Falk J , Konopacki FA , Zivraj KH , Holt CE .

Wellcome Trust

	Figure 1. Constitutive recycling exhibited by Xenopus RGC growth cones is necessary for axon elongation in vitro. A–C, Biotinylated cell surface proteins are internalized and recycled in RGC growth cones.A, Biotinylated proteins were allowed to internalize for 20 min, after which the biotin still present at the surface was removed byMESNAand intracellular accumulations of biotinylated protein were detected in permeabilized growth cones. B, As expected, biotin labeling was barely detected just after MESNA treatment in nonpermeabilized growth cones. C, Twenty minutes later, however, some biotin labeling is recovered at the surface of nonpermeabilized growth cones. D–F, Primaquine reduces local recycling. D, FM dye is internalized in vesicles of different sizes that accumulate in the central domain of the growth cone; the white line delineates the outline of the growth cone. E, The endosomes in growth cones were loaded withFM4–64 and time-lapse imaging was performed. The total loss of FM4–64 fluorescence over time represents the speed of local recycling as individual FM dyeloaded vesicles release their content to the environment. Note that primaquine (0.068 mM) reduces local recycling, as more FM dye is retained in primaquine-treated growth cones. The outlines of growth cones are shown in the white broken line. F, The histogram shows the quantification of the total FM4–64 fluorescence intensity in the growth cones over time. Note that the difference in recycling between control and primaquine-treated growth cones becomes significant after 2 min. G,H, Primaquine blocks axon extension in vitro. G, The histogram shows the quantification of the extension rate of axons before, during, and after primaquine treatment. Numbers above bars indicate the number of axons analyzed.H, Snapshots from an in vitro time-lapse sequence illustrate the axon elongation rate of the same GFP-expressing axons in the absence of (left), in the presence of (middle), and after the dilution (right) of primaquine. For each axon, the dash and the arrowhead indicate the growth cone positions at the beginning and the end of the 45 min recording period, respectively. The dotted lines represent the distances covered by the growth cones during that time and exemplify the sharp inhibition of elongation observed in presence of primaquine. Error bars represent the SEM. *p  0.05; ***p0.001. Scale bars: A–C, 5m; D, 4m; E, 5m; H, 30m.
	Figure 2. RGC growth cones contain Rab5-, Rab4-, and Rab11-positive endosomes. A, Rab5 endogenous protein is detected in axon shaft and growth cone of retinal ganglion cells by immunocytochemistry. Rab5 labeling appears as focal puncta suggestive of vesicles in both the central (filled green arrowheads) and peripheral (open red arrowheads) domains of the growth cone. B–E, Xenopus Rab5c fused to GFP labels vesicles in both domains of RGC growth cones in vitro (B–D) and in vivo (E). C, D, Small vesicles are frequently observed in filopodia or lamellipodia. F, RFP-Rab5c labels vesicles that recruit FYVE-GFP (another marker of early endosomes).G, Rab4 is detected in the axon and growth cone of RGCs. Immunoreactivity exhibits vesicular-appearing signals in both domains of the growth cone. H–J, Xenopus GFP-Rab4 accumulates on vesicle-like structures present in both domains of the growth cone. I, J, Rab4 vesicles are found in filopodia and lamellipodia. K, GFP-Rab11b associates with small vesicles present in the axon shaft and both domains of the growth cone. Filled green and open red arrowheads point to vesicles located in the central and peripheral domains respectively. L, Confocal images of growth cone coexpressing RFP-Rab4 and GFP-Rab5c show that small Rab4-positive endosomes in the periphery are distinct from Rab5 ones (arrowheads). Rab4 and Rab5c colocalize, however, on the large vesicles frequently found in the central domain (asterisk). M, Confocal images show that Rab4 endosomes are distinct from the acidic LysoTracker-stained late endosomes and lysosomes. B, F, J, L, and M were acquired with a confocal spinning disk. Scale bars: A–L, 2m;M, 3m.
