XB-ART-51303
Adv Exp Med Biol
2015 Jan 01;869:119-51. doi: 10.1007/978-1-4939-2845-3_7.
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Incorporation of Non-Canonical Amino Acids.
Leisle L
,
Valiyaveetil F
,
Mehl RA
,
Ahern CA
.
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In this chapter we discuss the strengths, caveats and technical considerations of three approaches for reprogramming the chemical composition of selected amino acids within a membrane protein. In vivo nonsense suppression in the Xenopus laevis oocyte, evolved orthogonal tRNA and aminoacyl-tRNA synthetase pairs and protein ligation for biochemical production of semisynthetic proteins have been used successfully for ion channel and receptor studies. The level of difficulty for the application of each approach ranges from trivial to technically demanding, yet all have untapped potential in their application to membrane proteins.
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R01 GM106569 NIGMS NIH HHS , R01 GM114653 NIGMS NIH HHS , R21 AR066802 NIAMS NIH HHS , R01 GM087546 NIGMS NIH HHS
Genes referenced: mt-tr trna
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