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The Rpd3S histone deacetylase complex utilizes two subunits, Eaf3 and Rco1, to recognize nucleosomes methylated at H3K36 (H3K36me) with high affinity and strong specificity. However, the chromobarrel domain of Eaf3 (CHD) that is responsible for H3K36me recognition only binds weakly and with little specificity to histone peptides. Here, using deuterium exchange mass spectrometry (DXMS), we detected conformational changes of Rpd3S upon its contact with chromatin. Interestingly, we found that the Sin3-interacting domain of Rco1 (SID) allosterically stimulates preferential binding of Eaf3 to H3K36-methylated peptides. This activation is tightly regulated by an autoinhibitory mechanism to ensure optimal multivalent engagement of Rpd3S with nucleosomes. Lastly, we identified mutations at the interface between SID and Eaf3 that do not disrupt complex integrity but severely compromise Rpd3S functions in vitro and in vivo, suggesting that the nucleosome-induced conformational changes are essential for chromatin recognition.
Biswas,
Different genetic functions for the Rpd3(L) and Rpd3(S) complexes suggest competition between NuA4 and Rpd3(S).
2008, Pubmed
Biswas,
Different genetic functions for the Rpd3(L) and Rpd3(S) complexes suggest competition between NuA4 and Rpd3(S).
2008,
Pubmed
Burns-Hamuro,
Distinct interaction modes of an AKAP bound to two regulatory subunit isoforms of protein kinase A revealed by amide hydrogen/deuterium exchange.
2005,
Pubmed
Carrozza,
Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription.
2005,
Pubmed
Cheung,
Chromatin- and transcription-related factors repress transcription from within coding regions throughout the Saccharomyces cerevisiae genome.
2008,
Pubmed
Drouin,
DSIF and RNA polymerase II CTD phosphorylation coordinate the recruitment of Rpd3S to actively transcribed genes.
2010,
Pubmed
Engen,
Analysis of protein conformation and dynamics by hydrogen/deuterium exchange MS.
2009,
Pubmed
Govind,
Phosphorylated Pol II CTD recruits multiple HDACs, including Rpd3C(S), for methylation-dependent deacetylation of ORF nucleosomes.
2010,
Pubmed
Huh,
Multivalent di-nucleosome recognition enables the Rpd3S histone deacetylase complex to tolerate decreased H3K36 methylation levels.
2012,
Pubmed
,
Xenbase
Jenuwein,
Translating the histone code.
2001,
Pubmed
Joshi,
Eaf3 chromodomain interaction with methylated H3-K36 links histone deacetylation to Pol II elongation.
2005,
Pubmed
Keogh,
Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex.
2005,
Pubmed
Kouzarides,
Chromatin modifications and their function.
2007,
Pubmed
Kumar,
Sequence requirements for combinatorial recognition of histone H3 by the MRG15 and Pf1 subunits of the Rpd3S/Sin3S corepressor complex.
2012,
Pubmed
Lee,
Chromatin remodelers fine-tune H3K36me-directed deacetylation of neighbor nucleosomes by Rpd3S.
2013,
Pubmed
Li,
The role of chromatin during transcription.
2007,
Pubmed
Li,
Infrequently transcribed long genes depend on the Set2/Rpd3S pathway for accurate transcription.
2007,
Pubmed
Li,
Combined action of PHD and chromo domains directs the Rpd3S HDAC to transcribed chromatin.
2007,
Pubmed
Margueron,
Role of the polycomb protein EED in the propagation of repressive histone marks.
2009,
Pubmed
Martino,
Reconstitution of yeast silent chromatin: multiple contact sites and O-AADPR binding load SIR complexes onto nucleosomes in vitro.
2009,
Pubmed
Murzina,
Structural basis for the recognition of histone H4 by the histone-chaperone RbAp46.
2008,
Pubmed
Ranjan,
Nucleosome-free region dominates histone acetylation in targeting SWR1 to promoters for H2A.Z replacement.
2013,
Pubmed
Ruthenburg,
Multivalent engagement of chromatin modifications by linked binding modules.
2007,
Pubmed
Shi,
Proteome-wide analysis in Saccharomyces cerevisiae identifies several PHD fingers as novel direct and selective binding modules of histone H3 methylated at either lysine 4 or lysine 36.
2007,
Pubmed
Sun,
Molecular basis of the interaction of Saccharomyces cerevisiae Eaf3 chromo domain with methylated H3K36.
2008,
Pubmed
Trojer,
L3MBTL1, a histone-methylation-dependent chromatin lock.
2007,
Pubmed
Tsai,
TRIM24 links a non-canonical histone signature to breast cancer.
2010,
Pubmed
Wen,
ZMYND11 links histone H3.3K36me3 to transcription elongation and tumour suppression.
2014,
Pubmed
Xie,
Structural basis for molecular interactions involving MRG domains: implications in chromatin biology.
2012,
Pubmed
Xu,
Structural basis for the recognition of methylated histone H3K36 by the Eaf3 subunit of histone deacetylase complex Rpd3S.
2008,
Pubmed
Yuan,
Dense chromatin activates Polycomb repressive complex 2 to regulate H3 lysine 27 methylation.
2012,
Pubmed
Yun,
Reconstitution of modified chromatin templates for in vitro functional assays.
2012,
Pubmed
Yun,
Readers of histone modifications.
2011,
Pubmed