	Figure 3. FRAP analysis indicates that Rab5- and Rab4-positive endosomes could form locally. A, The schematic illustrates the use of FRAP to define the origin of the Rab-positive endosomes present in the growth cone. The GFP-positive endosomes that reappear after photobleaching can be (1) unbleached endosomes that moved from the axon or (2) endosomes that have recruited unbleached cytosolic Rab5c-GFP that diffused into the growth cone. B–F, Representative images of the GFP signal before bleaching, immediately after, and during the recovery in growth cone expressing GFP-Rab5c (B–D), GFP-Rab4 (E), or GFP-Rab11 (F). B, Rab5c-positive endosomes have reappeared in the bleached growth cone, although many of the axonal Rab5c-positive endosomes remained in the axon (white arrowheads). C, Tracking of the endosomes present in the growth cone suggests that the majority of the Rab5c-positive endosomes does not come from the axons as only one of five endosomes labeled could be traced back to the axon. D, Rab5 signal is recovered on both long-lasting large endosomes and small dynamic endosomes. The small endosomes in the lamellipodia (open arrowhead at18 s) move or disappear within 10 s. In contrast, large endosomes (filled arrowhead) remain stable and show little displacement. During the recovery, the large endosomes progressively recover Rab5 signal (filled arrowhead). In addition, small endosomes appear suddenly in the peripheral domain (open arrowheads at 180 and 190 s). E, Still images illustrating the GFP-Rab4 signal during the FRAP experiment. As with GFP-Rab5, GFP-Rab4 signal is recovered in the growth cone. Similarly,manyRab4-positive endosomes that appear in the growth cone after photobleaching could not be related to axonal endosomes (E). In contrast, most of the Rab11-positive endosomes come from the axon (F). Open arrowheads identify endosomes that appear to have formed within the growth cone. Filled arrowheads point to the endosomes that have moved from the axon. Dotted lines outline the bleached area. " denotes seconds Scale bars: A, C–F, 2m;B, 4m.
	Figure 4. Dynamic analysis of Rab4-positive endosomes suggests that they support local recycling in the growth cone. A, Time-lapse confocal images show a Rab4-positive tubule (arrowhead) that buds and splits from a large Rab5c- and Rab4-positive endosome. The 2 Hz time-lapse imaging was performed on growth cones coexpressing wild-type Xenopus GFP-Rab5c and RFPRab4. B, After photobleaching, the RFP-Rab4 signal recovers on large GFP-Rab5c-positive endosomes in RGC growth cone. Left, Prebleaching signal (4 s). Middle, The RFP signal, but not the GFP signal, is lost after photobleaching (0 s). As illustrated in the left panel (150 s), during recovery, the RFP signal is progressively recovered on the large GFP-positive endosome (red arrowhead). Rab4 is not recruited to Rab5 endosomes that were Rab4-negative (white arrowhead). C, Bleaching of the peripheral domain reveals the shipment of small Rab4-positive endosomes from the central to the peripheral domain (green arrowheads). D, Time-lapse images illustrate the entry of a small Rab4-positive (Rab5-negative) endosome into a filopodium and its movement toward the tip. Arrowheads mark the position of the endosome at different time points. E, F, Confocal images show that internalized biotinylated proteins (red) partially colocalize with vesicles labeled by GFP-Rab4 (green). The small Rab4-positive endosomes present in filopodia also contain biotinylated proteins (arrowhead, F). " denotes seconds. Scale bars, 2um.
