Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Anal Chem
2010 Feb 01;823:982-8. doi: 10.1021/ac902245p.
Show Gene links
Show Anatomy links
Simultaneous imaging of small metabolites and lipids in rat brain tissues at atmospheric pressure by laser ablation electrospray ionization mass spectrometry.
???displayArticle.abstract???
Atmospheric pressure imaging mass spectrometry is a rapidly expanding field that offers advantages in the ability to study biological systems in their native condition, simplified sample preparation, and high-throughput experiments. In laser ablation electrospray ionization (LAESI), the native water molecules in biological tissues facilitate sampling by a focused mid-infrared laser beam. The ionization of the ablated material is accomplished by electrospray postionization. In this work, we demonstrate that the imaging variant of LAESI simultaneously provides lateral distributions for small metabolites and lipids directly in rat brain sections. To cope with the fragile nature and potential dehydration of the braintissue due to drying in the ambient environment as well as to minimize analyte redistribution, a Peltier cooling stage is integrated into the LAESI imaging system. We demonstrate the utility of high-resolution (m/Deltam > 6000) time-of-flight mass spectrometry with LAESI to deconvolute spatial distributions of different chemical species with identical nominal mass. To help with the evaluation of the massive data sets, Pearson colocalization maps are calculated for selected small metabolites and lipids. We show that this approach reveals biologically meaningful correlations between these two classes of biomolecules.
Altelaar,
Direct molecular imaging of Lymnaea stagnalis nervous tissue at subcellular spatial resolution by mass spectrometry.
2005, Pubmed
Altelaar,
Direct molecular imaging of Lymnaea stagnalis nervous tissue at subcellular spatial resolution by mass spectrometry.
2005,
Pubmed
Andersson,
Imaging mass spectrometry of proteins and peptides: 3D volume reconstruction.
2008,
Pubmed
Benabdellah,
In situ primary metabolites localization on a rat brain section by chemical mass spectrometry imaging.
2009,
Pubmed
Brunelle,
Lipid imaging with cluster time-of-flight secondary ion mass spectrometry.
2009,
Pubmed
Chen,
Ambient imaging mass spectrometry by electrospray ionization using solid needle as sampling probe.
2009,
Pubmed
Cody,
Versatile new ion source for the analysis of materials in open air under ambient conditions.
2005,
Pubmed
Cooks,
Detection Technologies. Ambient mass spectrometry.
2006,
Pubmed
Cornett,
MALDI imaging mass spectrometry: molecular snapshots of biochemical systems.
2007,
Pubmed
Esquenazi,
Probing marine natural product defenses with DESI-imaging mass spectrometry.
2009,
Pubmed
Jackson,
Direct tissue analysis of phospholipids in rat brain using MALDI-TOFMS and MALDI-ion mobility-TOFMS.
2005,
Pubmed
Khatib-Shahidi,
Direct molecular analysis of whole-body animal tissue sections by imaging MALDI mass spectrometry.
2006,
Pubmed
Li,
A syntaxin 1, Galpha(o), and N-type calcium channel complex at a presynaptic nerve terminal: analysis by quantitative immunocolocalization.
2004,
Pubmed
Li,
Atmospheric pressure infrared MALDI imaging mass spectrometry for plant metabolomics.
2008,
Pubmed
Li,
Atmospheric pressure molecular imaging by infrared MALDI mass spectrometry.
2007,
Pubmed
Lin,
Influence of creatine and ethanolamine on the phosphate metabolism of rabbit cerebral cortex slices in vitro.
1966,
Pubmed
McDonnell,
Mass spectrometry image correlation: quantifying colocalization.
2008,
Pubmed
McDonnell,
Imaging mass spectrometry.
2007,
Pubmed
Mikawa,
Imaging of phosphatidylcholines in the adult rat brain using MALDI-TOF MS.
2009,
Pubmed
Munn,
Deficiency in ethanolamine plasmalogen leads to altered cholesterol transport.
2003,
Pubmed
Murphy,
Imaging of lipid species by MALDI mass spectrometry.
2009,
Pubmed
Nemes,
Three-dimensional imaging of metabolites in tissues under ambient conditions by laser ablation electrospray ionization mass spectrometry.
2009,
Pubmed
Nemes,
Laser ablation electrospray ionization for atmospheric pressure, in vivo, and imaging mass spectrometry.
2007,
Pubmed
Nemes,
Ambient molecular imaging and depth profiling of live tissue by infrared laser ablation electrospray ionization mass spectrometry.
2008,
Pubmed
Northen,
Clathrate nanostructures for mass spectrometry.
2007,
Pubmed
Ostrowski,
Mass spectrometric imaging of highly curved membranes during Tetrahymena mating.
2004,
Pubmed
Rubakhin,
Imaging mass spectrometry: fundamentals and applications to drug discovery.
2005,
Pubmed
Schuber,
Influence of polyamines on membrane functions.
1989,
Pubmed
Seiler,
Regional distribution of putrescine, spermidine and spermine in relation to the distribution of RNA and DNA in the rat nervous system.
1975,
Pubmed
Shaw,
The regional distribution of the polyamines spermidine and spermine in brain.
1973,
Pubmed
Shrestha,
In situ metabolic profiling of single cells by laser ablation electrospray ionization mass spectrometry.
2009,
Pubmed
Sjövall,
Mass spectrometric imaging of lipids in brain tissue.
2004,
Pubmed
Takáts,
Mass spectrometry sampling under ambient conditions with desorption electrospray ionization.
2004,
Pubmed
Touboul,
Tissue molecular ion imaging by gold cluster ion bombardment.
2004,
Pubmed
Wiseman,
Ambient molecular imaging by desorption electrospray ionization mass spectrometry.
2008,
Pubmed
Wiseman,
Tissue imaging at atmospheric pressure using desorption electrospray ionization (DESI) mass spectrometry.
2006,
Pubmed
Wiseman,
Desorption electrospray ionization mass spectrometry: Imaging drugs and metabolites in tissues.
2008,
Pubmed
Wu,
Rapid, direct analysis of cholesterol by charge labeling in reactive desorption electrospray ionization.
2009,
Pubmed
Yanes,
Nanostructure initiator mass spectrometry: tissue imaging and direct biofluid analysis.
2009,
Pubmed
Zhang,
Colloidal graphite-assisted laser desorption/ionization MS and MS(n) of small molecules. 2. Direct profiling and MS imaging of small metabolites from fruits.
2007,
Pubmed
