Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Solid-state NMR investigation of the membrane-disrupting mechanism of antimicrobial peptides MSI-78 and MSI-594 derived from magainin 2 and melittin.
Ramamoorthy A
,
Thennarasu S
,
Lee DK
,
Tan A
,
Maloy L
.
???displayArticle.abstract???
The mechanism of membrane interaction of two amphipathic antimicrobial peptides, MSI-78 and MSI-594, derived from magainin-2 and melittin, is presented. Both the peptides show excellent antimicrobial activity. The 8-anilinonaphthalene-1-sulfonic acid uptake experiment using Escherichia coli cells suggests that the outer membrane permeabilization is mainly due to electrostatic interactions. The interaction of MSI-78 and MSI-594 with lipid membranes was studied using 31P and 2H solid-state NMR, circular dichroism, and differential scanning calorimetry techniques. The binding of MSI-78 and MSI-594 to the lipid membrane is associated with a random coil to alpha-helix structural transition. MSI-78 and MSI-594 also induce the release of entrapped dye from POPC/POPG (3:1) vesicles. Measurement of the phase-transition temperature of peptide-DiPoPE dispersions shows that both MSI-78 and MSI-594 repress the lamellar-to-inverted hexagonal phase transition by inducing positive curvature strain. 15N NMR data suggest that both the peptides are oriented nearly perpendicular to the bilayer normal, which infers that the peptides most likely do not function via a barrel-stave mechanism of membrane-disruption. Data obtained from 31P NMR measurements using peptide-incorporated POPC and POPG oriented lamellar bilayers show a disorder in the orientation of lipids up to a peptide/lipid ratio of 1:20, and the formation of nonbilayer structures at peptide/lipid ratio>1:8. 2H-NMR experiments with selectively deuterated lipids reveal peptide-induced disorder in the methylene units of the lipid acyl chains. These results are discussed in light of lipid-peptide interactions leading to the disruption of membrane via either a carpet or a toroidal-type mechanism.
Beschiaschvili,
Melittin binding to mixed phosphatidylglycerol/phosphatidylcholine membranes.
1990, Pubmed
Beschiaschvili,
Melittin binding to mixed phosphatidylglycerol/phosphatidylcholine membranes.
1990,
Pubmed
Bessalle,
All-D-magainin: chirality, antimicrobial activity and proteolytic resistance.
1990,
Pubmed
,
Xenbase
Boman,
Peptide antibiotics and their role in innate immunity.
1995,
Pubmed
Cornut,
The amphipathic alpha-helix concept. Application to the de novo design of ideally amphipathic Leu, Lys peptides with hemolytic activity higher than that of melittin.
1994,
Pubmed
Dathe,
Peptide helicity and membrane surface charge modulate the balance of electrostatic and hydrophobic interactions with lipid bilayers and biological membranes.
1996,
Pubmed
Dempsey,
The actions of melittin on membranes.
1990,
Pubmed
Eisenberg,
Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
1984,
Pubmed
Epand,
Antimicrobial 14-helical beta-peptides: potent bilayer disrupting agents.
2004,
Pubmed
Glaser,
Concentration-dependent realignment of the antimicrobial peptide PGLa in lipid membranes observed by solid-state 19F-NMR.
2005,
Pubmed
Hallock,
MSI-78, an analogue of the magainin antimicrobial peptides, disrupts lipid bilayer structure via positive curvature strain.
2003,
Pubmed
,
Xenbase
Hallock,
Membrane composition determines pardaxin's mechanism of lipid bilayer disruption.
2002,
Pubmed
Hallock,
An innovative procedure using a sublimable solid to align lipid bilayers for solid-state NMR studies.
2002,
Pubmed
Hancock,
The bacterial outer membrane as a drug barrier.
1997,
Pubmed
Hancock,
Concerns regarding resistance to self-proteins.
2003,
Pubmed
Hawkey,
The origins and molecular basis of antibiotic resistance.
1998,
Pubmed
Henzler Wildman,
Mechanism of lipid bilayer disruption by the human antimicrobial peptide, LL-37.