	Figure 5. Constitutively active Rab5 slows the axon extension rate in vitro.A, The electroporation of CA-Rab5c in RGCs induces the formation of enlarged endosomes. In the RGC soma, CA-Rab5c that lacksGTPaseactivity(QLmutant)labelsendosomesthatareconsiderablylargerthanthoselabeledby wild-type GFP-Rab5c. B, C, Still images from time-lapse sequences illustrate axon extension of GFPexpressing (B) and constitutively active GFP-Rab5c-expressing (C) axons. Time-lapse imaging was performed on whole eyes from stage 33/34 embryos that had been cultured overnight. Arrowheads indicate the positions of the growth cone at 0 and 45 min. Dotted lines exemplify the distance extended by the growth cones during the 45minrecording period.D, The histogram shows the average extension rate of axons expressing GFP, the Xenopus CA-Rab5c we generated (xRab5QL), and the previously characterized zebrafish CA-Rab5c (zRab5c) constructs. E, Cumulative distributions of the apparent diameter of the endosomes labeled by GFP-CA-Rab5c (black line; N64) and wild-type GFP-Rab5c (gray dotted line; N404) suggest that the CA-Rab5c leads to an enlargement of the Rab5-positive endosomes in the growth cone. Numbers above the bars indicate the number of axons analyzed. Error bars represent the SEM. ***p0.001 vs the control (GFP). ' denotes minutes. Scale bars: A, 2um; B, C, 30um.
	Figure 6. Dominant-negative Rab4 slows the axon extension rate in vitro. A, Primaquine treatment alters Rab4-positive endosomes. Images of Rab4-expressing axons before (left) and 45 min after (right) primaquine application (0.05 mM) illustrate the appearance of large abnormal Rab4-positive tubules (arrowheads). B, An example of RGC growth cone expressing the dominantnegative Xenopus GFP-tagged Rab4-NI mutant. Note that, in contrast to wild type (A, left), the mutant does not display a punctate distribution. C–E, DN-Rab4 construct impairs axon extension. C, D, Images of GFP-positive axons at the start and the end of the time-lapse recording illustrate the extension of GFP-expressing (C) and DN-xRab4-expressing (D) axons. Arrowheads indicate the positions of the growth cones at 0 and 45 min. Dotted lines highlight the distance extended by the growth cones during this period. E, The histograms show the average extension rate measured from time-lapse recording of axons that expressed GFP, DN-xRab4 (NI mutant), or CA-xRab4 (QL mutant). F, Rab7 mutants do not slow axon extension in RGCs. The extension rate of that axon that expressed DN-Rab7 (TN mutation) or CA-Rab7 (QL mutation) was measured from time-lapse recording and compared with the extension rate of GFP-expressing axons from the same electroporation batch. Neither Xenopus (xRab7) nor human (hRab7) DNRab7 decreases the axon extension rate. Numbers above the bars indicate the number of axons (E, F). Error bars represent the SEM. ***p0.001 vs the control. Scale bars: A, B, 5um; C, D, 30um.
	Figure 7. Rab4 knockdown impairs axon elongation in vitro. A, The assessment of the effect of Rab4 morpholino on developing Xenopus embryos. Shown are typical examples of embryos at stage 33–34, injected with 12 ng/blastomere fluorescein-tagged control morpholino (top) or Rab4 morpholino (bottom). Note that, at this dose, the Rab4 morpholino does not cause any gross developmental abnormalities.B, Rab4 morpholino reduces the Rab4 protein levels approximately by half. Shown is a representative example of a Western blot of brain-and-eye protein extracts from stage 37 embryos. C, Rab4 morpholino reduces the levels of Rab4 protein in RGC growth cones of cultured eye explants by 50.16.2% relative to control morpholino. All quantified growth cones contain green fluorescence from the morpholino tag. Actin (phalloidin) staining was used to show the outline of the growth cone. D, Rab4 morpholino impairs axonal outgrowth in vitro. The extension of axons containing either control morpholino (green, top row) or Rab4 morpholino (green, bottom row) was monitored over 45 min. Corresponding phase-contrast images (grayscale) were used for actual measurements. Scale bars: C, 10m; D, 25m. E, Quantification of D. Axon outgrowth is significantly reduced in cells containing Rab4 morpholino. Cumulative distribution plots show that both datasets: control morpholino (red line) and Rab4 morpholino (blue line) are significantly different from each other ( p5.15106, Student’s t test).
