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BACKGROUND: Transcriptional regulation is normally based on the recognition by a transcription factor of a defined base sequence in a process of direct read-out. However, the nucleic acid secondary and tertiary structure can also act as a recognition site for the transcription factor in a process known as indirect read-out, although this is much less understood. We have previously identified such a transcriptional control mechanism in early Xenopus development where the interaction of the transcription factor ilf3 and the gata2 promoter requires the presence of both an unusual A-form DNA structure and a CCAAT sequence. Rapid identification of such promoters elsewhere in the Xenopus and other genomes would provide insight into a less studied area of gene regulation, although currently there are few tools to analyse genomes in such ways.
RESULTS: In this paper we report the implementation of a novel bioinformatics approach that has identified 86 such putative promoters in the Xenopus genome. We have shown that five of these sites are A-form in solution, bind to transcription factors and fully validated one of these newly identified promoters as interacting with the ilf3 containing complex CBTF. This interaction regulates the transcription of a previously uncharacterised downstream gene that is active in early development.
CONCLUSIONS: A Perl program (APTE) has located a number of potential A-form DNA promotor elements in the Xenopus genome, five of these putative targets have been experimentally validated as A-form and as targets for specific DNA binding proteins; one has also been shown to interact with the A-form binding transcription factor ilf3. APTE is available from http://www.port.ac.uk/research/cmd/software/ under the terms of the GNU General Public License.
Figure 1.
A combined promoter sequence consists of an A-form promoter element followed by a direct read-out promoter motif. The APE row indicates the signs of the APE values for the sequence in the Base row; with X denoting undetermined APE values [11]. The main parameters are the number of negative APE values in the APS (apelen), and the gap between the APS and the motif.
Figure 2.
The five selected sequences and their predicted binding proteins. Each of the putative promoter elements (ppe) sequences are within 500Â bp 5â² of the transcription start site of the genes-gdi3, gtf2, kif27, thrsp and unknown1, The key elements with potential gene regulatory function are underlined with grey arrows. The black arrow above each oligonucleotide indicates a putative transcription factor binding site and its direction of binding. The putative transcription factor binding sites were predicted using the EMBOSS database run through Geneious R7 7.1.4.
Figure 3.
The putative promoter element is A-form and binds ilf3
in vitro
. (a) Duplex 36Â bp oligonucleotides corresponding to the five identified putative promoter elements display A-form DNA characteristics as observed by circular dichroism. (b) These duplex oligonucletides are shifted in EMSA experiments, these complexes are competed by titration of unlabelled self-competitor but not by CCAAT box containing B-form duplexes. The specific complexes are indicated by arrows. (c) The sequence of the B-form competitor used in the EMSA is shown, the CCAAT box is indicated in bold.
Figure 4.
The expression of
gdi3
mRNA is maximal at neurula stage and can be modulated by ilf3. (a) The complex gdi3 specific complex can be supershifted by addition of anti-ilf3 antibody. (b) The gdi3 gene expression is zygotic and peaks at neurula stage 18 when ilf3 is known to be nuclear and active. (c) Expression of gdi3 is ablated relative to an internal control, ODC, by exogenous expression a dominant-negative form of ilf3 (ilf3en) which acts at the transcriptional level.
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