Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Rhodopsin is trafficked to the rod outer segment of vertebrate rod cells with high fidelity. When rhodopsin transport is disrupted retinal photoreceptors apoptose, resulting in the blinding disease autosomal dominant retinitis pigmentosa. Herein, we introduce rhodopsin-photoactivatable GFP-1D4 (rhodopsin-paGFP-1D4) for the purposes of monitoring rhodopsin transport in living cells. Rhodopsin-paGFP-1D4 contains photoactivatable GFP (paGFP) fused to rhodopsin's C-terminus and the last eight amino acids of rhodopsin (1D4) appended to the C-terminus of paGFP. The fusion protein binds the chromophore 11-cis retinal and photoisomerizes upon light activation similarly to rhodopsin. It activates the G-protein transducin with similar kinetics as does rhodopsin. Rhodopsin-paGFP-1D4 localizes to the same compartments, the primary cilium in cultured IMCD cells and the outer segment of rod cells, as rhodopsin in vitro and in vivo. This enables its use as a model of rhodopsin transport and details the importance of a free rhodopsin C-terminus in rod cell localization and health.
???displayArticle.pubmedLink???
24140958
???displayArticle.pmcLink???PMC3848712 ???displayArticle.link???Vision Res ???displayArticle.grants???[+]
Chuang,
The cytoplasmic tail of rhodopsin acts as a novel apical sorting signal in polarized MDCK cells.
1998, Pubmed
Chuang,
The cytoplasmic tail of rhodopsin acts as a novel apical sorting signal in polarized MDCK cells.
1998,
Pubmed
Concepcion,
The carboxyl-terminal domain is essential for rhodopsin transport in rod photoreceptors.
2002,
Pubmed
Dryja,
Mutations within the rhodopsin gene in patients with autosomal dominant retinitis pigmentosa.
1990,
Pubmed
Dryja,
Mutation spectrum of the rhodopsin gene among patients with autosomal dominant retinitis pigmentosa.
1991,
Pubmed
Gross,
Characterization of rhodopsin congenital night blindness mutant T94I.
2003,
Pubmed
Gross,
Defective development of photoreceptor membranes in a mouse model of recessive retinal degeneration.
2006,
Pubmed
Janz,
Rhodopsin activation exposes a key hydrophobic binding site for the transducin alpha-subunit C terminus.
2004,
Pubmed
Jin,
An improved rhodopsin/EGFP fusion protein for use in the generation of transgenic Xenopus laevis.
2003,
Pubmed
,
Xenbase
Li,
Transgenic mice carrying the dominant rhodopsin mutation P347S: evidence for defective vectorial transport of rhodopsin to the outer segments.
1996,
Pubmed
Lodowski,
Signals governing the trafficking and mistrafficking of a ciliary GPCR, rhodopsin.
2013,
Pubmed
,
Xenbase
Millar,
The year in G protein-coupled receptor research.
2010,
Pubmed
Ogino,
High-throughput transgenesis in Xenopus using I-SceI meganuclease.
2006,
Pubmed
,
Xenbase
Oprian,
Expression of a synthetic bovine rhodopsin gene in monkey kidney cells.
1987,
Pubmed
Overington,
How many drug targets are there?
2006,
Pubmed
Palczewski,
Chemistry and biology of vision.
2012,
Pubmed
Patterson,
A photoactivatable GFP for selective photolabeling of proteins and cells.
2002,
Pubmed
Pédelacq,
Engineering and characterization of a superfolder green fluorescent protein.
2006,
Pubmed
Rauchman,
An osmotically tolerant inner medullary collecting duct cell line from an SV40 transgenic mouse.
1993,
Pubmed
Ruta,
A dimorphic pheromone circuit in Drosophila from sensory input to descending output.
2010,
Pubmed
Saga,
Development and maintenance of outer segments by isolated chick embryo photoreceptor cells in culture.
1996,
Pubmed
Sammons,
In vitro biochemical assays to monitor rhodopsin function.
2012,
Pubmed
Stenkamp,
Photoreceptor differentiation of isolated retinal precursor cells includes the capacity for photomechanical responses.
1993,
Pubmed
Sung,
A rhodopsin gene mutation responsible for autosomal dominant retinitis pigmentosa results in a protein that is defective in localization to the photoreceptor outer segment.
1994,
Pubmed
Tam,
Mislocalized rhodopsin does not require activation to cause retinal degeneration and neurite outgrowth in Xenopus laevis.
2006,
Pubmed
,
Xenbase
Tam,
Identification of an outer segment targeting signal in the COOH terminus of rhodopsin using transgenic Xenopus laevis.
2000,
Pubmed
,
Xenbase
Trivedi,
Live-cell imaging evidence for the ciliary transport of rod photoreceptor opsin by heterotrimeric kinesin-2.
2012,
Pubmed
Wensel,
Rhodopsin-EGFP knock-ins for imaging quantal gene alterations.
2005,
Pubmed
Zeitz,
Identification and functional characterization of a novel rhodopsin mutation associated with autosomal dominant CSNB.
2008,
Pubmed
Zvyaga,
Characterization of the mutant visual pigment responsible for congenital night blindness: a biochemical and Fourier-transform infrared spectroscopy study.
1996,
Pubmed