	Figure 8. Constitutively active Rab5 delays axon arrival at the tectum in vivo. A, Schematic representation of the experiment; retinas of Xenopus embryos were electroporated when RGCs are generated, and embryos were killed before or after RGC axons have reached the tectum (i.e., stage 37/38 or 39). B, Right, 3D representation of the retino-tectal projection in Xenopus embryo. RGC axons from the contralateral eye (black) enter the brain at the optic chiasm and grow dorsally in the diencephalon before turning caudally to enter their target, the tectum. Left, Phase image of a stage 39 hemi-brain depicts how brain and tectum outlines (dashed lines) are drawn. The normal course and length of the RGC axons is presented (black lines). C–E, RGC axons that express CA-Rab5c are shorter than the control. C, D, Lateral views of whole-mount hemi-brain from stage 37/38 embryos show that GFP-expressing axons approach the tectum (C), while CA-GFP-Rab5-expressing ones are still far away (D). The length of the longest axons corresponding to the dotted lines (C, D) was measured, and the quantifications are presented in E. The experiments were performed with the Xenopus CA-Rab5c (xRab5QL) and the zebrafish CA-Rab5c (zRab5c) constructs, and were analyzed at stage 37/38 and stage 39 in both cases. Numbers above the bars indicate the number of embryos analyzed. Error bars represent the SEM. ***p0.001 vs the control. Scale bars: B, D, 100m. OC, Optic chiasm; Di., diencephalon; Tel., telencephalon; Pin., pineal gland.
	Figure 9. DN-Rab4 delays axon arrival at the tectum and slows axon extension in vivo. A–C, Lateral views of stage 37/38 contralateral brains illustrate the length of GFP-positive (A), DN-GFP-Rab4-positive (B), and CA-GFP-Rab4-positive (C) axons. The tectum (dashed line) and the brain (plain line) outlines were drawn from the phase image. Dotted lines between arrowheads exemplify the length of the longest axon. Axons that express GFP (A) or GFP-CA-Rab4 (QL mutant; C) extend equally well along the optic tract. In contrast, DN-Rab4 (NI mutant)-expressing axons are much shorter (B). Lengths of GFP-positive, CA-Rab4-positive, DN-Rab4-positive, and DN-Rab7-positive (Xenopus, human) axons were measured in stage 37/38 and 39 brains. D–F, Summary of the quantifications shows that DN-Rab4 is the only construct tested here that significantly reduces axon length. G, H, DN-Rab4 decreases axon extension rate in vivo. G, Pictures from in vivo time-lapse sequences show DN-GFP-Rab4-expressing (left) and GFP-expressing (right) axons that extend along the optic tract. Images are z-projections at the 0 and 60 min time points. Open and filled arrowheads indicate the growth cone positions at the start and the end of the 1 h recording, respectively. Dotted lines exemplify the distance traveled by the growth cones during the 1 h monitoring. Quantifications of the in vivo time-lapse recordings are presented inH. Numbers above bars indicate the number of embryos (D–F) or axons (H) analyzed. Error bars represent the SEM. ***p0.001 vs the control. Scale bars: C, 100um; G, 15um.

