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Proteins of the AAA (ATPases associated with various cellular activities) family often have complex modes of regulation due to their central position in important cellular processes. p60 katanin, an AAA protein that severs and depolymerizes microtubules, is subject to multiple modes of regulation including a phosphorylation in the N-terminal domain involved in mitotic control of severing. Phosphorylation decreases severing activity in Xenopus egg extracts and is involved in controlling spindle length. Here, we show that the evolutionarily divergent N-terminal domains of p60 have maintained hotspots of mitotic kinase regulation. By reconstituting in vitro severing reactions, we show that phosphomimetic modification at amino acid position 131 in Xenopus laevis p60 decreases severing and microtubule-stimulated ATPase activity without affecting the binding affinity of p60 for microtubules. At high concentrations of the phosphomimetic mutant p60, wild-type levels of activity could be observed, indicating a more switch-like threshold of activity that is controlled by regulating oligomerization on the microtubule. This provides a cellular mechanism for high local concentrations of p60, like those found on spindle poles, to maintain severing activity while most of the protein is inhibited. Overall, we have shown that the modular domain architecture of AAA proteins allows for precise control of cellular activities with simple modifications.
Ahmad,
An essential role for katanin in severing microtubules in the neuron.
1999, Pubmed
Ahmad,
An essential role for katanin in severing microtubules in the neuron.
1999,
Pubmed
Díaz-Valencia,
Drosophila katanin-60 depolymerizes and severs at microtubule defects.
2011,
Pubmed
Dougan,
AAA+ proteins and substrate recognition, it all depends on their partner in crime.
2002,
Pubmed
Dymek,
PF19 encodes the p60 catalytic subunit of katanin and is required for assembly of the flagellar central apparatus in Chlamydomonas.
2012,
Pubmed
Dymek,
PF15p is the chlamydomonas homologue of the Katanin p80 subunit and is required for assembly of flagellar central microtubules.
2004,
Pubmed
Eckert,
Subunit Interactions and cooperativity in the microtubule-severing AAA ATPase spastin.
2012,
Pubmed
Erzberger,
Evolutionary relationships and structural mechanisms of AAA+ proteins.
2006,
Pubmed
Hanson,
AAA+ proteins: have engine, will work.
2005,
Pubmed
Hartman,
Microtubule disassembly by ATP-dependent oligomerization of the AAA enzyme katanin.
1999,
Pubmed
Huang,
Drosophila kinesin minimal motor domain expressed in Escherichia coli. Purification and kinetic characterization.
1994,
Pubmed
Iwaya,
A common substrate recognition mode conserved between katanin p60 and VPS4 governs microtubule severing and membrane skeleton reorganization.
2010,
Pubmed
Lohret,
A role for katanin-mediated axonemal severing during Chlamydomonas deflagellation.
1998,
Pubmed
Loughlin,
Katanin contributes to interspecies spindle length scaling in Xenopus.
2011,
Pubmed
,
Xenbase
Maisel,
The CoxD protein, a novel AAA+ ATPase involved in metal cluster assembly: hydrolysis of nucleotide-triphosphates and oligomerization.
2012,
Pubmed
McNally,
Identification of katanin, an ATPase that severs and disassembles stable microtubules.
1993,
Pubmed
,
Xenbase
McNally,
Two domains of p80 katanin regulate microtubule severing and spindle pole targeting by p60 katanin.
2000,
Pubmed
Mori-Konya,
p97/valosin-containing protein (VCP) is highly modulated by phosphorylation and acetylation.
2009,
Pubmed
Panteris,
A role for katanin in plant cell division: microtubule organization in dividing root cells of fra2 and lue1Arabidopsis thaliana mutants.
2011,
Pubmed
Rasi,
Katanin knockdown supports a role for microtubule severing in release of basal bodies before mitosis in Chlamydomonas.
2009,
Pubmed
Sharma,
Katanin regulates dynamics of microtubules and biogenesis of motile cilia.
2007,
Pubmed
Stoppin-Mellet,
Plant katanin, a microtubule severing protein.
2003,
Pubmed
Uyttewaal,
Mechanical stress acts via katanin to amplify differences in growth rate between adjacent cells in Arabidopsis.
2012,
Pubmed
White,
AAA+ ATPases: achieving diversity of function with conserved machinery.
2007,
Pubmed
Xue,
GPS 2.0, a tool to predict kinase-specific phosphorylation sites in hierarchy.
2008,
Pubmed
Yang,
MEI-1/katanin is required for translocation of the meiosis I spindle to the oocyte cortex in C elegans.
2003,
Pubmed
Ye,
The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol.
2001,
Pubmed
Ye,
Function of the p97-Ufd1-Npl4 complex in retrotranslocation from the ER to the cytosol: dual recognition of nonubiquitinated polypeptide segments and polyubiquitin chains.
2003,
Pubmed
