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Proc Natl Acad Sci U S A
2014 Nov 11;11145:16196-201. doi: 10.1073/pnas.1417473111.
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Nitric oxide negatively regulates AKT1-mediated potassium uptake through modulating vitamin B6 homeostasis in Arabidopsis.
Xia J
,
Kong D
,
Xue S
,
Tian W
,
Li N
,
Bao F
,
Hu Y
,
Du J
,
Wang Y
,
Pan X
,
Wang L
,
Zhang X
,
Niu G
,
Feng X
,
Li L
,
He Y
.
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Nitric oxide (NO), an active signaling molecule in plants, is involved in numerous physiological processes and adaptive responses to environmental stresses. Under high-salt conditions, plants accumulate NO quickly, and reorganize Na(+) and K(+) contents. However, the molecular connection between NO and ion homeostasis is largely unknown. Here, we report that NO lowers K(+) channel AKT1-mediated plant K(+) uptake by modulating vitamin B6 biosynthesis. In a screen for Arabidopsis NO-hypersensitive mutants, we isolated sno1 (sensitive to nitric oxide 1), which is allelic to the previously noted mutant sos4 (salt overly sensitive 4) that has impaired Na(+) and K(+) contents and overproduces pyridoxal 5'-phosphate (PLP), an active form of vitamin B6. We showed that NO increased PLP and decreased K(+) levels in plant. NO induced SNO1 gene expression and enzyme activity, indicating that NO-triggered PLP accumulation mainly occurs through SNO1-mediated vitamin B6 salvage biosynthetic pathway. Furthermore, we demonstrated that PLP significantly repressed the activity of K(+) channel AKT1 in the Xenopus oocyte system and Arabidopsis root protoplasts. Together, our results suggest that NO decreases K(+) absorption by promoting the synthesis of vitamin B6 PLP, which further represses the activity of K(+) channel AKT1 in Arabidopsis. These findings reveal a previously unidentified pivotal role of NO in modulating the homeostasis of vitamin B6 and potassium nutrition in plants, and shed light on the mechanism of NO in plant acclimation to environmental changes.
Alemán,
Root K(+) acquisition in plants: the Arabidopsis thaliana model.
2011, Pubmed
Alemán,
Root K(+) acquisition in plants: the Arabidopsis thaliana model.
2011,
Pubmed
Arasimowicz-Jelonek,
The message of nitric oxide in cadmium challenged plants.
2011,
Pubmed
Bai,
Deciphering the protective role of nitric oxide against salt stress at the physiological and proteomic levels in maize.
2011,
Pubmed
Ballatori,
ATP depletion and inactivation of an ATP-sensitive taurine channel by classic ion channel blockers.
1995,
Pubmed
Bethke,
Sodium nitroprusside, cyanide, nitrite, and nitrate break Arabidopsis seed dormancy in a nitric oxide-dependent manner.
2006,
Pubmed
Chen,
Pyridoxine is required for post-embryonic root development and tolerance to osmotic and oxidative stresses.
2005,
Pubmed
Chen,
Nitric oxide mediates root K+/Na+ balance in a mangrove plant, Kandelia obovata, by enhancing the expression of AKT1-type K+ channel and Na+/H+ antiporter under high salinity.
2013,
Pubmed
Chen,
Nitric oxide enhances salt secretion and Na(+) sequestration in a mangrove plant, Avicennia marina, through increasing the expression of H(+)-ATPase and Na(+)/H(+) antiporter under high salinity.
2010,
Pubmed
Cheong,
Two calcineurin B-like calcium sensors, interacting with protein kinase CIPK23, regulate leaf transpiration and root potassium uptake in Arabidopsis.
2007,
Pubmed
Chérel,
Regulation of K+ channel activities in plants: from physiological to molecular aspects.
2004,
Pubmed
Courtois,
Nitric oxide signalling in plants: interplays with Ca2+ and protein kinases.
2008,
Pubmed
Delledonne,
NO news is good news for plants.
2005,
Pubmed
Fitzpatrick,
Vitamin deficiencies in humans: can plant science help?
2012,
Pubmed
Fitzpatrick,
Two independent routes of de novo vitamin B6 biosynthesis: not that different after all.
2007,
Pubmed
Garcia-Mata,
Nitric oxide regulates K+ and Cl- channels in guard cells through a subset of abscisic acid-evoked signaling pathways.
2003,
Pubmed
García-Mata,
Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress.
2001,
Pubmed
Gaymard,
The baculovirus/insect cell system as an alternative to Xenopus oocytes. First characterization of the AKT1 K+ channel from Arabidopsis thaliana.
1996,
Pubmed
,
Xenbase
Geiger,
Heteromeric AtKC1{middle dot}AKT1 channels in Arabidopsis roots facilitate growth under K+-limiting conditions.
2009,
Pubmed
González,
Vitamer levels, stress response, enzyme activity, and gene regulation of Arabidopsis lines mutant in the pyridoxine/pyridoxamine 5'-phosphate oxidase (PDX3) and the pyridoxal kinase (SOS4) genes involved in the vitamin B6 salvage pathway.
2007,
Pubmed
Graziano,
Nitric oxide and iron in plants: an emerging and converging story.
2005,
Pubmed
Guo,
Arabidopsis nitric oxide synthase1 is targeted to mitochondria and protects against oxidative damage and dark-induced senescence.
2005,
Pubmed
He,
Interactions between nitric oxide and plant hormones in aluminum tolerance.