Archer, Detection call algorithms for high-throughput gene expression microarray data. 2010, Pubmed

Archer, Detection call algorithms for high-throughput gene expression microarray data. 2010, Pubmed
Ascaño, Axonal targeting of Trk receptors via transcytosis regulates sensitivity to neurotrophin responses. 2009, Pubmed
Bananis, Regulation of early endocytic vesicle motility and fission in a reconstituted system. 2003, Pubmed
Barbieri, Epidermal growth factor and membrane trafficking. EGF receptor activation of endocytosis requires Rab5a. 2000, Pubmed
Bergtold, Cell surface recycling of internalized antigen permits dendritic cell priming of B cells. 2005, Pubmed
Bonanomi, Phosphorylation of synapsin I by cAMP-dependent protein kinase controls synaptic vesicle dynamics in developing neurons. 2005, Pubmed
Bonanomi, Identification of a developmentally regulated pathway of membrane retrieval in neuronal growth cones. 2008, Pubmed
Brown, Functional compartmentalization of endosomal trafficking for the synaptic delivery of AMPA receptors during long-term potentiation. 2007, Pubmed
Bu, The Toca-1-N-WASP complex links filopodial formation to endocytosis. 2009, Pubmed
Bucci, Rab7: a key to lysosome biogenesis. 2000, Pubmed
Bucci, Co-operative regulation of endocytosis by three Rab5 isoforms. 1995, Pubmed
Campbell, Semaphorin 3A elicits stage-dependent collapse, turning, and branching in Xenopus retinal growth cones. 2001, Pubmed , Xenbase
Capps, Phosphorylation of class I MHC molecules in the absence of phorbol esters is an intracellular event and may be characteristic of trafficking molecules. 2000, Pubmed
Carcea, Flotillin-mediated endocytic events dictate cell type-specific responses to semaphorin 3A. 2010, Pubmed
Castellani, Semaphorin3A-induced receptor endocytosis during axon guidance responses is mediated by L1 CAM. 2004, Pubmed
Chavrier, Early endosome membrane dynamics characterized by flow cytometry. 1997, Pubmed
Chen, Rab5 isoforms differentially regulate the trafficking and degradation of epidermal growth factor receptors. 2009, Pubmed
Chevallier, Rab35 regulates neurite outgrowth and cell shape. 2009, Pubmed
Chien, Navigational errors made by growth cones without filopodia in the embryonic Xenopus brain. 1993, Pubmed , Xenbase
Cormont, Potential role of Rab4 in the regulation of subcellular localization of Glut4 in adipocytes. 1996, Pubmed
Cowan, Vav family GEFs link activated Ephs to endocytosis and axon guidance. 2005, Pubmed
de Hoop, The involvement of the small GTP-binding protein Rab5a in neuronal endocytosis. 1994, Pubmed
Deinhardt, Rab5 and Rab7 control endocytic sorting along the axonal retrograde transport pathway. 2006, Pubmed
Deneka, Rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 and gamma(1)-adaptin in membrane recycling from endosomes. 2003, Pubmed
de Wit, Rab4 regulates formation of synaptic-like microvesicles from early endosomes in PC12 cells. 2001, Pubmed
Diefenbach, Membrane recycling in the neuronal growth cone revealed by FM1-43 labeling. 1999, Pubmed
Diestel, NCAM is ubiquitylated, endocytosed and recycled in neurons. 2007, Pubmed
Dwivedy, Ena/VASP function in retinal axons is required for terminal arborization but not pathway navigation. 2007, Pubmed , Xenbase
Eva, Rab11 and its effector Rab coupling protein contribute to the trafficking of beta 1 integrins during axon growth in adult dorsal root ganglion neurons and PC12 cells. 2010, Pubmed
Falk, Electroporation of cDNA/Morpholinos to targeted areas of embryonic CNS in Xenopus. 2007, Pubmed , Xenbase
Feliciano, Coordination of the Rab5 cycle on macropinosomes. 2011, Pubmed
Fournier, Semaphorin3A enhances endocytosis at sites of receptor-F-actin colocalization during growth cone collapse. 2000, Pubmed
Glodowski, RAB-10 regulates glutamate receptor recycling in a cholesterol-dependent endocytosis pathway. 2007, Pubmed
Goldberg, How does an axon grow? 2003, Pubmed
