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FASEB J
2015 Dec 01;2912:4866-80. doi: 10.1096/fj.15-275677.
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Kinesin family 17 (osmotic avoidance abnormal-3) is dispensable for photoreceptor morphology and function.
Jiang L
,
Tam BM
,
Ying G
,
Wu S
,
Hauswirth WW
,
Frederick JM
,
Moritz OL
,
Baehr W
.
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In Caenorhabditis elegans, homodimeric [kinesin family (KIF) 17, osmotic avoidance abnormal-3 (OSM-3)] and heterotrimeric (KIF3) kinesin-2 motors are required to establish sensory cilia by intraflagellar transport (IFT) where KIF3 and KIF17 cooperate to build the axoneme core and KIF17 builds the distal segments. However, the function of KIF17 in vertebrates is unresolved. We expressed full-length and motorless KIF17 constructs in mouse rod photoreceptors using adeno-associated virus in Xenopus laevis rod photoreceptors using a transgene and in ciliated IMCD3 cells. We found that tagged KIF17 localized along the rod outer segment axoneme when expressed in mouse and X. laevis photoreceptors, whereas KIF3A was restricted to the proximalaxoneme. Motorless KIF3A and KIF17 mutants caused photoreceptor degeneration, likely through dominant negative effects on IFT. KIF17 mutant lacking the motor domain translocated to nuclei after exposure of a C-terminal nuclear localization signal. Germ-line deletion of Kif17 in mouse did not affect photoreceptor function. A rod-specific Kif3/Kif17 double knockout mouse demonstrated that KIF17 and KIF3 do not act synergistically and did not prevent rhodopsin trafficking to rod outer segments. In summary, the nematode model of KIF3/KIF17 cooperation apparently does not apply to mouse photoreceptors in which the photosensory cilium is built exclusively by KIF3.
Audas,
The nucleolar detention pathway: A cellular strategy for regulating molecular networks.
2012, Pubmed
Audas,
The nucleolar detention pathway: A cellular strategy for regulating molecular networks.
2012,
Pubmed
Avasthi,
Trafficking of membrane proteins to cone but not rod outer segments is dependent on heterotrimeric kinesin-II.
2009,
Pubmed
Bader,
Analysis of KIF17 distal tip trafficking in zebrafish cone photoreceptors.
2012,
Pubmed
Chu,
A role for Kif17 in transport of Kv4.2.
2006,
Pubmed
Chuang,
SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods.
2007,
Pubmed
Deretic,
Molecular assemblies that control rhodopsin transport to the cilia.
2012,
Pubmed
DerMardirossian,
GDIs: central regulatory molecules in Rho GTPase activation.
2005,
Pubmed
Dishinger,
Ciliary entry of the kinesin-2 motor KIF17 is regulated by importin-beta2 and RanGTP.
2010,
Pubmed
Evans,
Functional modulation of IFT kinesins extends the sensory repertoire of ciliated neurons in Caenorhabditis elegans.
2006,
Pubmed
Gilliam,
Three-dimensional architecture of the rod sensory cilium and its disruption in retinal neurodegeneration.
2012,
Pubmed
Guillaud,
Disruption of KIF17-Mint1 interaction by CaMKII-dependent phosphorylation: a molecular model of kinesin-cargo release.
2008,
Pubmed
Hammond,
Autoinhibition of the kinesin-2 motor KIF17 via dual intramolecular mechanisms.
2010,
Pubmed
Imanishi,
Autoinhibition regulates the motility of the C. elegans intraflagellar transport motor OSM-3.
2006,
Pubmed
Inglis,
The sensory cilia of Caenorhabditis elegans.
2007,
Pubmed
Insinna,
Analysis of IFT kinesins in developing zebrafish cone photoreceptor sensory cilia.
2009,
Pubmed
Insinna,
Different roles for KIF17 and kinesin II in photoreceptor development and maintenance.
2009,
Pubmed
Insinna,
The homodimeric kinesin, Kif17, is essential for vertebrate photoreceptor sensory outer segment development.
2008,
Pubmed
Jenkins,
Ciliary targeting of olfactory CNG channels requires the CNGB1b subunit and the kinesin-2 motor protein, KIF17.
2006,
Pubmed
Jiang,
Heterotrimeric kinesin-2 (KIF3) mediates transition zone and axoneme formation of mouse photoreceptors.
2015,
Pubmed
Jiang,
Long-term RNA interference gene therapy in a dominant retinitis pigmentosa mouse model.
2011,
Pubmed
Karan,
Targeting of mouse guanylate cyclase 1 (Gucy2e) to Xenopus laevis rod outer segments.
2011,
Pubmed
,
Xenbase
Kayadjanian,
Localization of glutamate receptors to distal dendrites depends on subunit composition and the kinesin motor protein KIF17.
2007,
Pubmed
Kroll,
Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation.
1996,
Pubmed
,
Xenbase
Liu,
Photoreceptor sensory cilia and inherited retinal degeneration.
2010,
Pubmed
Malicki,
Kinesin-2 family motors in the unusual photoreceptor cilium.
2012,
Pubmed
Marszalek,
Genetic evidence for selective transport of opsin and arrestin by kinesin-II in mammalian photoreceptors.
2000,
Pubmed
Miyoshi,
Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice.
2009,
Pubmed
Moritz,
Selection of transgenic Xenopus laevis using antibiotic resistance.
2002,
Pubmed
,
Xenbase
Pan,
Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors.
2006,
Pubmed
Scholey,
Kinesin-2 motors transport IFT-particles, dyneins and tubulin subunits to the tips of Caenorhabditis elegans sensory cilia: relevance to vision research?
2012,
Pubmed
Scholey,
Kinesin-2: a family of heterotrimeric and homodimeric motors with diverse intracellular transport functions.
2013,
Pubmed
Setou,
Kinesin superfamily motor protein KIF17 and mLin-10 in NMDA receptor-containing vesicle transport.
2000,
Pubmed
Signor,
Two heteromeric kinesin complexes in chemosensory neurons and sensory cilia of Caenorhabditis elegans.
1999,
Pubmed
Signor,
Role of a class DHC1b dynein in retrograde transport of IFT motors and IFT raft particles along cilia, but not dendrites, in chemosensory neurons of living Caenorhabditis elegans.
1999,
Pubmed
Snow,
Two anterograde intraflagellar transport motors cooperate to build sensory cilia on C. elegans neurons.
2004,
Pubmed
Solovei,
Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolution.
2009,
Pubmed
Tam,
Mislocalized rhodopsin does not require activation to cause retinal degeneration and neurite outgrowth in Xenopus laevis.
2006,
Pubmed
,
Xenbase
Verhey,
Traffic control: regulation of kinesin motors.
2009,
Pubmed
Wolfrum,
Rhodopsin transport in the membrane of the connecting cilium of mammalian photoreceptor cells.
2000,
Pubmed
Wu,
A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond.
2008,
Pubmed
Ye,
Single molecule imaging reveals a major role for diffusion in the exploration of ciliary space by signaling receptors.
2013,
Pubmed
Yin,
Molecular motor KIF17 is fundamental for memory and learning via differential support of synaptic NR2A/2B levels.
2011,
Pubmed
Young,
The renewal of photoreceptor cell outer segments.
1967,
Pubmed
Zhao,
Kinesin-2 family in vertebrate ciliogenesis.
2012,
Pubmed
Zolotukhin,
Recombinant adeno-associated virus purification using novel methods improves infectious titer and yield.
1999,
Pubmed