2012,
Pubmed
He,
Nitric oxide represses the Arabidopsis floral transition.
2004,
Pubmed
Hinz,
Lysine 480 is an essential residue in the putative ATP site of lamb kidney (Na,K)-ATPase. Identification of the pyridoxal 5'-diphospho-5'-adenosine and pyridoxal phosphate reactive residue.
1990,
Pubmed
Hirsch,
A role for the AKT1 potassium channel in plant nutrition.
1998,
Pubmed
Lagarde,
Tissue-specific expression of Arabidopsis AKT1 gene is consistent with a role in K+ nutrition.
1996,
Pubmed
Lamattina,
Nitric oxide: the versatility of an extensive signal molecule.
2003,
Pubmed
Lee,
A protein phosphorylation/dephosphorylation network regulates a plant potassium channel.
2007,
Pubmed
Leitner,
NO signals in the haze: nitric oxide signalling in plant defence.
2009,
Pubmed
Li,
A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis.
2006,
Pubmed
,
Xenbase
Libourel,
Nitric oxide gas stimulates germination of dormant Arabidopsis seeds: use of a flow-through apparatus for delivery of nitric oxide.
2006,
Pubmed
Mishina,
Expression of a nitric oxide degrading enzyme induces a senescence programme in Arabidopsis.
2007,
Pubmed
Molassiotis,
NO says more than 'YES' to salt tolerance: Salt priming and systemic nitric oxide signaling in plants.
2010,
Pubmed
Mur,
Nitric oxide in plants: an assessment of the current state of knowledge.
2013,
Pubmed
Neill,
Nitric oxide, stomatal closure, and abiotic stress.
2008,
Pubmed
Nieves-Cordones,
The Arabidopsis thaliana HAK5 K+ transporter is required for plant growth and K+ acquisition from low K+ solutions under saline conditions.
2010,
Pubmed
Pagnussat,
Nitric oxide is required for root organogenesis.
2002,
Pubmed
Palmgren,
PLANT PLASMA MEMBRANE H+-ATPases: Powerhouses for Nutrient Uptake.
2001,
Pubmed
Pyo,
High-affinity K(+) transport in Arabidopsis: AtHAK5 and AKT1 are vital for seedling establishment and postgermination growth under low-potassium conditions.
2010,
Pubmed
Qiao,
Nitric oxide signaling in plant responses to abiotic stresses.
2008,
Pubmed
Ralevic,
Receptors for purines and pyrimidines.
1998,
Pubmed
Ramirez,
Nitric oxide, nitrosyl iron complexes, ferritin and frataxin: a well equipped team to preserve plant iron homeostasis.
2011,
Pubmed
Ribeiro,
Differential requirement for NO during ABA-induced stomatal closure in turgid and wilted leaves.
2009,
Pubmed
Rubio,
Studies on Arabidopsis athak5, atakt1 double mutants disclose the range of concentrations at which AtHAK5, AtAKT1 and unknown systems mediate K uptake.
2010,
Pubmed
Schachtman,
Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA.
1992,
Pubmed
,
Xenbase
Sentenac,
Cloning and expression in yeast of a plant potassium ion transport system.
1992,
Pubmed
Shi,
The Arabidopsis salt overly sensitive 4 mutants uncover a critical role for vitamin B6 in plant salt tolerance.
2002,
Pubmed
Šírová,
The role of nitric oxide in the germination of plant seeds and pollen.
2011,
Pubmed
Sokolovski,
Nitric oxide block of outward-rectifying K+ channels indicates direct control by protein nitrosylation in guard cells.
2004,
Pubmed
Sokolovski,
Protein phosphorylation is a prerequisite for intracellular Ca2+ release and ion channel control by nitric oxide and abscisic acid in guard cells.
2005,
Pubmed
Tambasco-Studart,
Vitamin B6 biosynthesis in higher plants.
2005,
Pubmed
Tanaka,
Evolution of vitamin B6 (pyridoxine) metabolism by gain and loss of genes.
2005,
Pubmed
Terrile,
Nitric oxide influences auxin signaling through S-nitrosylation of the Arabidopsis TRANSPORT INHIBITOR RESPONSE 1 auxin receptor.
2012,
Pubmed
Wang,
Ethylene and nitric oxide are involved in maintaining ion homeostasis in Arabidopsis callus under salt stress.
2009,
Pubmed
Wang,
Potassium channel alpha-subunit AtKC1 negatively regulates AKT1-mediated K(+) uptake in Arabidopsis roots under low-K(+) stress.
2010,
Pubmed
Wang,
Potassium transport and signaling in higher plants.
2013,
Pubmed
Xu,
A protein kinase, interacting with two calcineurin B-like proteins, regulates K+ transporter AKT1 in Arabidopsis.
2006,
Pubmed
,
Xenbase
Zhang,
Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of proton-pump and Na+/H+ antiport in the tonoplast.
2006,
Pubmed
Zhao,
Nitric oxide functions as a signal in salt resistance in the calluses from two ecotypes of reed.
2004,
Pubmed
Zhao,
Nitric oxide synthase-dependent nitric oxide production is associated with salt tolerance in Arabidopsis.
2007,
Pubmed
Zhu,
Genetic analysis of salt tolerance in arabidopsis. Evidence for a critical role of potassium nutrition.
1998,
Pubmed
Zhu,
Regulation of ion homeostasis under salt stress.
2003,
Pubmed