Gomez, In vivo regulation of axon extension and pathfinding by growth-cone calcium transients. 1999, Pubmed , Xenbase
Granseth, Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses. 2006, Pubmed
Grant, Pathways and mechanisms of endocytic recycling. 2009, Pubmed
Gu, Integrins traffic rapidly via circular dorsal ruffles and macropinocytosis during stimulated cell migration. 2011, Pubmed
Harris, Retinal axons with and without their somata, growing to and arborizing in the tectum of Xenopus embryos: a time-lapse video study of single fibres in vivo. 1987, Pubmed , Xenbase
Harris, Homing behaviour of axons in the embryonic vertebrate brain. , Pubmed , Xenbase
Hayakawa, Evolutionarily conserved structural and functional roles of the FYVE domain. 2007, Pubmed
Heo, Coordinate Regulation of Neurite Outgrowth by LRRK2 and Its Interactor, Rab5. 2010, Pubmed
Hines, Asymmetric endocytosis and remodeling of beta1-integrin adhesions during growth cone chemorepulsion by MAG. 2010, Pubmed , Xenbase
Hines, Single vesicle imaging indicates distinct modes of rapid membrane retrieval during nerve growth. 2012, Pubmed , Xenbase
Holmes, Differential regulation of corticotropin releasing factor 1alpha receptor endocytosis and trafficking by beta-arrestins and Rab GTPases. 2006, Pubmed
Holt, Order in the initial retinotectal map in Xenopus: a new technique for labelling growing nerve fibres. 1983, Pubmed , Xenbase
Holt, Does timing of axon outgrowth influence initial retinotectal topography in Xenopus? 1984, Pubmed , Xenbase
Hoogenraad, Neuron specific Rab4 effector GRASP-1 coordinates membrane specialization and maturation of recycling endosomes. 2010, Pubmed
Horiuchi, A GDP/GTP exchange-stimulatory activity for the Rab5-RabGDI complex on clathrin-coated vesicles from bovine brain. 1995, Pubmed
Jones, VEGFR1 (Flt1) regulates Rab4 recycling to control fibronectin polymerization and endothelial vessel branching. 2009, Pubmed
Joset, Pincher-generated Nogo-A endosomes mediate growth cone collapse and retrograde signaling. 2010, Pubmed
Jullien, Morphogen gradient interpretation by a regulated trafficking step during ligand-receptor transduction. 2005, Pubmed , Xenbase
Jurney, Rac1-mediated endocytosis during ephrin-A2- and semaphorin 3A-induced growth cone collapse. 2002, Pubmed
Juvet, The expression of endosomal rab proteins correlates with endocytic rate in rat liver cells. 1997, Pubmed
Kabayama, Ca2+ induces macropinocytosis via F-actin depolymerization during growth cone collapse. 2009, Pubmed
Kaddai, Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte. 2009, Pubmed
Kamiguchi, The role of endocytic l1 trafficking in polarized adhesion and migration of nerve growth cones. 2001, Pubmed
Kamiguchi, Recycling of the cell adhesion molecule L1 in axonal growth cones. 2000, Pubmed
Kolpak, Negative guidance factor-induced macropinocytosis in the growth cone plays a critical role in repulsive axon turning. 2009, Pubmed
Kouranti, Rab35 regulates an endocytic recycling pathway essential for the terminal steps of cytokinesis. 2006, Pubmed
Lapierre, Rab11b resides in a vesicular compartment distinct from Rab11a in parietal cells and other epithelial cells. 2003, Pubmed
Law, The neural adhesion molecule TAG-1 modulates responses of sensory axons to diffusible guidance signals. 2008, Pubmed
Leterrier, Constitutive endocytic cycle of the CB1 cannabinoid receptor. 2004, Pubmed
Leung, Live visualization of protein synthesis in axonal growth cones by microinjection of photoconvertible Kaede into Xenopus embryos. 2008, Pubmed , Xenbase
Li, Identification of two functionally distinct endosomal recycling pathways for dopamine D₂ receptor. 2012, Pubmed
Lilienbaum, Chimeric integrins expressed in retinal ganglion cells impair process outgrowth in vivo. 1995, Pubmed , Xenbase
Liu, Nerve growth factor-mediated neurite outgrowth via regulation of Rab5. 2007, Pubmed
