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During Xenopus laevis neurulation, neural ectodermal cells of the spinal cord are patterned at the same time that they intercalate mediolaterally and radially, moving within and between two cell layers. Curious if these rearrangements disrupt early cell identities, we lineage-traced cells in each layer from neural plate stages to the closed neural tube, and used in situ hybridization to assay gene expression in the moving cells. Our biotin and fluorescent labeling of deep and superficial cells reveals that mediolateral intercalation does not disrupt cell cohorts; in other words, it is conservative. However, outside the midline notoplate, later radial intercalation does displace superficial cells dorsoventrally, radically disrupting cell cohorts. The tube roof is composed almost exclusively of superficial cells, including some displaced from ventral positions; gene expression in these displaced cells must now be surveyed further. Superficial cells also flank the tube's floor, which is, itself, almost exclusively composed of deep cells. Our data provide: (1) a fate map of superficial- and deep-cell positions within the Xenopus neural tube, (2) the paths taken to these positions, and (3) preliminary evidence of re-patterning in cells carried out of one environment and into another, during neural morphogenesis.
Fig. 6. Representative confocal, transverse optical sections of a neural tube containing a rhodamine-labeled superficial-ectodermal implant and
fluorescent label for Xenopus Pax3 gene expression. Single-layer, superficial cell grafts were placed in the neural plates of 7 hosts at stage 11.5,
and embryos grown to stage 23, before Pax3 gene expression was detected using RNA in situ hybridization (Davidson and Keller, 1999). Fluorescein
visualization reveals Pax3 RNA within rhodamine-containing superficial cells en route to the dorsal neural tube.
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