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Past studies have documented a crosstalk between H2B ubiquitylation (H2Bub) and H3K4 methylation, but little (if any) direct evidence exists explaining the mechanism underlying H2Bub-dependent H3K4 methylation on chromatin templates. Here, we took advantage of an in vitro histone methyltransferase assay employing a reconstituted yeast Set1 complex (ySet1C) and a recombinant chromatin template containing fully ubiquitylated H2B to gain valuable insights. Combined with genetic analyses, we demonstrate that the n-SET domain within Set1, but not Swd2, is essential for H2Bub-dependent H3K4 methylation. Spp1, a homolog of human CFP1, is conditionally involved in this crosstalk. Our findings extend to the human Set1 complex, underscoring the conserved nature of this disease-relevant crosstalk pathway. As not all members of the H3K4 methyltransferase family contain n-SET domains, our studies draw attention to the n-SET domain as a predictor of an H2B ubiquitylation-sensing mechanism that leads to downstream H3K4 methylation.
Briggs,
Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae.
2001, Pubmed
Briggs,
Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae.
2001,
Pubmed
Chandrasekharan,
Histone H2B C-terminal helix mediates trans-histone H3K4 methylation independent of H2B ubiquitination.
2010,
Pubmed
Cheng,
The essential WD repeat protein Swd2 has dual functions in RNA polymerase II transcription termination and lysine 4 methylation of histone H3.
2004,
Pubmed
Chi,
Covalent histone modifications--miswritten, misinterpreted and mis-erased in human cancers.
2010,
Pubmed
Cosgrove,
Mixed lineage leukemia: a structure-function perspective of the MLL1 protein.
2010,
Pubmed
Dehé,
The multiple faces of Set1.
2006,
Pubmed
Dehé,
Histone H3 lysine 4 mono-methylation does not require ubiquitination of histone H2B.
2005,
Pubmed
Dehé,
Protein interactions within the Set1 complex and their roles in the regulation of histone 3 lysine 4 methylation.
2006,
Pubmed
Dichtl,
Functions for S. cerevisiae Swd2p in 3' end formation of specific mRNAs and snoRNAs and global histone 3 lysine 4 methylation.
2004,
Pubmed
Dover,
Methylation of histone H3 by COMPASS requires ubiquitination of histone H2B by Rad6.
2002,
Pubmed
Eissenberg,
Histone H3 lysine 4 (H3K4) methylation in development and differentiation.
2010,
Pubmed
Fierz,
Histone H2B ubiquitylation disrupts local and higher-order chromatin compaction.
2011,
Pubmed
Fingerman,
Global loss of Set1-mediated H3 Lys4 trimethylation is associated with silencing defects in Saccharomyces cerevisiae.
2005,
Pubmed
Halbach,
Cotranslational assembly of the yeast SET1C histone methyltransferase complex.
2009,
Pubmed
Kim,
RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells.
2009,
Pubmed
Lee,
Histone crosstalk between H2B monoubiquitination and H3 methylation mediated by COMPASS.
2007,
Pubmed
McGinty,
Structure-activity analysis of semisynthetic nucleosomes: mechanistic insights into the stimulation of Dot1L by ubiquitylated histone H2B.
2009,
Pubmed
,
Xenbase
McGinty,
Chemically ubiquitylated histone H2B stimulates hDot1L-mediated intranucleosomal methylation.
2008,
Pubmed
,
Xenbase
Nagy,
A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3.
2002,
Pubmed
Nedea,
The Glc7 phosphatase subunit of the cleavage and polyadenylation factor is essential for transcription termination on snoRNA genes.
2008,
Pubmed
Ng,
Targeted recruitment of Set1 histone methylase by elongating Pol II provides a localized mark and memory of recent transcriptional activity.
2003,
Pubmed
Patel,
A conserved arginine-containing motif crucial for the assembly and enzymatic activity of the mixed lineage leukemia protein-1 core complex.
2008,
Pubmed
Racine,
Histone H2B ubiquitylation promotes activity of the intact Set1 histone methyltransferase complex in fission yeast.
2012,
Pubmed
,
Xenbase
Roguev,
High conservation of the Set1/Rad6 axis of histone 3 lysine 4 methylation in budding and fission yeasts.
2003,
Pubmed
Roguev,
The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4.
2001,
Pubmed
Ruthenburg,
Methylation of lysine 4 on histone H3: intricacy of writing and reading a single epigenetic mark.
2007,
Pubmed
Santos-Rosa,
Active genes are tri-methylated at K4 of histone H3.
2002,
Pubmed
Schlichter,
Histone trimethylation by Set1 is coordinated by the RRM, autoinhibitory, and catalytic domains.
2005,
Pubmed
Schübeler,
The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote.
2004,
Pubmed
Shahbazian,
Histone H2B ubiquitylation controls processive methylation but not monomethylation by Dot1 and Set1.
2005,
Pubmed
Soares,
Histone Crosstalk: H2Bub and H3K4 Methylation.
2013,
Pubmed
Soares,
Yeast Swd2 is essential because of antagonism between Set1 histone methyltransferase complex and APT (associated with Pta1) termination factor.
2012,
Pubmed
Song,
WDR5 interacts with mixed lineage leukemia (MLL) protein via the histone H3-binding pocket.
2008,
Pubmed
South,
A conserved interaction between the SDI domain of Bre2 and the Dpy-30 domain of Sdc1 is required for histone methylation and gene expression.
2010,
Pubmed
Sun,
Ubiquitination of histone H2B regulates H3 methylation and gene silencing in yeast.
2002,
Pubmed
Vitaliano-Prunier,
Ubiquitylation of the COMPASS component Swd2 links H2B ubiquitylation to H3K4 trimethylation.
2008,
Pubmed
Wang,
Histone H2B ubiquitylation is not required for histone H3 methylation at lysine 4 in tetrahymena.
2009,
Pubmed
Wang,
"Misinterpretation" of a histone mark is linked to aberrant stem cells and cancer development.
2009,
Pubmed