Mann, B-type Eph receptors and ephrins induce growth cone collapse through distinct intracellular pathways. 2003, Pubmed , Xenbase
Maxfield, Endocytic recycling. 2004, Pubmed
McCaffrey, Rab4 affects both recycling and degradative endosomal trafficking. 2001, Pubmed
McFarlane, Inhibition of FGF receptor activity in retinal ganglion cell axons causes errors in target recognition. 1996, Pubmed , Xenbase
Meijering, Design and validation of a tool for neurite tracing and analysis in fluorescence microscopy images. 2004, Pubmed
Miaczynska, Mosaic organization of the endocytic pathway. 2002, Pubmed
Mire, Spontaneous activity regulates Robo1 transcription to mediate a switch in thalamocortical axon growth. 2012, Pubmed
Mukhopadhyay, Rab7 regulates transport from early to late endocytic compartments in Xenopus oocytes. 1997, Pubmed , Xenbase
Nakazawa, Rab33a mediates anterograde vesicular transport for membrane exocytosis and axon outgrowth. 2012, Pubmed
Nawabi, A midline switch of receptor processing regulates commissural axon guidance in vertebrates. 2010, Pubmed
Nozumi, Identification of functional marker proteins in the mammalian growth cone. 2009, Pubmed
Otomo, ALS2/alsin deficiency in neurons leads to mild defects in macropinocytosis and axonal growth. 2008, Pubmed
Pagano, In vitro formation of recycling vesicles from endosomes requires adaptor protein-1/clathrin and is regulated by rab4 and the connector rabaptin-5. 2004, Pubmed
Palamidessi, Endocytic trafficking of Rac is required for the spatial restriction of signaling in cell migration. 2008, Pubmed
Panicker, Endocytosis of beta1 integrins is an early event in migration promoted by the cell adhesion molecule L1. 2006, Pubmed
Pei, Immune regulation of Rab proteins expression and intracellular transport. 2012, Pubmed
Pertz, Spatial mapping of the neurite and soma proteomes reveals a functional Cdc42/Rac regulatory network. 2008, Pubmed
Piper, Signaling mechanisms underlying Slit2-induced collapse of Xenopus retinal growth cones. 2006, Pubmed , Xenbase
Piper, Endocytosis-dependent desensitization and protein synthesis-dependent resensitization in retinal growth cone adaptation. 2005, Pubmed , Xenbase
Pittman, Pathfinding in a large vertebrate axon tract: isotypic interactions guide retinotectal axons at multiple choice points. 2008, Pubmed
Portera-Cailliau, Diverse modes of axon elaboration in the developing neocortex. 2005, Pubmed
Prigozhina, Decreased polarity and increased random motility in PtK1 epithelial cells correlate with inhibition of endosomal recycling. 2006, Pubmed
Raiborg, FYVE and coiled-coil domains determine the specific localisation of Hrs to early endosomes. 2001, Pubmed
Reid, Cycling of cell-surface MHC glycoproteins through primaquine-sensitive intracellular compartments. 1990, Pubmed
Ren, Hydrolysis of GTP on rab11 is required for the direct delivery of transferrin from the pericentriolar recycling compartment to the cell surface but not from sorting endosomes. 1998, Pubmed
Rink, Rab conversion as a mechanism of progression from early to late endosomes. 2005, Pubmed
Roberts, Dynamics of rab5 activation in endocytosis and phagocytosis. 2000, Pubmed
Roberts, PDGF-regulated rab4-dependent recycling of alphavbeta3 integrin from early endosomes is necessary for cell adhesion and spreading. 2001, Pubmed
Roman-Vendrell, Fast modulation of μ-opioid receptor (MOR) recycling is mediated by receptor agonists. 2012, Pubmed
Ruchhoeft, The neuronal architecture of Xenopus retinal ganglion cells is sculpted by rho-family GTPases in vivo. 1999, Pubmed , Xenbase
Sabo, Mobility and cycling of synaptic protein-containing vesicles in axonal growth cone filopodia. 2003, Pubmed
Sato, Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. 2005, Pubmed
Scapin, The crystal structure of the small GTPase Rab11b reveals critical differences relative to the Rab11a isoform. 2006, Pubmed
