Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-5123
Dev Dyn 2003 Jul 01;2273:445-9. doi: 10.1002/dvdy.10318.
Show Gene links Show Anatomy links

Expression of MRF4 protein in adult and in regenerating muscles in Xenopus.

Becker C , Della Gaspera B , Guyot M , Donsez E , Armand AS , Charbonnier F , Launay T , Chanoine C .


???displayArticle.abstract???
In Xenopus, previous studies showed that the transcripts of the myogenic regulatory factor (MRF) MRF4 accumulate during skeletal muscle differentiation, but nothing is known about the accumulation of XMRF4 protein during myogenesis. In this report, an affinity-purified polyclonal antibody against Xenopus MRF4 was developed and used to describe the pattern of expression of this myogenic factor in the adult and in regenerating muscles. From young forming myotubes, XMRF4 protein persistently accumulated in nuclei during the regeneration process and was strongly expressed in nuclei of adult muscles. No selective accumulation of XMRF4 protein was detectable at neuromuscular junctions, but XMRF4 immunoreactivity was observed in sole plate nuclei as well as in extrasynaptic myofiber nuclei. We also report that XMRF4 protein accumulated before the establishment of neuromuscular connections, showing that innervation is not necessary for the appearance of XMRF4 protein during muscle regeneration.

???displayArticle.pubmedLink??? 12815631
???displayArticle.link??? Dev Dyn


Species referenced: Xenopus
Genes referenced: myf6
???displayArticle.antibodies??? Myf6 Ab1