2003,
Pubmed
Henzler-Wildman,
Perturbation of the hydrophobic core of lipid bilayers by the human antimicrobial peptide LL-37.
2004,
Pubmed
Ladokhin,
'Detergent-like' permeabilization of anionic lipid vesicles by melittin.
2001,
Pubmed
Ladokhin,
Sizing membrane pores in lipid vesicles by leakage of co-encapsulated markers: pore formation by melittin.
1997,
Pubmed
Lu,
Solid-state nuclear magnetic resonance relaxation studies of the interaction mechanism of antimicrobial peptides with phospholipid bilayer membranes.
2005,
Pubmed
Maloy,
Structure-activity studies on magainins and other host defense peptides.
1995,
Pubmed
Marassi,
A solid-state NMR index of helical membrane protein structure and topology.
2000,
Pubmed
,
Xenbase
Marassi,
Complete resolution of the solid-state NMR spectrum of a uniformly 15N-labeled membrane protein in phospholipid bilayers.
1997,
Pubmed
Matsuzaki,
Relationship of membrane curvature to the formation of pores by magainin 2.
1998,
Pubmed
,
Xenbase
Matsuzaki,
Molecular basis for membrane selectivity of an antimicrobial peptide, magainin 2.
1995,
Pubmed
,
Xenbase
Matsuzaki,
Magainins as paradigm for the mode of action of pore forming polypeptides.
1998,
Pubmed
,
Xenbase
Matsuzaki,
An antimicrobial peptide, magainin 2, induced rapid flip-flop of phospholipids coupled with pore formation and peptide translocation.
1996,
Pubmed
,
Xenbase
Mecke,
Membrane thinning due to antimicrobial peptide binding: an atomic force microscopy study of MSI-78 in lipid bilayers.
2005,
Pubmed
Porcelli,
Structure and orientation of pardaxin determined by NMR experiments in model membranes.
2004,
Pubmed
Ramamoorthy,
Experimental aspects of multidimensional solid-state NMR correlation spectroscopy.
1999,
Pubmed
Ramamoorthy,
Three-dimensional solid-state NMR spectroscopy of a peptide oriented in membrane bilayers.
1995,
Pubmed
Rohl,
Comparison of NH exchange and circular dichroism as techniques for measuring the parameters of the helix-coil transition in peptides.
1997,
Pubmed
Seelig,
Investigation of phosphatidylethanolamine bilayers by deuterium and phosphorus-31 nuclear magnetic resonance.
1976,
Pubmed
Shai,
From innate immunity to de-novo designed antimicrobial peptides.
2002,
Pubmed
Shai,
Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by alpha-helical antimicrobial and cell non-selective membrane-lytic peptides.
1999,
Pubmed
,
Xenbase
Sternin,
Acyl chain orientational order in the hexagonal HII phase of phospholipid-water dispersions.
1988,
Pubmed
Thennarasu,
Antimicrobial activity and membrane selective interactions of a synthetic lipopeptide MSI-843.
2005,
Pubmed
Toke,
Secondary structure and lipid contact of a peptide antibiotic in phospholipid bilayers by REDOR.
2004,
Pubmed
Uematsu,
Polar angle as a determinant of amphipathic alpha-helix-lipid interactions: a model peptide study.
2000,
Pubmed
Wade,
All-D amino acid-containing channel-forming antibiotic peptides.
1990,
Pubmed
,
Xenbase
Wang,
Imaging membrane protein helical wheels.
2000,
Pubmed
Wieprecht,
Influence of the angle subtended by the positively charged helix face on the membrane activity of amphipathic, antibacterial peptides.
1997,
Pubmed
Wieprecht,
Modulation of membrane activity of amphipathic, antibacterial peptides by slight modifications of the hydrophobic moment.
1997,
Pubmed
,
Xenbase
Wu,
Mechanism of interaction of different classes of cationic antimicrobial peptides with planar bilayers and with the cytoplasmic membrane of Escherichia coli.
1999,
Pubmed
Zasloff,
Antimicrobial activity of synthetic magainin peptides and several analogues.
1988,
Pubmed
,
Xenbase
Zasloff,
Antimicrobial peptides of multicellular organisms.
2002,
Pubmed
