Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Transcription activator-like effector nucleases (TALENs) have previously been used for targeted genome editing in various organisms including Xenopus laevis. However, because of genomic polyploidization, X. laevis usually possess homeologous genes (homeologs) with quite similar sequences that make the analysis of gene function difficult. In the present study, we show methodological examples of targeted gene modification of X. laevis homeologs. The X. laevis cytoglobin gene (cygb) consists of two homeologs (xlcygba and xlcygbb), and molecular phylogenetic analysis suggested that they have potentially different functions. Thus, there is a need to establish a method of homeolog-specific gene disruption to clarify gene functions in detail. Here, we show successful examples of homeolog-specific and simultaneous gene disruption for xlcygba and xlcygbb. We found that selective digestion can be performed with at least three mismatches in TALEN target sites in both homeologs. This report paves the way for the functional analyses of X. laevis homeologs, even those containing nearly identical sequences.
Abascal,
TranslatorX: multiple alignment of nucleotide sequences guided by amino acid translations.
2010, Pubmed
Abascal,
TranslatorX: multiple alignment of nucleotide sequences guided by amino acid translations.
2010,
Pubmed
Castresana,
Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis.
2000,
Pubmed
Cermak,
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting.
2011,
Pubmed
Chain,
Multiple mechanisms promote the retained expression of gene duplicates in the tetraploid frog Xenopus laevis.
2006,
Pubmed
,
Xenbase
Evans,
A mitochondrial DNA phylogeny of African clawed frogs: phylogeography and implications for polyploid evolution.
2004,
Pubmed
,
Xenbase
Fuchs,
Duplicated cytoglobin genes in teleost fishes.
2005,
Pubmed
Jones,
The rapid generation of mutation data matrices from protein sequences.
1992,
Pubmed
Joung,
TALENs: a widely applicable technology for targeted genome editing.
2013,
Pubmed
Katoh,
MAFFT version 5: improvement in accuracy of multiple sequence alignment.
2005,
Pubmed
Le,
An improved general amino acid replacement matrix.
2008,
Pubmed
Mali,
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
2013,
Pubmed
Miller,
A TALE nuclease architecture for efficient genome editing.
2011,
Pubmed
Miyanari,
Live visualization of chromatin dynamics with fluorescent TALEs.
2013,
Pubmed
Nakade,
Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9.
2014,
Pubmed
,
Xenbase
Ochiai,
Targeted mutagenesis in the sea urchin embryo using zinc-finger nucleases.
2010,
Pubmed
Ronquist,
MrBayes 3: Bayesian phylogenetic inference under mixed models.
2003,
Pubmed
Sakane,
Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases.
2014,
Pubmed
,
Xenbase
Sakuma,
Efficient TALEN construction and evaluation methods for human cell and animal applications.
2013,
Pubmed
,
Xenbase
Sakuma,
Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity.
2013,
Pubmed
,
Xenbase
Sakuma,
Nuclease-mediated genome editing: At the front-line of functional genomics technology.
2014,
Pubmed
Suzuki,
High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos.
2013,
Pubmed
,
Xenbase
Tanabe,
Kakusan4 and Aminosan: two programs for comparing nonpartitioned, proportional and separate models for combined molecular phylogenetic analyses of multilocus sequence data.
2011,
Pubmed
Uno,
Homoeologous chromosomes of Xenopus laevis are highly conserved after whole-genome duplication.
2013,
Pubmed
,
Xenbase
Xi,
Gene expression and tissue distribution of cytoglobin and myoglobin in the Amphibia and Reptilia: possible compensation of myoglobin with cytoglobin in skeletal muscle cells of anurans that lack the myoglobin gene.
2007,
Pubmed
,
Xenbase
Yoshimi,
Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform.
2014,
Pubmed
