Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Channels (Austin)
2015 Jan 01;93:121-8. doi: 10.1080/19336950.2015.1036205.
Show Gene links
Show Anatomy links
PKC and AMPK regulation of Kv1.5 potassium channels.
Andersen MN
,
Skibsbye L
,
Tang C
,
Petersen F
,
MacAulay N
,
Rasmussen HB
,
Jespersen T
.
???displayArticle.abstract???
The voltage-gated Kv1.5 potassium channel, conducting the ultra-rapid rectifier K(+) current (IKur), is regulated through several pathways. Here we investigate if Kv1.5 surface expression is controlled by the 2 kinases PKC and AMPK, using Xenopus oocytes, MDCK cells and atrial derived HL-1 cells. By confocal microscopy combined with electrophysiology we demonstrate that PKC activation reduces Kv1.5 current, through a decrease in membrane expressed channels. AMPK activation was found to decrease the membrane expression in MDCK cells, but not in HL-1 cells and was furthermore shown to be dependent on co-expression of Nedd4-2 in Xenopus oocytes. These results indicate that Kv1.5 channels are regulated by both kinases, although through different molecular mechanisms in different cell systems.
Adda,
Expression and function of voltage-dependent potassium channel genes in human airway smooth muscle.
1996, Pubmed
Adda,
Expression and function of voltage-dependent potassium channel genes in human airway smooth muscle.
1996,
Pubmed
Alesutan,
Inhibition of the heterotetrameric K+ channel KCNQ1/KCNE1 by the AMP-activated protein kinase.
2011,
Pubmed
,
Xenbase
Alzamora,
AMP-activated protein kinase inhibits KCNQ1 channels through regulation of the ubiquitin ligase Nedd4-2 in renal epithelial cells.
2010,
Pubmed
,
Xenbase
Andersen,
Kv7.1 surface expression is regulated by epithelial cell polarization.
2011,
Pubmed
Andersen,
AMP-activated protein kinase downregulates Kv7.1 cell surface expression.
2012,
Pubmed
,
Xenbase
Andersen,
AMPK: A regulator of ion channels.
2012,
Pubmed
Andersen,
A phosphoinositide 3-kinase (PI3K)-serum- and glucocorticoid-inducible kinase 1 (SGK1) pathway promotes Kv7.1 channel surface expression by inhibiting Nedd4-2 protein.
2013,
Pubmed
Bielanska,
Voltage-dependent potassium channels Kv1.3 and Kv1.5 in human fetus.
2010,
Pubmed
Bielanska,
Voltage-dependent potassium channels Kv1.3 and Kv1.5 in human cancer.
2009,
Pubmed
Boehmer,
Modulation of the voltage-gated potassium channel Kv1.5 by the SGK1 protein kinase involves inhibition of channel ubiquitination.
2008,
Pubmed
,
Xenbase
Brouillette,
Functional properties of K+ currents in adult mouse ventricular myocytes.
2004,
Pubmed
Christophersen,
Genetic variation in KCNA5: impact on the atrial-specific potassium current IKur in patients with lone atrial fibrillation.
2013,
Pubmed
Claycomb,
HL-1 cells: a cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte.
1998,
Pubmed
David,
Protein kinase C (PKC) activity regulates functional effects of Kvβ1.3 subunit on KV1.5 channels: identification of a cardiac Kv1.5 channelosome.
2012,
Pubmed
Fischer,
Isoenzyme-specific regulation of cardiac Kv1.5/Kvβ1.2 ion channel complex by protein kinase C: central role of PKCβII.
2014,
Pubmed
,
Xenbase
Ford,
Human electrophysiological and pharmacological properties of XEN-D0101: a novel atrial-selective Kv1.5/IKur inhibitor.
2013,
Pubmed
Grunnet,
Apamin interacts with all subtypes of cloned small-conductance Ca2+-activated K+ channels.
2001,
Pubmed
,
Xenbase
Jespersen,
The KCNQ1 potassium channel is down-regulated by ubiquitylating enzymes of the Nedd4/Nedd4-like family.
2007,
Pubmed
Kang,
Aging and muscle fiber type alter K+ channel contributions to the myogenic response in skeletal muscle arterioles.
2009,
Pubmed
Kwak,
Phosphorylation is required for alteration of kv1.5 K(+) channel function by the Kvbeta1.3 subunit.
1999,
Pubmed
Kwak,
Protein kinase A phosphorylation alters Kvbeta1.3 subunit-mediated inactivation of the Kv1.5 potassium channel.
1999,
Pubmed
,
Xenbase
Lang,
On the putative purpose of AMPK sensitive Kv1.5 K(+) channel regulation.
2015,
Pubmed
Li,
KChIP2 modulates the cell surface expression of Kv 1.5-encoded K(+) channels.
2005,
Pubmed
Lundby,
Effect of the I(to) activator NS5806 on cloned K(V)4 channels depends on the accessory protein KChIP2.
2010,
Pubmed
,
Xenbase
MacDonald,
Voltage-dependent K(+) channels in pancreatic beta cells: role, regulation and potential as therapeutic targets.
2003,
Pubmed
Mia,
Downregulation of Kv1.5 K channels by the AMP-activated protein kinase.
2012,
Pubmed
,
Xenbase
Olson,
Kv1.5 channelopathy due to KCNA5 loss-of-function mutation causes human atrial fibrillation.
2006,
Pubmed
Pang,
Small molecule antagonizes autoinhibition and activates AMP-activated protein kinase in cells.
2008,
Pubmed
Plane,
Heteromultimeric Kv1 channels contribute to myogenic control of arterial diameter.
2005,
Pubmed
Ravens,
Ultra-rapid delayed rectifier channels: molecular basis and therapeutic implications.
2011,
Pubmed
Thorneloe,
Molecular composition of 4-aminopyridine-sensitive voltage-gated K(+) channels of vascular smooth muscle.
2001,
Pubmed
Uebele,
Distinct domains of the voltage-gated K+ channel Kv beta 1.3 beta-subunit affect voltage-dependent gating.
1998,
Pubmed
,
Xenbase
Uebele,
Functional differences in Kv1.5 currents expressed in mammalian cell lines are due to the presence of endogenous Kv beta 2.1 subunits.
1996,
Pubmed
Wang,
Sustained depolarization-induced outward current in human atrial myocytes. Evidence for a novel delayed rectifier K+ current similar to Kv1.5 cloned channel currents.
1993,
Pubmed
Williams,
Modulation of the human Kv1.5 channel by protein kinase C activation: role of the Kvbeta1.2 subunit.
2002,
Pubmed
