Pubmed