Schnatwinkel, The Rab5 effector Rabankyrin-5 regulates and coordinates different endocytic mechanisms. 2004, Pubmed
Schwartz, Recycling of the asialoglycoprotein receptor and the effect of lysosomotropic amines in hepatoma cells. 1984, Pubmed
Shirakawa, Small GTPase Rab4 regulates Ca2+-induced alpha-granule secretion in platelets. 2000, Pubmed
Sönnichsen, Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11. 2000, Pubmed
Stenmark, Rab GTPases as coordinators of vesicle traffic. 2009, Pubmed
Stenmark, The Rab GTPase family. 2001, Pubmed
Stenmark, Inhibition of rab5 GTPase activity stimulates membrane fusion in endocytosis. 1994, Pubmed
Stoorvogel, Sorting of endocytosed transferrin and asialoglycoprotein occurs immediately after internalization in HepG2 cells. 1987, Pubmed
Strachan, Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility. 2004, Pubmed
Tebar, Calmodulin regulates intracellular trafficking of epidermal growth factor receptor and the MAPK signaling pathway. 2002, Pubmed
Thelen, Ubiquitination and endocytosis of cell adhesion molecule DM-GRASP regulate its cell surface presence and affect its role for axon navigation. 2008, Pubmed
Tojima, Attractive axon guidance involves asymmetric membrane transport and exocytosis in the growth cone. 2007, Pubmed
Tojima, Asymmetric clathrin-mediated endocytosis drives repulsive growth cone guidance. 2010, Pubmed
Tomoda, Role of Unc51.1 and its binding partners in CNS axon outgrowth. 2004, Pubmed
Ullrich, Rab11 regulates recycling through the pericentriolar recycling endosome. 1996, Pubmed
Ulrich, Wnt11 functions in gastrulation by controlling cell cohesion through Rab5c and E-cadherin. 2005, Pubmed
van den Brink, Commissureless regulation of axon outgrowth across the midline is independent of Rab function. 2013, Pubmed
van der Sluijs, The small GTP-binding protein rab4 controls an early sorting event on the endocytic pathway. 1992, Pubmed
van Weert, Primaquine interferes with membrane recycling from endosomes to the plasma membrane through a direct interaction with endosomes which does not involve neutralisation of endosomal pH nor osmotic swelling of endosomes. 2000, Pubmed
Visel, GenePaint.org: an atlas of gene expression patterns in the mouse embryo. 2004, Pubmed
Ward, From sorting endosomes to exocytosis: association of Rab4 and Rab11 GTPases with the Fc receptor, FcRn, during recycling. 2005, Pubmed
Weigert, Rab22a regulates the recycling of membrane proteins internalized independently of clathrin. 2004, Pubmed
White, alpha v beta3 and alpha5beta1 integrin recycling pathways dictate downstream Rho kinase signaling to regulate persistent cell migration. 2007, Pubmed
Wilson, EEA1, a tethering protein of the early sorting endosome, shows a polarized distribution in hippocampal neurons, epithelial cells, and fibroblasts. 2000, Pubmed
Witze, Wnt5a control of cell polarity and directional movement by polarized redistribution of adhesion receptors. 2008, Pubmed
Woo, Rac1 and RhoA promote neurite outgrowth through formation and stabilization of growth cone point contacts. 2006, Pubmed , Xenbase
Woolworth, The Drosophila metastasis suppressor gene Nm23 homolog, awd, regulates epithelial integrity during oogenesis. 2009, Pubmed
Yamamoto, Functional cross-talk between Rab14 and Rab4 through a dual effector, RUFY1/Rabip4. 2010, Pubmed
Yoo, Endocytosis of EphA receptors is essential for the proper development of the retinocollicular topographic map. 2011, Pubmed
Zeigerer, Rab5 is necessary for the biogenesis of the endolysosomal system in vivo. 2012, Pubmed
Zhang, Thirty-one flavors of Drosophila rab proteins. 2007, Pubmed
Zhu, Rabex-5 is a Rab22 effector and mediates a Rab22-Rab5 signaling cascade in endocytosis. 2009, Pubmed
Zivraj, Subcellular profiling reveals distinct and developmentally regulated repertoire of growth cone mRNAs. 2010, Pubmed , Xenbase
Zolessi, Polarization and orientation of retinal ganglion cells in vivo. 2006, Pubmed