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ScientificWorldJournal
2012 Jan 01;2012:369802. doi: 10.1100/2012/369802.
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Assessment of tools for marker-assisted selection in a marine commercial species: significant association between MSTN-1 gene polymorphism and growth traits.
Sánchez-Ramos I
,
Cross I
,
Mácha J
,
Martínez-Rodríguez G
,
Krylov V
,
Rebordinos L
.
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Growth is a priority trait from the point of view of genetic improvement. Molecular markers linked to quantitative trait loci (QTL) have been regarded as useful for marker-assisted selection in complex traits as growth. Polymorphisms have been studied in five candidate genes influencing growth in gilthead seabream (Sparus aurata): the growth hormone (GH), insulin-like growth factor-1 (IGF-1), myostatin (MSTN-1), prolactin (PRL), and somatolactin (SL) genes. Specimens evaluated were from a commercial broodstock comprising 131 breeders (from which 36 males and 44 females contributed to the progeny). In all samples eleven gene fragments, covering more than 13,000 bp, generated by PCR-RFLP, were analyzed; tests were made for significant associations between these markers and growth traits. ANOVA results showed a significant association between MSTN-1 gene polymorphism and growth traits. Pairwise tests revealed several RFLPs in the MSTN-1 gene with significant heterogeneity of genotypes among size groups. PRL and MSTN-1 genes presented linkage disequilibrium. The MSTN-1 gene was mapped in the centromeric region of a medium-size acrocentric chromosome pair.
Figure 1. Schematic diagram of sampling during the different life stages.
Figure 2. Structure of the candidate genes and oligonucleotide primers used for amplification by PCR in this study. Growth hormone (a), Insulin-Like Growth Factor I (b), Myostatin-1 (c), Prolactin (d), and Somatolactin (e) genes. I: Introns; E: Exons; Arrows indicate relative position of the primers.
Figure 3. Localization of the myostatin-1 probe on S. aurata chromosomes. (a) MSTN-1 probe in the centromeric region of acrocentric chromosomes. (b) Localization of MSTN-1 and putative MSTN-2 genes both in centromeric regions of two different acrocentric chromosomes.
Almuly,
Genomic structure and sequence of the gilthead seabream (Sparus aurata) growth hormone-encoding gene: identification of minisatellite polymorphism in intron I.
2000, Pubmed
Almuly,
Genomic structure and sequence of the gilthead seabream (Sparus aurata) growth hormone-encoding gene: identification of minisatellite polymorphism in intron I.
2000,
Pubmed
Bikandi,
In silico analysis of complete bacterial genomes: PCR, AFLP-PCR and endonuclease restriction.
2004,
Pubmed
Cross,
Cytogenetic characterization of the sole Solea senegalensis (Teleostei: Pleuronectiformes: Soleidae): Ag-NOR, (GATA)n, (TTAGGG)n and ribosomal genes by one-color and two-color FISH.
2006,
Pubmed
Goudet,
Testing differentiation in diploid populations.
1996,
Pubmed
Grobet,
A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle.
1997,
Pubmed
Krylov,
Localization of the single copy gene Mdh2 on Xenopus tropicalis chromosomes by FISH-TSA.
2007,
Pubmed
,
Xenbase
Maccatrozzo,
Characterization of the myostatin gene in the gilthead seabream (Sparus aurata): sequence, genomic structure, and expression pattern.
2001,
Pubmed
Maccatrozzo,
A novel second myostatin gene is present in teleost fish.
2001,
Pubmed
Manzon,
The role of prolactin in fish osmoregulation: a review.
2002,
Pubmed
Martínez,
Protein salting-out method applied to genomic DNA isolation from fish whole blood.
1998,
Pubmed
McPherron,
Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member.
1997,
Pubmed
Ng,
A physical map of the genome of Atlantic salmon, Salmo salar.
2005,
Pubmed
Phillips,
Application of fluorescence in situ hybridization (FISH) to fish genetics and genome mapping.
2001,
Pubmed
Prudence,
An amylase gene polymorphism is associated with growth differences in the Pacific cupped oyster Crassostrea gigas.
2006,
Pubmed
Radaelli,
Myostatin precursor is present in several tissues in teleost fish: a comparative immunolocalization study.
2003,
Pubmed
Rice,
ANALYZING TABLES OF STATISTICAL TESTS.
1989,
Pubmed
Rozen,
Primer3 on the WWW for general users and for biologist programmers.
2000,
Pubmed
Sarropoulou,
A gene-based radiation hybrid map of the gilthead sea bream Sparus aurata refines and exploits conserved synteny with Tetraodon nigroviridis.
2007,
Pubmed
Tang,
Myostatin DNA vaccine increases skeletal muscle mass and endurance in mice.
2007,
Pubmed
Wang,
SNPs in the myostatin gene of the mollusk Chlamys farreri: association with growth traits.
2010,
Pubmed
Wang,
Polymorphism of heart fatty acid-binding protein gene associatied with fatness traits in the chicken.
2007,
Pubmed
Welle,
Relation between extent of myostatin depletion and muscle growth in mature mice.
2009,
Pubmed
Xu,
Analysis of myostatin gene structure, expression and function in zebrafish.
2003,
Pubmed
Zhang,
Associations of a HinfI PCR-RFLP of POU1F1 gene with growth traits in Qinchuan cattle.
2009,
Pubmed
